-Lactam and aminoglycoside resistance rates and mechanisms among Pseudomonas aeruginosa in French general practice (community and private healthcare centres)

Dubois, Véronique; Arpin, Corinne; Dupart, Virginie; Scavelli, Aline; Coulange, Laure; André, Catherine; Fischer, Isabelle; Grobost, F.; Brochet, Jean-Philippe; Lagrange, Isabelle; Dutilh, B; Jullin, Jacqueline; Noury, Patrick; Larribet, G.; Quentin, Claudine

doi:10.1093/jac/dkn174

Cited by 55 publications

(37 citation statements)

References 25 publications

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“…Highly plausible is the presence of other mechanisms resistance to aminoglycoside antibiotics, both in the population of strains aacA4-positive and aacA4-negative [7].…”

Section: Resultsmentioning

confidence: 99%

Occurrence of the aacA4 gene among multidrug resistant strains of Pseudomonas aeruginosa isolated from bronchial secretions obtained from the Intensive Therapy Unit at University Hospital in Bialystok, Poland

Sacha

Jaworowska

Ojdana

et al. 2012

Folia Histochem Cytobiol.

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“…Highly plausible is the presence of other mechanisms resistance to aminoglycoside antibiotics, both in the population of strains aacA4-positive and aacA4-negative [7].…”

Section: Resultsmentioning

confidence: 99%

Occurrence of the aacA4 gene among multidrug resistant strains of Pseudomonas aeruginosa isolated from bronchial secretions obtained from the Intensive Therapy Unit at University Hospital in Bialystok, Poland

Sacha

Jaworowska

Ojdana

et al. 2012

Folia Histochem Cytobiol.

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“…According to Forest Laboratories, studies of ceftaroline in combination with NXL104 are currently in progress (1). For P. aeruginosa isolates, against which ceftaroline has limited activity, high levels of resistance to ceftaroline and other ␤-lactam drugs may be due to (i) a decreased affinity for the PBPs, (ii) a failure to achieve inhibitory concentrations due to impermeability or efflux (via the MexXY efflux pump, for example), and/or (iii) enzymatic hydrolysis resistant to ␤-lactamase inhibitors (4,8,10). Studies of the mechanisms of resistance responsible for the reduced activity of ceftaroline against P. aeruginosa isolates are still warranted.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Activity of Ceftaroline Alone and in Combination against Clinical Isolates of Resistant Gram-Negative Pathogens, Including β-Lactamase-Producing Enterobacteriaceae and Pseudomonas aeruginosa

Vidaillac

Leonard

Sader

et al. 2009

Antimicrob Agents Chemother

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Ceftaroline is a novel broad-spectrum cephalosporin that exhibits bactericidal activity against many grampositive and -negative pathogens. However, the activity of ceftaroline cannot be solely relied upon for eradication of multidrug-resistant gram-negative isolates, such as Pseudomonas aeruginosa and extended-spectrum ␤-lactamase (ESBL)-producing Enterobacteriaceae, which represent a current clinical concern. As drug combinations might be beneficial by potential synergy, we evaluated the in vitro activity of ceftaroline combined with meropenem, aztreonam, cefepime, tazobactam, amikacin, levofloxacin, and tigecycline. Susceptibility testing was performed for 20 clinical P. aeruginosa isolates, 10 ESBL-producing Escherichia coli isolates, 10 ESBL-producing Klebsiella pneumoniae isolates, and 10 AmpC-derepressed Enterobacter cloacae isolates. Timekill experiments were performed for 10 isolates using antimicrobials at one-fourth the MIC. Ceftaroline exhibited a MIC range of 0.125 to 1,024 g/ml and was reduced 2-to 512-fold by combination with tazobactam (4 g/ml) for ESBL-producing strains. In time-kill experiments, ceftaroline plus amikacin was synergistic against 90% of the isolates (and indifferent for one P. aeruginosa isolate). Ceftaroline plus tazobactam was indifferent for E. cloacae and P. aeruginosa strains but synergistic against 100% of E. coli and K. pneumoniae isolates. Combinations of ceftaroline plus meropenem or aztreonam were also synergistic for all E. coli and E. cloacae isolates, respectively, but indifferent against 90% of the other isolates. Finally, combinations of ceftaroline plus either tigecycline, levofloxacin, or cefepime were indifferent for 100% of the isolates. No antagonism was observed with any combination. Ceftaroline plus amikacin appeared as the most likely synergistic combination. This represents a promising therapeutic option, and further studies are warranted to elucidate the clinical value of ceftaroline combinations against resistant gram-negative pathogens.

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“…The presence of aminoglycoside-modifying enzymes (AMEs) was also assessed by PCR according to previously described protocols (15)(16)(17). In all cases, the acetyltransferase gene aac(6=)-Ib was amplified.…”

mentioning

confidence: 99%

Isolation of VIM-2-Producing Pseudomonas monteilii Clinical Strains Disseminated in a Tertiary Hospital in Northern Spain

Ocampo-Sosa

Guzmán-Gómez

Fernández-Martínez

et al. 2015

Antimicrob Agents Chemother

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We describe here the occurrence of bla VIM-2 in 10 carbapenem-resistant Pseudomonas monteilii strains isolated from different clinical samples from patients at the University Hospital Marqués de Valdecilla in northern Spain. All the bla VIM-2 -harboring P. monteilii isolates possessed a class 1 integron, with the cassette array [intI1_bla VIM-2 _aac(6=)-Ib_qacE⌬1_sul1]. Our results show the emergence of VIM-2-producing multidrug-resistant species other than Pseudomonas aeruginosa or Pseudomonas putida in a Spanish hospital. P. monteilii, although sporadically isolated, should also be considered an important metallo-␤-lactamase (MBL) reservoir.

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-Lactam and aminoglycoside resistance rates and mechanisms among Pseudomonas aeruginosa in French general practice (community and private healthcare centres)

Abstract: Antibiotic resistance rates in P. aeruginosa were globally similar in general practice as in French hospitals. This first analysis of resistance mechanisms showed an unexpectedly high frequency of ESBLs and an unusual distribution of aminoglycoside modifying enzymes.

Cited by 55 publications

References 25 publications

Occurrence of the <i>aacA4</i> gene among multidrug resistant strains of <i>Pseudomonas aeruginosa</i> isolated from bronchial secretions obtained from the Intensive Therapy Unit at University Hospital in Bialystok, Poland

Occurrence of the <i>aacA4</i> gene among multidrug resistant strains of <i>Pseudomonas aeruginosa</i> isolated from bronchial secretions obtained from the Intensive Therapy Unit at University Hospital in Bialystok, Poland

In Vitro Activity of Ceftaroline Alone and in Combination against Clinical Isolates of Resistant Gram-Negative Pathogens, Including β-Lactamase-Producing Enterobacteriaceae and Pseudomonas aeruginosa

Isolation of VIM-2-Producing Pseudomonas monteilii Clinical Strains Disseminated in a Tertiary Hospital in Northern Spain

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