Lactate wastewater dark fermentation: The effect of temperature and initial pH on biohydrogen production and microbial community

Ziara, Rami M.M.; Miller, Daniel N.; Subbiah, Jeyamkondan; Dvorak, Bruce I.

doi:10.1016/j.ijhydene.2018.11.045

Cited by 83 publications

(17 citation statements)

References 58 publications

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“…Reduction in pH (>4·8) is related to the increase in undissociated acids inhibiting the H 2 producers. The pH is related with methanogen growth limitation and regulation of shift to solventogenesis (Valdez‐Vazquez and Poggi‐Varaldo, ; Ziara et al ), for this reason, the control of pH is important to maintain stable H 2 production.…”

Section: Resultsmentioning

confidence: 99%

The hydraulic retention time influences the abundance of Enterobacter, Clostridium and Lactobacillus during the hydrogen production from food waste

Santiago

Trably

Latrille

et al. 2019

Letters in Applied Microbiology

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The influence of hydraulic retention time (HRT) on the microbial communities was evaluated in an anaerobic sequencing batch reactor (AnSBR) using organic waste from a restaurant as the substrate. The relationship among Lactobacillus, Clostridium and Bacillus as key micro‐organisms on hydrogen production from organic solid waste was studied. The effect of the HRT (8–48 h) on the hydrogen production and the microbial community was evaluated. Quantitative PCR was applied to determine the abundance of bacteria (in particular, Enterobacter, Clostridium and Lactobacillus genera). An AnSBR fermentative reactor was operated for 111 cycles, with carbohydrate and organic matter removal efficiencies of 80 ± 15·42% and 22·1 ± 4·49% respectively. The highest percentage of hydrogen in the biogas (23·2 ± 11·1 %), and the specific production rate (0·42 ± 0·16 mmol H2 gVSadded−1 d−1) were obtained at an HRT of 48 h. The decrease in the HRT generated an increase in the hydrogen production rate but decreasing the content of the hydrogen in the gas. HRT significantly influence the abundance of Enterobacter, Clostridium and Lactobacillus during the hydrogen production from food waste leading the hydrogen production as well as the metabolic pathways. The microbial analysis revealed a direct relationship between the HRT and the presence of fermentative bacteria (Enterobacter, Clostridium and Lactobacillus genera). Clostridium sp. predominated at an HRT of 48 h, while Enterobacter and Lactobacillus predominated at HRTs between 8 and 24 h. Significance and Impact of the Study Significance and Impact of the Study: It was demonstrated that hydrogen production using food waste was influenced by the hydraulic retention time (HRT), and closely related to changes in microbial communities together with differences in metabolic patterns (e.g. volatile fatty acids, lactate, etc.). The decrease in the HRT led to the dominance of lactic acid bacteria within the microbial community whereas the increase in HRT favoured the emergence of Clostridium bacteria and the increase in acetic and butyric acids. Statistical data analysis revealed a direct relationship existing between the HRT and the microbial community composition in fermentative bacteria. This study provides new insight into the relationship between the bioprocess operation and the microbial community to understand better and control the biohydrogen production.

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Section: Resultsmentioning

confidence: 99%

The hydraulic retention time influences the abundance of Enterobacter, Clostridium and Lactobacillus during the hydrogen production from food waste

Santiago

Trably

Latrille

et al. 2019

Letters in Applied Microbiology

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“…IC-76 Municipal wastewater Nitrogen removal efficiency = 96.4% Phosphorus removal efficiency = 77.8% Biomass productivity = 37.18 mg/L/d Lipid content = 36.29% [ 90 ] Saccharomyces cerevisiae MAK-1 Olive mill wastewater Remarkable decolourisation (~ 63%) and phenol removal efficiency (~ 34% (w/w)) Co-production of bioethanol and lipids [ 83 ] Scenedesmus sp. (co-culture of microalgae-bacteria consortium) Starch wastewater (anaerobic sludge) Co-cultivation enhanced biohydrogen production and performed wastewater bioremediation COD removal efficiency = 80.5% Total nitrogen (TN) removal efficiency = 88.7% Total phosphorus (TP) removal efficiency = 80.1% Biohydrogen yield = 1508.3 mL/L Total lipid concentration = 0.36 g/L Energy conversion efficiency = 34.2% [ 91 ] Bacterial consortium ‘Bx’ Textile wastewater contains reactive dye Maximum decolourisation rates = 88–97% Chemical oxygen demand (COD) removal efficiency = 95–98% [ 92 ] PHA-storing and filamentous bacteria Municipal wastewater COD removal efficiency = 70% COD sol concentration removal efficiency = 60% (sol: soluble) Nitrogen removal efficiency = 24% Phosphorus removal efficiency = 46% Co-produced PHA [ 93 ] Microbial community Lactate wastewater (obtained from cattle slaughterhouse) COD removal efficiency = 12–30% Lactate removal efficiency = 54–99.8% Biohydrogen yield = 0.08–0.95 mol H 2 /mol lactate uptake (by dark fermentation process) Identified microbial = Clostridium , Sporanaerobacter and Pseudomonas [ 94 ] Sulphate reducing bacteria consortium Real wastewater from Okhla industrial area effluent Sulphate removal efficiency = 90% Chromium removal efficiency = 82.6% Cadmium removal efficiency = 86.6% Zinc removal efficiency = 54.09% Lead removal efficiency = 49.8% Nickel removal effi...…”

Section: Waste Biorefinery Promoting a Circular Bioeconomymentioning

confidence: 99%

Waste biorefinery towards a sustainable circular bioeconomy: a solution to global issues

Leong

Chang

Khoo

et al. 2021

Biotechnol Biofuels

216

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Global issues such as environmental problems and food security are currently of concern to all of us. Circular bioeconomy is a promising approach towards resolving these global issues. The production of bioenergy and biomaterials can sustain the energy–environment nexus as well as substitute the devoid of petroleum as the production feedstock, thereby contributing to a cleaner and low carbon environment. In addition, assimilation of waste into bioprocesses for the production of useful products and metabolites lead towards a sustainable circular bioeconomy. This review aims to highlight the waste biorefinery as a sustainable bio-based circular economy, and, therefore, promoting a greener environment. Several case studies on the bioprocesses utilising waste for biopolymers and bio-lipids production as well as bioprocesses incorporated with wastewater treatment are well discussed. The strategy of waste biorefinery integrated with circular bioeconomy in the perspectives of unravelling the global issues can help to tackle carbon management and greenhouse gas emissions. A waste biorefinery–circular bioeconomy strategy represents a low carbon economy by reducing greenhouse gases footprint, and holds great prospects for a sustainable and greener world.

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“…Taking the intermediate pyruvate for example, it is metabolized into the liquid metabolites such as acetic acid, propionic acid, butyric acid, valeric acid, and ethanol. 22 The current works of biohydrogen production enhancement using CS as feedstock broadly focus on the following but not the least: (a) improvement of microbial strains, 23,24 (b) construction of the mixed microflora, 25,26 (c) improvement of efficiency of the pretreatment process, 27 and (d) optimization of the pilot scale by coupling the process of DF with the photo fermentation (with the maximum H 2 productivity reaching 60 m 3 / day). 28 However, the works of investigating the effects of acid hydrolysate together with the chemical additions in the aspect of electron-equivalent balances on the corresponding biohydrogen production are much less investigated.…”

Section: Introductionmentioning

confidence: 99%

“…During DF, various hydrogen formations related metabolic pathways undertook either sequentially or simultaneously. Taking the intermediate pyruvate for example, it is metabolized into the liquid metabolites such as acetic acid, propionic acid, butyric acid, valeric acid, and ethanol . The current works of biohydrogen production enhancement using CS as feedstock broadly focus on the following but not the least: (a) improvement of microbial strains, (b) construction of the mixed microflora, (c) improvement of efficiency of the pretreatment process, and (d) optimization of the pilot scale by coupling the process of DF with the photo fermentation (with the maximum H 2 productivity reaching 60 m 3 /day) .…”

Section: Introductionmentioning

confidence: 99%

Optimization of biohydrogen production using acid pretreated corn stover hydrolysate followed by nickel nanoparticle addition

et al. 2019

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Summary The dilute acid hydrolysis using corn stover (CS) to produce reducible sugars was optimized by the response surface methodology. The electron‐equivalent balances of the main metabolites during the dark fermentation (DF) using acid hydrolysate were investigated to identify the evolutions of the electron sinks over the course of DF. The additions of nickel ion and Ni0 nanoparticles (NPs) were found to effectively enhance the hydrogen production at experimental conditions. The optimal condition (HCl 2.5 wt%, hydrolyzing duration 105 minutes, pH=5, S/B=3.5, Ni0 NPs=10 mg/L‐1) was achieved with YH2/S reaching 1.18 (mol.mol‐1‐glucose). The YH2/S increased from 0.7 (mol.mol‐1‐glucose) to 1.18 (mol.mol‐1‐glucose) reaching 40% hydrogen yield increase when Ni0 NPs was added to the fermentation broth. Among the investigated significant soluble metabolites, the butyric acid was found to serve as the largest e‐sink in the electron‐equivalent balance. The additions of Ni0 NPs at low level (below 10 mg/L) were found to appreciably increase the hydrogen production. The increased pH and substrate to biomass ratio were found to skew the metabolic balance from hydrogen production to the biosynthesis (an increase of biomass). The proposed anaerobic digestion model with consideration of the inhibitory factors model presents a good agreement with the experimental data. The chemical addition such as nickel ions, Ni0 NPs was found to be a practical approach in enhancing biohydrogen production using CS acid hydrolysate as cultivation broth.

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Lactate wastewater dark fermentation: The effect of temperature and initial pH on biohydrogen production and microbial community

Cited by 83 publications

References 58 publications

The hydraulic retention time influences the abundance of Enterobacter, Clostridium and Lactobacillus during the hydrogen production from food waste

The hydraulic retention time influences the abundance of Enterobacter, Clostridium and Lactobacillus during the hydrogen production from food waste

Waste biorefinery towards a sustainable circular bioeconomy: a solution to global issues

Optimization of biohydrogen production using acid pretreated corn stover hydrolysate followed by nickel nanoparticle addition

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