Lack of interferon response in animals to naked siRNAs

Abstract

Supplementary Figure 1 RNase digestion of poly(I:C) and siRNA. RNase degradation of poly(I:C). 1 µg poly(I:C) was incubated with RNase (at indicated concentrations from 0-50 µg/mL) at 37 o C for 30 min, then electrophoresed on a 1% (wt/vol) agarose gel.

RNA interference as a key to knockdown overexpressed cyclooxygenase-2 gene in tumour cells

“…On the contrary, other findings demonstrate that is even possible to administer naked, synthetic siRNA to mice without inducing any interferon response (Heidel et al, 2004). In our experimental model, we analysed the phosphorylation status (on Tyr701) of STAT-1 protein as a marker of the interferon system activation (Brierley and Fish, 2005).…”

Critical role of acidic sphingomyelinase in murine hepatic ischemia-reperfusion injury

“…4C), consistent with recent reports using naked siRNAs. 37 Next, mice were injected into the tail 2 consecutive days with either ASMase-siRNA or scrambled siRNA and then subjected to I/R the day after the last dose. ASMase-siRNA decreased hepatic ASMase mRNA levels (Fig.…”

Therapeutic silencing of mutant huntingtin with siRNA attenuates striatal and cortical neuropathology and behavioral deficits

“…siRNA has been administered into cerebroventricles, vasculature, intrathecal space, and parenchyma (16)(17)(18)(19)(20). siRNAs were found effective and safe when introduced into mice and non-human primates (19,21,22). Several limitations impede progress in using siRNAs as a treatment for HD: entry and effectiveness in adult neurons without the use of potentially toxic transfection reagents; a clear demonstration that gene silencing reduces protein expression; and an improvement in behavioral deficits and neuropathology, especially neuron survival.…”