Lack of Staphylococcal Enterotoxin Production among Strains of Staphylococcus aureus from Bovine Mastitis in Denmark

Aarestrup, F. M.; Andersen, Jens Kirk; Jensen, N. E.

doi:10.1186/bf03547695

Cited by 16 publications

(4 citation statements)

References 12 publications

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“…In humans, nosocomial and community-acquired staphylococcal infections have been most commonly reported [2]. Coagulase-positive (CPS) and coagulase-negative staphylococci (CNS) are important mastitis pathogens in animals [3,4].…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and Genotypic Antibiotic Resistance Patterns of Staphylococcus aureus From Raw and Spontaneously Fermented Camel Milk

Njage

2013

EJNFS

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Aims: To pheno-and genotypically characterise Staphylococcus aureus isolated from raw and fermented camel milk from Kenya and Somali for their antibiotic resistance. Methodology: Microdilution assays to determine minimal inhibitory concentrations (MICs) were done using to 20 different antibiotics. Further tests with selected antibiotics were done using disk diffusion test. Genotypic antibiotic resistance was tested using by microarray hybridization with selected isolates and consequent screening of antibiotic resistance genes by PCR. Results: Prevalence of antibiotic resistance among the 47 S. aureus tested were ampicillin 26% (12), gentamicin 26% (12), streptomycin 11% (5), tetracycline 13% (6), trimethoprim 6% (3) and fusidic acid 2% (1). Multi-resistance was detected with three isolates resistant to two antibiotics, six to three antibiotics and six to four or more

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Section: Introductionmentioning

confidence: 99%

Phenotypic and Genotypic Antibiotic Resistance Patterns of Staphylococcus aureus From Raw and Spontaneously Fermented Camel Milk

Njage

2013

EJNFS

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“…Polymorphisms of the coa gene have been reported in S. aureus from different sources and animal species. 1,7…”

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confidence: 99%

Accuracy of PCR targeting different markers for Staphylococcus aureus identification: a comparative study using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as the gold standard

et al. 2017

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Staphylococcus aureus is considered a major pathogen in veterinary and human medicine, and the emergence of multidrug-resistant strains, such as livestock-associated methicillin-resistant S. aureus, means that reliable, inexpensive, and fast methods are required to identify S. aureus obtained from animal sources. We tested the accuracy of a PCR targeting the genes femA, nuc, and coa in identifying S. aureus from animals. A total of 157 Staphylococcus spp. isolates were examined by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry; 18 different Staphylococcus species were identified. Of 68 S. aureus isolates, the genes femA, nuc, and coa were found in 61, 53, and 32 isolates, respectively. Considering MALDI-TOF as the gold standard, the PCR assays targeting all 3 genes showed 100% specificity; the sensitivity values were 89.7, 77.9, and 47.0% for femA, nuc, and coa, respectively. Sensitivity was 100% when femA and nuc markers were targeted simultaneously. These results confirm PCR as an accurate method to identify S. aureus species from animal sources and strongly suggest the simultaneous use of primers targeting femA and nuc genes.

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“…Of the isolated S. aureus, eight strains (72.7%) were identified as enterotoxigenic. Previous studies from different parts of the world have reported enterotoxigenic S. aureus isolation rates ranging from 29% to 54% (Cardoso et al, 1999;Cenci-Goga et al, 2003;Kenny et al, 1993;Matsunaga et al, 1993;Riva et al, 2015;Stephan et al, 1999), while Aarestrup et al (1995) and Wang et al (2009) did not detect any enterotoxigenic S. aureus isolates among 160 isolates from bovine mastitis milk. Different enterotoxin genes can be attributed to isolation variations and sources (Hamidi et al, 2015;Valizadeh & Amini, 2016).…”

Section: Discussionmentioning

confidence: 87%

“…Previous studies from different parts of the world have reported enterotoxigenic S. aureus isolation rates ranging from 29% to 54% (Cardoso et al., 1999; Cenci‐Goga et al., 2003; Kenny et al., 1993; Matsunaga et al., 1993; Riva et al., 2015; Stephan et al., 1999), while Aarestrup et al. (1995) and Wang et al. (2009) did not detect any enterotoxigenic S. aureus isolates among 160 isolates from bovine mastitis milk.…”

Section: Discussionmentioning

confidence: 98%

Rapid detection of major enterotoxin genes and antibiotic resistance of Staphylococcus aureus isolated from raw milk in the Yazd province, Iran

Forouzani‐Moghaddam,

Habibi,

Hosseini‐Safa

et al. 2024

Veterinary Medicine & Sci

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IntroductionRaw milk is a nutrient‐rich food, but it may harbour harmful bacteria, such as enterotoxigenic Staphylococcus aureus (S. aureus), which can cause staphylococcal food poisoning. Antibiotic resistance of S. aureus in raw milk can increase the risk of such infections, particularly among susceptible individuals.ObjectiveThis study aimed to investigate the prevalence of enterotoxin genes a, d, g, i and j and the antibiotic resistance of S. aureus isolated from raw milk samples.MethodsDuring a 6‐month sampling period, 60 raw milk specimens were obtained from diverse locations in Yazd province, Iran. Antibiogram profiling was conducted via the disc diffusion method. In addition, staphylococcal enterotoxin (SE) genes a, d, g, i, and j were detected through real‐time PCR analysis.ResultsBacteriological assays confirmed the presence of S. aureus in 11 samples (18.3%). All isolates demonstrated 100% resistance to penicillin G but exhibited sensitivity to vancomycin, while resistance to other antibiotics ranged from 36.4% to 45.5%. The prevalence of enterotoxin genes in these strains showed variable distribution, with sea being the predominant SE (45.5%), followed by sed (36.4%), seg (18.2), sej and sei (9.1% each).ConclusionsThis study discovered the presence of multiple enterotoxins in S. aureus strains obtained from raw milk samples. These strains also demonstrated resistance to a variety of antibiotics. Since enterotoxigenic S. aureus is known to cause human food poisoning, monitoring food hygiene practices, especially during raw milk production, is critical.

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Lack of Staphylococcal Enterotoxin Production among Strains of Staphylococcus aureus from Bovine Mastitis in Denmark

Cited by 16 publications

References 12 publications

Phenotypic and Genotypic Antibiotic Resistance Patterns of Staphylococcus aureus From Raw and Spontaneously Fermented Camel Milk

Phenotypic and Genotypic Antibiotic Resistance Patterns of Staphylococcus aureus From Raw and Spontaneously Fermented Camel Milk

Accuracy of PCR targeting different markers for Staphylococcus aureus identification: a comparative study using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as the gold standard

Rapid detection of major enterotoxin genes and antibiotic resistance of Staphylococcus aureus isolated from raw milk in the Yazd province, Iran

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