Lactase Deficiency and Lactose Malabsorption

Cooper, Barrett R.

doi:10.1159/000171139

Cited by 7 publications

(2 citation statements)

References 28 publications

(52 reference statements)

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Section: Researchmentioning

confidence: 99%

“…The undigested lactose in the small intestine accelerates the intestinal transit; reduces water absorption; and is metabolized by colonic bacteria into volatile short-chain fatty acids, hydrogen, and carbon dioxide. This provokes the typical clinical symptoms of hypolactasia: abdominal pain, nausea, and diarrhea, which may subsequently provoke severe dehydration 8,9 .To circumvent the hypolactasia symptoms occurring after milk ingestion, several expensive and time-consuming methods have been developed to get low-lactose milk in vitro (i.e., ultracentrifugation or treatment with soluble or immobilized fungal ␤-galactosidases) 5,9 . We have investigated a way to produce low-lactose milk in vivo instead of in vitro, by expressing a lactose-hydrolyzing enzyme in the mammary gland.…”

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Production of low-lactose milk by ectopic expression of intestinal lactase in the mouse mammary gland

et al. 1999

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We have investigated, in mice, an in vivo method for producing low-lactose milk, based on the creation of transgenic animals carrying a hybrid gene in which the intestinal lactase-phlorizin hydrolase cDNA was placed under the control of the mammary-specific alpha-lactalbumin promoter. Transgenic females expressed lactase protein and activity during lactation at the apical side of mammary alveolar cells. Active lactase was also secreted into milk, anchored in the outer membrane of fat globules. Lactase synthesis in the mammary gland caused a significant decrease in milk lactose (50-85%) without obvious changes in fat and protein concentrations. Sucklings nourished with low-lactose milk developed normally. Hence, these data validate the use of transgenic animals expressing lactase in the mammary gland to produce low-lactose milk in vivo, and they demonstrate that the secretion of an intestinal digestive enzyme into milk can selectively modify its composition.

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Section: Researchmentioning

confidence: 99%

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confidence: 99%

Production of low-lactose milk by ectopic expression of intestinal lactase in the mouse mammary gland

et al. 1999

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Intolérance au lactose et déficit en lactase intestinale: aspects physiopathologiques, nutritionnels et biochimiques

Raul

1988

Nutrition Clinique et Métabolisme

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Human Adult-Onset Lactase Decline: An Update

Lee

Krasinski

2009

Nutrition Reviews

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Human adult-onset lactase decline is a biologic feature characteristic of the maturing intestine in the majority of the world's population. The digestion and absorption of lactose, the major carbohydrate in milk and also the main substrate for lactase, is often variable, a consequence of lactase levels, gastric emptying rate, and colonic salvage. Although commercially available "lactase " products alleviate symptoms in many lactoseintolerant people, a greater understanding of this variability in lactose tolerance could lead to interventions that reduce the rate of gastric emptying andlor increase the proliferation of lactose-metabolizing bacteria in the colon, leading to more efficient lactose utilization. Adult-onset lactase decline appears to be a risk factor for developing osteoporosis, owing to avoidance of dairy products or interference of undigested lactose with calcium absorption. Elderly with both adult-onset lactase decline and atrophic gastritis or those undergoing anti-ulcer treatment may have an increased risk of low calcium absorption owing to the lack of gastric acid that facilitates calcium uptake. Thus, lactose-intolerant elders should monitor their calcium nutrition status carefully.

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Lactase Deficiency and Lactose Malabsorption

Cited by 7 publications

References 28 publications

Production of low-lactose milk by ectopic expression of intestinal lactase in the mouse mammary gland

Production of low-lactose milk by ectopic expression of intestinal lactase in the mouse mammary gland

Intolérance au lactose et déficit en lactase intestinale: aspects physiopathologiques, nutritionnels et biochimiques

Human Adult-Onset Lactase Decline: An Update

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