Lack of SMALL ACIDIC PROTEIN 1 (SMAP1) causes increased sensitivity to an inhibitor of RUB/NEDD8-activating enzyme inArabidopsisseedlings

Abstract

SMALL ACIDIC PROTEIN 1 (SMAP1) functions upstream of the degradation of AUX/IAA-proteins in the response to 2,4-dichlorophenoxyacetic acid and physically interacts with the COP9 SIGNALOSOME (CSN). Also, its function is linked to RELATED TO UBIQUITIN (RUB) modification. To further investigate the relationship between SMAP1 and the RUB modification system, we examined the effect of MLN4924, an inhibitor of RUB/NEDD8-activating E1 enzyme, on the growth of Arabidopsis thaliana. We found that the anti-auxin resistant 1 mutants, which lack SMAP1, are more sensitive to MLN4924 than wild type and that SMAP1 is responsible for this hypersensitivity. This new evidence supports our previous speculation that SMAP1 acts in Cullin-RING ubiquitin E3 ligase regulated signaling processes via its interaction with components associated with the RUB modification system.

Proteomic Quantification of Lysine Acetylation and their Essential Role in Response to Pig Fed Different Protein Levels

“…In this research, we found that the acetylated JADE2 protein was involved in spliceosome and Fatty acid elongation pathway; BHLHB9, plays an important function in the control of cellular aging and survival, its simple name was p60TRP and its expression will benefit for cardiovascular functions (Mishra et al, 2012). In this research, its acelytation was benefit for cell survival or mRNA surveillance pathway; SP3 was one kind of transcription factor, which modulated the transcription activity of some genes (Xu et al, 2017), its acelytation was involved in cell survival; SMAP, one kind of small acidic protein, its function was not clearly in animals (Gong et al, 1997), but in plants, SMAP is very important for young plant growth and development (Oono and Nakasone, 2013). SECISBP2L was one figment of Selenoproteins.…”