Lack of retrovirus gene expression in teratocarcinoma stem cells is limited to the nucleus

Abstract

Experiments are described showing that the block to retrovirus replication by undifferentiated embryonal carcinoma cells can be overcome by fusion with differentiated cell karyoplasts but not cytoplasts. This supports previous notions that the expression of input proviral genes in teratocarcinoma stem cells is under different controls than in differentiated cells and indicates that the exercising of this control is limited to the nucleus of the embryonal carcinoma cell.

Embryonic Stem Cells: Introducing Exogenous Regulators into Embryonic Stem Cells

“…Additionally, studies on proteins and carbohydrates have been performed to characterize the EC cells and their induced descendants (Amano et al, 2010;Andrews et al, 1984;Jemmerson et al, 1985). The role of the extracellular matrix is also examined during differentiation induction of EC cells (Grabel, 1984;Wartiiovaara et al, 1984) as the karyoplasm in EC cell gene expression (Gautsch, 1982). Differentiation induction of EC cells has been successful with various substances, such as retinoic acid, dibutyryl cyclic AMP (Jones-Villeneuve et al, 1983;Muramatsu and Muramatsu, 1983), dimethyl sulfoxide (Edwards et al, 1983) and coculture with specific cell types (Allin, 1984).…”