Lack of UDS activity in the livers of rats exposed to allylisothiocyanate

Bechtel, David; Henderson, L.M.; Proudlock, Raymond

doi:10.1002/(sici)1520-6866(1998)18:5<209::aid-tcm1>3.0.co;2-6

Cited by 15 publications

(6 citation statements)

References 26 publications

(53 reference statements)

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“…Nor did AITC cause significant chromosome aberrations or sister chromatic exchanges in a SV40‐transformed Indian muntjac cell line and a Chinese hamster ovary cell line even at highly cytotoxic doses 72, 73. Similarly, unscheduled DNA synthesis was not detected in the livers of Sprague–Dawley rats receiving a single oral dose of AITC up to 125 mg/kg body weight (1.25 mmol/kg) 74.…”

Section: The Dichotomy Of Cytoprotective Activity and Toxicity Of Aitcmentioning

confidence: 95%

Allyl isothiocyanate as a cancer chemopreventive phytochemical

Zhang

2010

Molecular Nutrition Food Res

206

162

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Allyl isothiocyanate (AITC), which occurs in many common cruciferous vegetables, is widely and often frequently consumed by humans. Besides antimicrobial activity against a wide spectrum of pathogens, it showed anticancer activity in both cultured cancer cells and animal models, although the underlining mechanisms remain largely undefined. Bioavailability of AITC is extremely high, as nearly 90% of orally administered AITC is absorbed. AITC absorbed in vivo is metabolized mainly through the mercapturic acid pathway and excreted in urine. Available data suggest that urinary concentrations of AITC equivalent are at least 10 times higher than in the plasma, and tissue levels of AITC equivalent in the urinary bladder were 14-79 times higher than in other organs after oral AITC administration to rats. These findings suggest that AITC may be most effective in the bladder as a cancer chemopreventive compound. AITC at high dose levels also exhibit a low degree of cytotoxicity and genotoxicity in animal studies, but such adverse effects are unlikely in humans exposed to dietary levels of AITC. Overall, AITC exhibits many desirable attributes of a cancer chemopreventive agent, and further studies are warranted in order to elucidate its mechanism of action and to assess its protective activity in humans.

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Section: The Dichotomy Of Cytoprotective Activity and Toxicity Of Aitcmentioning

confidence: 95%

Allyl isothiocyanate as a cancer chemopreventive phytochemical

Zhang

2010

Molecular Nutrition Food Res

206

162

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“…These include diallyl disulfide, which is the major component of garlic oil, and allyl isothiocyanate, which can be derived from its glucosinolate precursor in mustard and broccoli. Although there have been some reports of the mutagenicity of allyl isothiocyanate, 6,7 studies in rats led Bechtel et al 8 to conclude that the weight of evidence is insufficient to regard allyl isothiocyanate as a genotoxin capable of human carcinogenicity. Growth inhibitory effects of allyl isothiocyanate on cancer cells have been reported.…”

mentioning

confidence: 99%

Induction of histone acetylation in mouse erythroleukemia cells by some organosulfur compounds including allyl isothiocyanate

et al. 2001

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In previous studies we observed that some allyl sulfides can cause increased acetylation of histones and differentiation in DS19 mouse erythroleukemia cells. In the present work we observed increased acetylation of histones with allyl isothiocyanate and butanethiol but not with butyl sulfide or butyl disulfide. Increased acetylation of histones was established by change in electrophoretic mobility, incorporation of [ 3 H]acetate or immunoblotting. Histone deacetylase in nuclei of DS19 cells was inhibited 74% by 0.5 mM allyl mercaptan and 43% by 0.5 mM butanethiol but was not significantly affected by 0.5 mM allyl isothiocyanate. There was some degree of reversibility in the effect of allyl isothiocyanate when the cells were incubated for 15 hr in fresh medium. The data suggested that allyl isothiocyanate may stimulate histone acetylation rather than inhibit histone deacetylation. Addition of allyl isothiocyanate, however, had very little or no additional effect on the induction of histone acetylation caused by trichostatin A. Histone acetyltransferase activity determined in cell homogenates was not increased by preincubation of cells with allyl isothiocyanate or inclusion of allyl isothiocyanate in the assay medium. It was concluded that treatment of mouse erythroleukemia cells with allyl isothiocyanate can cause increased acetylation of histones but the mechanism for this effect requires further elucidation.

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“…In groups of four male Hsd/Ola Sprague-Dawley rats, allyl isothiocyanate, administered once by gavage at a dose of 37.5 or 125 mg/kg bw, did not induce unscheduled DNA synthesis in hepatocytes (examined by autoradiography) 2 and 14 h after treatment. Although a significant increase in gross nuclear and cytoplasmic grain counts was observed at the higher dose at the later sampling time, this was not considered to be unscheduled DNA synthesis as there was no increased net nuclear grain count (Bechtel et al, 1998).…”

Section: In Vivomentioning

confidence: 99%

“…Sex-linked recessive lethal mutations Drosophila melanogaster (male) NR t Weakly positive (Auerbach & Robson, 1947) Sex-linked recessive lethal mutations Drosophila melanogaster (male) 650 ppm u Negative (Valencia et al, 1985) Sex-linked recessive lethal mutations Drosophila melanogaster (male) 54 ppm u Negative (Zimmering et al, 1989) Sex-linked recessive lethal mutations Drosophila melanogaster (male) 700 ppm v Negative (Valencia et al, 1985) Chromosome breaks Drosophila melanogaster NR t Negative (Auerbach & Robson, 1947) (Bechtel et al, 1998) The EFSA Journal (2008) Toxicity data avaible to establish an adequate NOAEL. No safety concern at estimated level of intake as flavouring substance based on the MSDI approach.…”

Section: Micronucleus Inductionmentioning

confidence: 99%

Flavouring Group Evaluation 85: Consideration of miscellaneous nitrogen-containing substances evaluated by JECFA (65th meeting) - Scientific Opinion of the Scientific Panel on Food Additives, Flavourings, Processing Aids and Materials in Contact with Food

Flavourings¹

2008

EFSA Journal

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Lack of UDS activity in the livers of rats exposed to allylisothiocyanate

Cited by 15 publications

References 26 publications

Allyl isothiocyanate as a cancer chemopreventive phytochemical

Allyl isothiocyanate as a cancer chemopreventive phytochemical

Induction of histone acetylation in mouse erythroleukemia cells by some organosulfur compounds including allyl isothiocyanate

Flavouring Group Evaluation 85: Consideration of miscellaneous nitrogen-containing substances evaluated by JECFA (65th meeting) - Scientific Opinion of the Scientific Panel on Food Additives, Flavourings, Processing Aids and Materials in Contact with Food

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