Lactate Activates Germline and Cleavage Embryo Genes in Mouse Embryonic Stem Cells

Tian, Qing; Zhou, Li-Quan

doi:10.3390/cells11030548

Cited by 27 publications

(24 citation statements)

References 53 publications

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“…Followed by the metabolic remodeling, Glis1 quickly activates glycolysis, thus enhancing histone lactylation (H3K18la), opening pluripotency genes, promoting senescent cell reprogramming and improving the genome stability of the induced pluripotent stem cells. Similarly, another study observed the upregulation of germline and cleavage embryo genes in mouse embryonic stem cells after the accumulation of histone Kla (Tian and Zhou, 2022). Further analysis revealed that the remarkably increased deposition of H3K18la at the transcription start site might be responsible for the sequential activation of germline and zygotic genome activation genes.…”

Section: Lactylation and Cell Fate Determinationmentioning

confidence: 80%

“…Of note, during somatic reprogramming, H3K18la tended to be highly enriched at the promoters of pluripotency genes, such as Octamer-binding transcription factor 4 (Oct4), spalt like transcription factor 4 (Sall4) and MYCN proto-oncogene (Mycn), which subsequently upregulated their expressions (Li et al, 2020). In contrast, the expression of pluripotent genes, including Oct4, Nanog homeobox (Nanog), and SRY-box transcription factor 2 (Sox2), were not affected upon lactate supplementation in mouse embryonic stem cells (Tian and Zhou, 2022). That is, lactate strengthened H3K18la without affecting pluripotency of mouse embryonic stem cells.…”

Section: Lactylation and Cell Fate Determinationmentioning

confidence: 98%

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Lactylation, an emerging hallmark of metabolic reprogramming: Current progress and open challenges

Zhou

2022

Front. Cell Dev. Biol.

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Lactate, the end product of glycolysis, efficiently functions as the carbon source, signaling molecules and immune regulators. Lactylation, being regulated by lactate, has recently been confirmed as a novel contributor to epigenetic landscape, not only opening a new era for in-depth exploration of lactate metabolism but also offering key breakpoints for further functional and mechanistic research. Several studies have identified the pivotal role of protein lactylation in cell fate determination, embryonic development, inflammation, cancer, and neuropsychiatric disorders. This review summarized recent advances with respect to the discovery, the derivation, the cross-species landscape, and the diverse functions of lactylation. Further, we thoroughly discussed the discrepancies and limitations in available studies, providing optimal perspectives for future research.

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Section: Lactylation and Cell Fate Determinationmentioning

confidence: 80%

Section: Lactylation and Cell Fate Determinationmentioning

confidence: 98%

Lactylation, an emerging hallmark of metabolic reprogramming: Current progress and open challenges

Zhou

2022

Front. Cell Dev. Biol.

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“…Additionally, hypoxia reduces the activity of LDHA, which reduces lactate production. Moreover, another study showed that the accumulation of H3K18la on germline and cleavage embryo genes promotes transcriptional elongation ( Tian and Zhou, 2022 ). The authors found that adding lactate activated the germline genes and cleavage embryo genes in mouse embryonic stem cells (ESCs).…”

Section: Lactylation and Diseasesmentioning

confidence: 99%

The role and mechanism of histone lactylation in health and diseases

Xie

Liu

et al. 2022

Front. Genet.

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Whether under anaerobic or aerobic conditions, glycolysis results in production of lactate. Increasing evidence suggests that lactate serves as a multifunctional signaling molecule that develops non-metabolic activities in addition to serving as a key metabolite to link glycolysis and oxidative phosphorylation. Histone posttranslational modification patterns (HPTMs) are essential epigenetic processes controlling a variety of biological activities. Proteomics based on mass spectrometry (MS) has been used to progressively reveal new HPTMs. Recent discoveries of histone lactylation modification mediated by lactate and subsequent research demonstrating its involvement in cancer, inflammation, lung fibrosis, and other conditions suggest that it plays a significant role in immune regulation and homeostasis maintenance. This review provides a brief overview of the complicated control of histone lactylation modification in both pathological and physiological conditions.

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Section: The Strategies Used For Inducing 2clcs May Improve Scnt Effi...mentioning

confidence: 99%

“…It was recently revealed that lactate and lactate-induced histone lactylation are also involved in cell fate conversion in mouse ESCs [ 86 ]. Exogenous lactate stimulates the lactylation of histone lysine residues [ 87 ], and lactate treatment for 24 h significantly improved lactylation of H3K18 (H3K18la) in ESCs [ 86 ]. Findings from chromatin immunoprecipitation-sequencing and RNA-sequencing analysis of mouse ESCs found a positive correlation between H3K18la intensity and transcript abundance.…”

Section: The Strategies Used For Inducing 2clcs May Improve Scnt Effi...mentioning

confidence: 99%

2-Cell-like Cells: An Avenue for Improving SCNT Efficiency

Liu

2022

Biomolecules

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After fertilization, the zygote genome undergoes dramatic structural reorganization to ensure the establishment of totipotency, and then the totipotent potential of the zygote or 2-cell-stage embryo progressively declines. However, cellular potency is not always a one-way street. Specifically, a small number of embryonic stem cells (ESCs) occasionally overcome epigenetic barriers and transiently convert to a totipotent status. Despite the significant potential of the somatic cell nuclear transfer (SCNT) technique, the establishment of totipotency is often deficient in cloned embryos. Because of this phenomenon, the question arises as to whether strategies attempting to induce 2-cell-like cells (2CLCs) can provide practical applications, such as reprogramming of somatic cell nuclei. Inspired by strategies that convert ESCs into 2CLCs, we hypothesized that there will be a similar pathway by which cloned embryos can establish totipotent status after SCNT. In this review, we provide a snapshot of the practical strategies utilized to induce 2CLCs during investigations of the development of cloned embryos. The 2CLCs have similar transcriptome and chromatin features to that of 2-cell-stage embryos, and we propose that 2CLCs, already a valuable in vitro model for dissecting totipotency, will provide new opportunities to improve SCNT efficiency.

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Lactate Activates Germline and Cleavage Embryo Genes in Mouse Embryonic Stem Cells

Cited by 27 publications

References 53 publications

Lactylation, an emerging hallmark of metabolic reprogramming: Current progress and open challenges

Lactylation, an emerging hallmark of metabolic reprogramming: Current progress and open challenges

The role and mechanism of histone lactylation in health and diseases

2-Cell-like Cells: An Avenue for Improving SCNT Efficiency

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