Lactate-utilizing bacteria ameliorates DSS-induced colitis in mice

Chen, Lirong; Li, Rong; Wang, Ziguang; Zhang, Zhiwei; Wang, Jie; Qiao, Yuebing; Huang, Yongcun; Liu, Wei

doi:10.1016/j.lfs.2021.120179

Cited by 21 publications

(15 citation statements)

References 49 publications

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“…Lactate can also be a growth substrate for sulfate-reducing gut bacteria [ 25 ]; therefore, it has the potential to promote the formation of toxic concentrations of hydrogen sulfide. Indeed, lactate has been shown to accumulate in the colon of patients with severe colitis [ 26 , 27 , 28 ]. Type D, with the lowest α-diversity, may have fewer lactate-utilizing bacteria.…”

Section: Discussionmentioning

confidence: 99%

Typing of the Gut Microbiota Community in Japanese Subjects

Takagi

Inoüe

Oshima³

et al. 2022

Microorganisms

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Gut microbiota are involved in both host health and disease and can be stratified based on bacteriological composition. However, gut microbiota clustering data are limited for Asians. In this study, fecal microbiota of 1803 Japanese subjects, including 283 healthy individuals, were analyzed by 16S rRNA sequencing and clustered using two models. The association of various diseases with each community type was also assessed. Five and fifteen communities were identified using partitioning around medoids (PAM) and the Dirichlet multinominal mixtures model, respectively. Bacteria exhibiting characteristically high abundance among the PAM-identified types were of the family Ruminococcaceae (Type A) and genera Bacteroides, Blautia, and Faecalibacterium (Type B); Bacteroides, Fusobacterium, and Proteus (Type C); and Bifidobacterium (Type D), and Prevotella (Type E). The most noteworthy community found in the Japanese subjects was the Bifidobacterium-rich community. The odds ratio based on type E, which had the largest population of healthy subjects, revealed that other types (especially types A, C, and D) were highly associated with various diseases, including inflammatory bowel disease, functional gastrointestinal disorder, and lifestyle-related diseases. Gut microbiota community typing reproducibly identified organisms that may represent enterotypes peculiar to Japanese individuals and that are partly different from those of indivuals from Western countries.

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Section: Discussionmentioning

confidence: 99%

Typing of the Gut Microbiota Community in Japanese Subjects

Takagi

Inoüe

Oshima³

et al. 2022

Microorganisms

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“…It revealed in this study that SPH infusion led to an increase in the species richness of colonic microbiota in growing pigs, including an increase in the abundance of protein-fermenting bacteria and a few harmful bacteria. For example, SPH infusion increased the abundance of certain genera, including Erysipelotrichaceae , Prevotellaceae_uncultured , Prevotellaceae UCG-003 , and Candidatus Saccharimonas , which were involved in chronic inflammation [ 13 – 15 ], whereas a few species within the genera Escherichia-Shigella , Dialister , Prevotella , and Veillonella promoted by SPH treatment are regarded as opportunistic pathogens and are relevant to colitis [ 16 – 19 ]. On the other hand, Escherichia-Shigella and Veillonella are the main biogenic amine producers in the colon, while Prevotella and Dialister are associated with the production of branched chain fatty acids and ammonia, respectively [ 16 – 19 ].…”

Section: Discussionmentioning

confidence: 99%

“…For example, SPH infusion increased the abundance of certain genera, including Erysipelotrichaceae , Prevotellaceae_uncultured , Prevotellaceae UCG-003 , and Candidatus Saccharimonas , which were involved in chronic inflammation [ 13 – 15 ], whereas a few species within the genera Escherichia-Shigella , Dialister , Prevotella , and Veillonella promoted by SPH treatment are regarded as opportunistic pathogens and are relevant to colitis [ 16 – 19 ]. On the other hand, Escherichia-Shigella and Veillonella are the main biogenic amine producers in the colon, while Prevotella and Dialister are associated with the production of branched chain fatty acids and ammonia, respectively [ 16 – 19 ]. The findings indicate that SPH increased the abundance of bacteria related to protein fermentation and chronic inflammation.…”

Section: Discussionmentioning

confidence: 99%

Effects of the increased protein level in small intestine on the colonic microbiota, inflammation and barrier function in growing pigs

Ding

Zhu

et al. 2022

BMC Microbiol

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Background An increased level of the dietary protein alters the colonic microbial community and metabolic profile of pigs, but it remains unclear whether this leads to colonic inflammation and impairs barrier function in growing pigs. Results Sixteen pigs (35.2 ± 0.3 kg) were infused with sterile saline (control) or soy protein hydrolysate (SPH) (70 g/day) through a duodenal fistula twice daily during a 15-day experimental period. The SPH treatment did not affect their average daily feed intake and daily weight gain (P > 0.05), but reduced colon index and length (P < 0.05). Illumina MiSeq sequencing revealed that species richness was increased following SPH intervention (P < 0.05). Furthermore, SPH reduced the abundance of butyrate- and propionate-producing bacteria—such as Lachnospiraceae NK4A136 group, Lachnospiraceae_uncultured, Coprococcus 3, Lachnospiraceae UCG-002, and Anaerovibrio—and increased the abundance of potentially pathogenic bacteria and protein-fermenting bacteria, such as Escherichia-Shigella, Dialister, Veillonella, Prevotella, Candidatus Saccharimonas, Erysipelotrichaceae UCG-006, Prevotellaceae_uncultured, and Prevotellaceae UCG-003 (P < 0.05). In addition, a lower content of total short-chain fatty acids, propionate, and butyrate and a higher concentration of cadaverine, putrescine, total biogenic amines, ammonia, and isovalerate were observed following SPH infusion (P < 0.05). Further analysis revealed that SPH increased the concentration of tumour necrosis factor-α, interleukin (IL)-1β, IL-6, and IL-8 in the colonic mucosa (P < 0.05). Interestingly, SPH intervention increased the expression of occludin, zonula occludens (ZO)-1, and claudin-1 in colonic mucosa (P < 0.05). Correlation analysis showed that different genera were significantly related to the production of metabolites and the concentrations of pro-inflammatory cytokines. Conclusion An increased soy protein level in the small intestine altered the colonic microbial composition and metabolic profile, which resulted in the secretion of colonic proinflammatory cytokines and the increased expression of tight junction proteins.

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“…Mice lacking lactate dehydrogenase A, the enzyme responsible for converting pyruvate to lactate, results in a significant decrease in inflammation in models of non-infectious colitis ( 86 ). Furthermore, it has been observed that mice colonized with lactate-utilizing bacteria experience decreased severity of DSS-induced colitis and that administration of these lactate-utilizing bacteria can reverse the dysbiosis associated with colitis ( 87 ).…”

Section: Tissue Acidification and Ph Regulationmentioning

confidence: 99%

The hypoxic tissue microenvironment as a driver of mucosal inflammatory resolution

Cartwright

Colgan

2023

Front. Immunol.

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On the backdrop of all acute inflammatory processes lies the activation of the resolution response. Recent years have witnessed an emerging interest in defining molecular factors that influence the resolution of inflammation. A keystone feature of the mucosal inflammatory microenvironment is hypoxia. The gastrointestinal tract, particularly the colon, exists in a state of physiological hypoxia and during active inflammation, this hypoxic state is enhanced as a result of infiltrating leukocyte oxygen consumption and the activation of oxygen consuming enzymes. Most evidence suggests that mucosal hypoxia promotes the active resolution of inflammation through a variety of mechanisms, including extracellular acidification, purine biosynthesis/salvage, the generation of specialized pro-resolving lipid mediators (ie. resolvins) and altered chemokine/cytokine expression. It is now appreciated that infiltrating innate immune cells (neutrophils, eosinophils, macrophages) have an important role in molding the tissue microenvironment to program an active resolution response. Structural or functional dysregulation of this inflammatory microenvironment can result in the loss of tissue homeostasis and ultimately progression toward chronicity. In this review, we will discuss how inflammatory hypoxia drives mucosal inflammatory resolution and its impact on other microenvironmental factors that influence resolution.

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Lactate-utilizing bacteria ameliorates DSS-induced colitis in mice

Cited by 21 publications

References 49 publications

Typing of the Gut Microbiota Community in Japanese Subjects

Typing of the Gut Microbiota Community in Japanese Subjects

Effects of the increased protein level in small intestine on the colonic microbiota, inflammation and barrier function in growing pigs

The hypoxic tissue microenvironment as a driver of mucosal inflammatory resolution

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