Lactate released from human fibroblasts enhances Ni elution from Ni plate

Kasai, Koji; Segawa, Ryosuke; Onodera, Ryo; Asakawa, Shuichi; Hiratsuka, Masahiro; Hirasawa, Noriyasu

doi:10.1016/j.tox.2021.152723

Cited by 2 publications

(1 citation statement)

References 26 publications

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“…For stimulation or inhibition assays, fibroblasts were cultured in a medium containing TGF-β1 (10 ng/mL, 240-B-002, R&D Systems, Minneapolis) for 48 h, 17 LDN-57444 (UCHL1 inhibitor, S7135, Selleck Chemicals, Houston, TX) for 48 h, 18 MK-2206 2HCl (Akt inhibitor, S1078, Selleck Chemicals, Houston, TX) for 36 h, 19 rapamycin (mTORC1 inhibitor, 553211, Merck Millipore, Billerica, MA) for 24 h, 20 or PX-478 2HCl (HIF-1α inhibitor, S7612, Selleck Chemicals, Houston, TX) for 24 h, 21 and IGF-1 (10598-HNAE, Sino Biological Inc, Beijing, China) for 24 h 22,23 as previously reported.…”

Section: Fibroblast Culture and Treatmentmentioning

confidence: 99%

UCHL1 aggravates skin fibrosis through an IGF‐1‐induced Akt/mTOR/HIF‐1α pathway in keloid

et al. 2023

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Keloids are fibroproliferative dermatoses characterized by the excessive production of extracellular matrix (ECM). The eruptions grow beyond the margin of the original wound and invade the surrounding tissues. The treatment is a great challenge for both clinicians and patients regarding the high frequency of recurrence and resistance. Researches have been amounted recently; however, the pathogenesis still remains poorly understood. 1,2 Fibroblasts are the crucial players in keloids and are featured by great heterogenicity with respect to numerous markers and signaling pathways. The heterogenicity attributes to race, age, location, disease duration, and stage, sampling sites such as margin, center, superficial or deep sites, test methods, and so on. 2 Despite the divergency, alpha-smooth muscle actin (α-SMA) and collagen I, the major extracellular matrix (ECM) components, are the fibrotic markers most frequently used in keloid studies. 3,4 Positive α-SMA fibroblasts are also known as

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