Lack of MERS Coronavirus Neutralizing Antibodies in Humans, Eastern Province, Saudi Arabia

Gierer, Stefanie; Hofmann-Winkler, Heike; Albuali, Waleed H.; Bertram, Stephanie; Al-Rubaish, Abdullah M.; Yousef, Abdullah A; Al-Nafaie, Awatif N.; Al-Ali, Amein K.; Obeid, Obeid E.; Alkharsah, Khaled R.; Pöhlmann, Stefan

doi:10.3201/eid1912.130701

Cited by 46 publications

(37 citation statements)

References 9 publications

(8 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In three earlier cross-sectional serologic studies, none of the 3024 participants showed evidence of previous infection with MERS-CoV. 1,14,15 In our study, the detection of antibodies was most likely due to our focus on household contacts of index patients. Differences in assay sensitivity may play an additional role.…”

Section: Discussionmentioning

confidence: 67%

Transmission of MERS-Coronavirus in Household Contacts

et al. 2014

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 67%

Transmission of MERS-Coronavirus in Household Contacts

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The cIFA in this study produced a significant number of false positives that required exclusion by confirmatory neutralization assay (Aburizaiza et al, 2013). Another study conducted in Saudi Arabia made use of MERS-S pseudotyped lentiviruses to test for virus-neutralizing antibodies (Gierer et al, 2013b). HCoV-NL63 S pseudotyped viruses were used as controls.…”

Section: Diagnostics For Newly Emerged Mers-covmentioning

confidence: 99%

Serological assays for emerging coronaviruses: Challenges and pitfalls

2014

View full text Add to dashboard Cite

More than a decade after the emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) in 2002/2003 the occurrence of a novel CoV termed Middle East respiratory syndrome (MERS) CoV challenges researchers and public health authorities. To control spread and finally contain novel viruses, rapid identification and subsequent isolation of infected individuals and their contacts is of utmost importance. Next to methods for nucleic acid detection, validated serological assays are particularly important as the timeframe for antibody detection is less restricted. During the SARS-CoV epidemic a wide variety of serological diagnostic assays were established using multiple methods as well as different viral antigens. Even though the majority of the developed assays showed high sensitivity and specificity, numerous studies reported on cross-reactive antibodies to antigens from wide-spread common cold associated CoVs. In order to improve preparedness and responsiveness during future outbreaks of novel CoVs, information and problems regarding serological diagnosis that occurred during the SARS-CoV should be acknowledged. In this review we summarize the performance of different serological assays as well as the applicability of the two main applied antigens (spike and nucleocapsid protein) used during the SARS-CoV outbreak. We highlight challenges and potential pitfalls that occur when dealing with a novel emerging coronavirus like MERS-CoV. In addition we describe problems that might occur when animal sera are tested in serological assays for the identification of putative reservoirs. Finally, we give a recommendation for a serological testing scheme and outline necessary improvements that should be implemented for a better preparedness.

show abstract

“…MERS-CoV enfeksiyonu yaşlı-larda ve altta yatan başka hastalığı olanlarda daha ağır klinik seyir ve ölüme yol açmaktadır (1) . Suudi Arabistan'da yürütülen serolojik çalışmalarda MERS-CoV antikorlarının prevalansı düşüktür (39,40) . Suudi Arabistanlı genel popülasyonu temsil eden (10.009 kişi) bir çalışmada, seropozitiflik oranı genel popülas-yonda %0,15, deve çobanları ve kesimevi çalışanlarında %3,1 bulunmuştur (41) .…”

Section: Mers-cov Için Başlıca Hayvan Ara Konağın Arapunclassified

Middle East Respiratory Syndrome-Coronavirus (MERSCoV) Infection

İnal¹

2016

Okmeydani Medical Journal

View full text Add to dashboard Cite

show abstract

Lack of MERS Coronavirus Neutralizing Antibodies in Humans, Eastern Province, Saudi Arabia

Cited by 46 publications

References 9 publications

Transmission of MERS-Coronavirus in Household Contacts

Transmission of MERS-Coronavirus in Household Contacts

Serological assays for emerging coronaviruses: Challenges and pitfalls

Middle East Respiratory Syndrome-Coronavirus (MERSCoV) Infection

Contact Info

Product

Resources

About