Lack of Prolactin (PRL) mRNA Expression in Estrogen-treated Rat Mammary Glands.

Iwasaka, Toshiki; Umemura, Shinobu; Miyajima, Katsuhiro; Shoda, Toshiyuki; Takahashi, Akiko; Kakimoto, Kochi; Koizumi, Haruko; Miyakawa, Yoshifumi; Osamura, Yoshiyuki

doi:10.1293/tox.13.151

Cited by 2 publications

(2 citation statements)

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“…The significance of local extrapituitary PRL in rat mammary gland has not been elucidated, without suggestion of an autocrine/paracrine manner [43][44][45][46][47][48] . However, no PRL mRNA in rat mammary gland was detected following exposure to estrogen, in spite that the serum PRL levels and morphological change were similar to those in lactating mammary gland 49,50 . In addition, in hypophysectomized lactating rats, PRL mRNA was detected on the predominant involution of the mammary gland, in spite of extremely reduced serum PRL levels (Fig.…”

Section: Caution In the Assessment Of Rat Mammary Glandmentioning

confidence: 72%

Endocrine System and Endocrine Disrupting Chemicals(EDCs).

2001

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Abstract:Recently, much has been focused on the potential for a wide range of chemicals to interact with the disruption of the endocrine system in humans and animals. Some of those chemicals have been implicated as endocrine disrupting chemicals (EDCs). We represent this review that the current endocrine systems including of molecular biology and effects of EDCs are discussed, as examples pituitary and mammary gland. Alterations to the endocrine environment caused by EDCs may occur through interference with the synthesis, secretion, transport, binding, action, or elimination of natural hormones responsible for vital homeostasis, differentiation, development, reproduction, and/or behavior. It is important consideration for assessing the adverse effects of EDCs to have an understanding of normal endocrine systems, subsequently, dose, duration of exposure, age, sex, situation, and frequency are estimated. (J Toxicol Pathol 2001; 14: 59-64)

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Section: Caution In the Assessment Of Rat Mammary Glandmentioning

confidence: 72%

Endocrine System and Endocrine Disrupting Chemicals(EDCs).

2001

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“…Detection of HGF and TGF-β mRNA was carried out using a two-step in situ RT-PCR procedure as previously reported (Lee et al 1993; McMillan et al 1996; Nuovo 1996, 1997; Iwasaka et al 2000; Sinigalliano et al 2001) with the following modifications. Sections were pretreated with 10 μg/ml proteinase K (Sigma Aldrich; St Louis, MO) in 0.01 M PBS, pH 7.4, at RT for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Expression of Hepatocyte Growth Factor mRNA in Rat Liver Cirrhosis Induced byN-nitrosodimethylamine as Evidenced by In Situ RT-PCR

Hata

Ikeda

Uno

et al. 2002

J Histochem Cytochem.

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Hepatocyte growth factor (HGF) is a potent inducer of hepatocyte proliferation and is expressed during liver failure. In this study we used the in situ reverse transcriptase-polymerase chain reaction (RT-PCR) method to detect HGF mRNA expression in normal rat livers and cirrhotic rat livers induced by treatment with N-nitrosodimethylamine (DMN). In normal control livers, in situ RT-PCR detected HGF mRNA expression in Ito cells and Kupffer cells, both of which showed rounded morphologies. However, in the cirrhotic livers induced by DMN, HGF mRNA-positive cells were spindle-shaped and surrounded the hepatocytes located around the sinusoids. These cells appeared to be sinusoidal endothelial cells as well as Ito and Kupffer cells. Because it has been suggested that HGF expression is related to transforming growth factor-beta (TGF-beta) levels that may play an essential role in disease progression in cirrhotic livers, TGF-beta mRNA expression in normal and cirrhotic livers was also compared using in situ RT-PCR. Our results confirmed that expression of TGF-beta mRNA co-localized with HGF mRNA expression in the cirrhotic liver.

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Lack of Prolactin (PRL) mRNA Expression in Estrogen-treated Rat Mammary Glands.

Cited by 2 publications

References 39 publications

Endocrine System and Endocrine Disrupting Chemicals(EDCs).

Endocrine System and Endocrine Disrupting Chemicals(EDCs).

Expression of Hepatocyte Growth Factor mRNA in Rat Liver Cirrhosis Induced byN-nitrosodimethylamine as Evidenced by In Situ RT-PCR

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