Lactate Dehydrogenase Isoenzymes in Rat Uterus: Changes During Pregnancy

Battellino, Luis José; Sabulsky, J.; Blanco, A.

doi:10.1530/jrf.0.0250393

Cited by 10 publications

(3 citation statements)

References 7 publications

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“…At phase IV the significantly low activity of M-isozymes in uterine fluid of parous women may be due to low levels of endogenous estrogen and to an antagonistic action of progesterone on estrogen induced LDH at this stage (Clark and Yochim 1971). This suggests that significantly high activity of M-isozymes in uterine fluid of parous women from early follicular phase to ovulatory phase (phase I to phase III) compared to that of infertiles, enables transformation of pyruvate into lactate (Battellino, Sabulsky and Blanco 1971), which is an important source of energy for the spermatozoa at this stage (Brinster 1967). Thus estrogen induced luminal M-isozymes enable the uterus to derive maximum energy from lactate in parous women which may help in vital reproductive processes.…”

Section: Discussionmentioning

confidence: 98%

Lactate Dehydrogenase Isozymes in Uterine Fluid of Infertile Women

1994

Horm Metab Res

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Electrophoretic studies revealed predominance of LDH isozymes (LDH4 and LDH5: M-isozymes) throughout the menstrual cycle in the uterine luminal fluid of both parous and infertile women. Parous women M-isozymes maintain higher activity during phase I to phase III (early follicular phase to ovulatory phase) than infertile women. During the post-ovulatory phase (phase IV) the M-isozyme shows lowest level in parous women. In contrast, during this phase (phase IV) to late-luteal phase (phase VI) the M-isozymes show high activity in the fluid milieu of infertile women.

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Section: Discussionmentioning

confidence: 98%

Lactate Dehydrogenase Isozymes in Uterine Fluid of Infertile Women

1994

Horm Metab Res

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“…Hesseldeheer [30] observed a 50%-point increase in lactate dehydrogenase concentration in plasma after forcing pigs to exercise for 5 min, compared to a non-forced exercise group. Moreover, a rat study showed an increased e ciency of lactate dehydrogenase with increased level of muscle activities [31]. Challenging neonate piglets for 2 h at 10°C below their normal thermoneutral zone is considered as an extreme environmental condition which increases skeletal muscle shivering and, thereby also increase the lactate dehydrogenase concentration in the blood.…”

Section: Discussionmentioning

confidence: 99%

Supplementation of palmitoleic acid improved piglet growth and reduced body temperature drop upon cold exposure

Feyera,

Lashkari,

Johannsen

et al. 2023

Journal of Animal Science

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Piglet survival is a major challenge in the first few days postpartum and interventions during this period may improve survival and growth. This study investigated the effects of palmitoleic acid (C16:1n-7; PA) supplementation on growth performance, body temperature, fatty acid (FA), and energy metabolism in milk-replacer-fed piglets. Forty-eight piglets were stratified by body weight and randomly assigned to one of four dietary treatments (0%, 1%, 2%, and 3% PA supplementation as a percent of milk replacer) and given the diet through an orogastric tube. They were fed dietary treatments every 2 h for 4 d in the first week postpartum and all were sacrificed at the end of the experiment. The piglets were weighed daily, and half in each dietary treatment group, the same piglets each day, were exposed daily to a lower temperature for 2 h. Plasma samples were collected immediately before sacrifice for analyses of FA and other plasma metabolites. The weight of organs and empty body weight were determined after sacrifice. Liver and semimembranosus muscle tissue samples were collected and analyzed for FA content. Contents of C16:1n-7 and C18:1n-7 in both plasma and liver (P < 0.001), and C16:1n-7 in semimembranosus muscle (P < 0.001) increased linearly as PA supplementation increased. Most plasma FA levels (except C16:1n-7, C16:1n-9, and C22:5n-3) were lower in piglets exposed to lower temperatures than those that were not. Plasma glucose, triglycerides, and lactate dehydrogenase levels increased linearly with PA supplementation (P < 0.001). Piglets’ average daily gain, liver glycogen pool, liver weight, and gallbladder weight increased linearly (P < 0.05, P < 0.01, P < 0.05, and P < 0.001, respectively), but lung weight, liver nitrogen content, and body temperature drop decreased linearly (P < 0.01, P < 0.001, and P < 0.05, respectively) with PA supplementation. Piglets exposed to low temperature had greater liver nitrogen (P < 0.05) and lactate dehydrogenase (P < 0.001) contents but had lower liver weight (P < 0.01) and plasma lactate concentration (P < 0.05) than those that were not. In conclusion, this study demonstrated the importance of PA on the growth performance of the piglets by increasing their average daily gain and decreasing a drop in body temperature upon cold exposure, most likely due to a modified energy metabolism.

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“…Rise in M-isozymes of LDH at DAM-i and DAM-2 also enables the uterus to transform pyruvate into lactate which is the only source of energy through glycolyte pathway during anaerobic condition (1) for the survival of male and female gametes in uterine lumen. Accumulation of lactate results in low oxygen tension when Misozymes of LDH dominate in the uterine fluid and thus initiates functioning of glycolytic pathway (2,4).…”

Section: Discussionmentioning

confidence: 99%

Changes in LDH Isozyme Pattern in Uterine Fluid of Mice During Early Pregnancy

Np¹,

Singh²,

Vn³

1997

Horm Metab Res

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The total LDH activity in the uterine fluid of mice shows a rising trend from the first day after mating (DAM-1) to the seventh day after mating (DAM-7). This suggests that LDH activity increases as gestation progresses. During early pregnancy, M-isozymes of LDH show a predominance from DAM-1 to DAM-3 in the uterine luminal fluid of mice, while H-isozyme shows a rising level from DAM-5 (implantation day) and attains a maximum level at DAM-7 (the post-implantation period). Such shift of M-isozymes into H-isozymes of LDH (lactate dehydrogenase) during pre-implantation (DAM-3) to post-implantation (DAM-7) period changes the uterine luminal environment from anaerobic to aerobic condition which is more conducive for the normal development, implantation and survival of the growing blastocyst.

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Lactate Dehydrogenase Isoenzymes in Rat Uterus: Changes During Pregnancy

Cited by 10 publications

References 7 publications

Lactate Dehydrogenase Isozymes in Uterine Fluid of Infertile Women

Lactate Dehydrogenase Isozymes in Uterine Fluid of Infertile Women

Supplementation of palmitoleic acid improved piglet growth and reduced body temperature drop upon cold exposure

Changes in LDH Isozyme Pattern in Uterine Fluid of Mice During Early Pregnancy

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