Lactase (β-galactosidase) immobilization by complex formation: Impact of biopolymers on enzyme activity

Souza, Clitor J.F.; Garcia‐Rojas, Edwin Elard; Favaro-Trindade, Carmen Sílvia

doi:10.1016/j.foodhyd.2018.04.044

Cited by 39 publications

(15 citation statements)

References 48 publications

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“…This is consistent with the known excellent catalytic activity and selectivity of this zeolite for the epoxidation of light olefins in water (36,81,82). The level of activity is also in line with previously reported data (35,75).…”

Section: Study Of the Inorganic Catalyst (Ts-1 And Aer_ts-1)supporting

confidence: 92%

“…For example, Maruyama et al and Izaki et al have both shown, respectively with protein-polyelectrolyte complexes and antibody-polyelectrolyte complexes, that the formation of these complexes leads to a better protein stabilization against thermally-induced denaturation and mechanical stress (73,74). Souza et al also highlighted a decrease in the denaturation rate of complexed β-galactoxidase compared to the free enzyme when exposed to low pH (75). These results prompted us to investigate the possible stabilization of GOx through the formation of EPCs, in the perspective of the preparation of a GOx/TS-1 hybrid catalyst by one-step spray drying.…”

Section: Introductionmentioning

confidence: 96%

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Hybrid Chemo-Biocatalysts Prepared in One Step from Zeolite Nanocrystals and Enzyme-Polyelectrolyte Complexes

Verren¹,

Smeets²,

Straeten³

et al. 2020

Preprint

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The combination of heterogeneous catalysts and enzymes, in so-called hybrid catalysts, is an attractive strategy to effectively run chemoenzymatic reactions. Yet, the preparation of such bifunctional materials remains challenging because both the inorganic and the biological moieties must be integrated in the same solid, while preserving their intrinsic activity. Combining an enzyme and a zeolite, for example, is complicated because the pores of the zeolite are too small to accommodate the enzyme and a covalent anchorage on the surface is often ineffective. Herein, we developed a new pathway to prepare a hybrid catalyst built from glucose oxidase and TS-1 zeolite. Such hybrid material can catalyze the in situ formation of H2O2, which is subsequently used by the zeolite to trigger the epoxidation of allylic alcohol. Starting from an enzymatic solution and a suspension of zeolite nanocrystals, the hybrid catalyst is obtained in one step, using a continuous spray drying method. While enzymes are expectedly unable to resist the conditions used in spray drying (temperature, shear stress, etc.), we leverage on the preparation of “enzyme-polyelectrolyte complexes” (EPCs) to increase the enzyme stability. Importantly, the use of EPCs also appears to prevent enzyme leaching and to stabilize the enzyme against pH changes. We show that the one-pot preparation by spray drying gives access to hybrid catalysts with unprecedented performance in the targeted chemoenzymatic reaction. Interestingly, the hybrid catalyst performs much better than the two catalysts operating as separate entities. We anticipate that this strategy could be used as an adaptable method to prepare other types of multifunctional materials.<br>

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Section: Study Of the Inorganic Catalyst (Ts-1 And Aer_ts-1)supporting

confidence: 92%

Section: Introductionmentioning

confidence: 96%

Hybrid Chemo-Biocatalysts Prepared in One Step from Zeolite Nanocrystals and Enzyme-Polyelectrolyte Complexes

Verren¹,

Smeets²,

Straeten³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…74,75 Souza et al also highlighted a decrease in the denaturation rate of complexed b-galactoxidase compared to the free enzyme when exposed to low pH. 76 These results prompted us to investigate the possible stabilization of GOx through the formation of EPCs, in the perspective of the preparation of a GOx/TS-1 hybrid catalyst by one-step spray drying.…”

Section: Introductionmentioning

confidence: 99%

Hybrid chemoenzymatic heterogeneous catalyst prepared in one step from zeolite nanocrystals and enzyme–polyelectrolyte complexes

et al. 2021

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“…Strains with β-galactosidase activity were screened by adding 20 mg/ml 5-bromo-4-chloro-3-indolyl-β- D -galactopyranoside (Sangon Biological Technology Co., Ltd., Shanghai, China) to YPD agar plates, where colonies testing positive for β-galactosidase activity turned blue. Measurement of β-galactosidase activity was based on the method of Souza et al (2018) . Furthermore, 50 μl enzyme solution was added to 50 μl 20 mM o -nitrophenyl-β- D -galactopyranoside substrate solution (Sangon Biological Technology), and the mixture was then incubated for 10 min at 37°C in a water bath (HH-1 Digital Thermostatic Water Bath; Junteng Electronic Instrument Co., Ltd., Shandong, China).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Effect of Auxiliary Starter Yeasts With Enzyme Activities on Kazak Cheese Quality and Flavor

Xiao

Chen

et al. 2020

Front. Microbiol.

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To investigate the effect of yeasts on Kazak cheese quality and flavor, three isolated yeasts (Kluyveromyces marxianus A2, Pichia kudriavzevii A11, and Pichia fermentans A19) were used to ferment cheeses and designated as StC, LhC, and WcC, respectively. The cheese fermented with a commercial lactic acid starter without adding yeast was used as control named LrC. The results showed that the texture of cheese added with yeasts were more brittle. K. marxianus A2 contributed to the formation of free amino acids and organic acids, especially glutamate and lactic acid. Moreover, K. marxianus A2 provides cheese with onion, oily, and floral aromas. Furthermore, P. kudriavzevii A11 promotes a strong brandy, herbaceous, and onion flavor. Although no significant aroma change was observed in PfC, it promoted the production of acetic acid, isoamyl acetate, and phenethyl acetate. These results indicate that yeasts are important auxiliary starters for cheese production.

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Lactase (β-galactosidase) immobilization by complex formation: Impact of biopolymers on enzyme activity

Cited by 39 publications

References 48 publications

Hybrid Chemo-Biocatalysts Prepared in One Step from Zeolite Nanocrystals and Enzyme-Polyelectrolyte Complexes

Hybrid Chemo-Biocatalysts Prepared in One Step from Zeolite Nanocrystals and Enzyme-Polyelectrolyte Complexes

Hybrid chemoenzymatic heterogeneous catalyst prepared in one step from zeolite nanocrystals and enzyme–polyelectrolyte complexes

Evaluation of the Effect of Auxiliary Starter Yeasts With Enzyme Activities on Kazak Cheese Quality and Flavor

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