Lack of interference of common phytoecdysteroids with production of nitric oxide by immune-activated mammalian macrophages

Effects of 20-hydroxyecdysone, Leuzea carthamoides extracts, dexamethasone and their combinations on the NF-κB activation in HeLa cells

“…Doubts over the contribution of 20E to the anti‐inflammatory effects of Leuzea extracts were expressed by Harmata et al . (2008) [8] . Although 20E alone was an NF‐ κ B inhibitor in our test model, similar uncertainties arise over the contribution of 20E to the NF‐ κ B inhibition caused by Leuzea extracts for three reasons.…”

“…Anti‐inflammatory effects for 20E have been reported by some authors (as reviewed by Lafont and Dinan 2003), [5] but there are also reports that could not confirm such effects in vivo [7] . Harmatha et al [8] . could not detect reduced NO production of peritoneal macrophages upon LPS or interferone stimulation, even after incubation with up to 100 µ m 20E.…”

“…Beside the ecdysteroids 20E, makisterone C, polypodin B and ajugasterone C [37,38] and flavonoids, [39,40] more attention may be given to sesquiterpene lactones, [28,29] tiophene polyacetylenes, [41,42] and stilbenes, [43] which are known for their cytotoxic and anti‐inflammatory properties in different models involving NF‐ κ B activity modulation (see also review by Kokoska and Janovska [11] ). N‐feruloylserotonins have also already been suggested [8] . The diverse activities of constituent groups on single physiological targets may cause unpredictable experimental effects when the whole mixtures with distinctive proportions of those groups are tested.…”

20-Hydroxyecdysone decreases weight and hyperglycemia in a diet-induced obesity mice model

“…Compounds similar to 20HE counteract insulin-dependent growth in Drosophila (8); however, we did not observe any effect of 20HE administration on animal growth or body length. It is also know that ecdysone coordinates optic lobe neurogenesis in Drosophila via a nitric oxide signaling pathway; however, nitric oxide-mediated effects in animals have not been confirmed for 20HE (14,19,20).…”

Phytoecdysteroids: A Novel, Non-Androgenic Alternative for Muscle Health and Performance

“…Likewise, carrageenan is a known pro-inflammatory agent which negatively affects muscle strength [36]. Ochieng et al have noted a powerful inhibitory effect of isolated PEs on carrageenan-induced inflammation [33], but others have suggested that some PEs do not have anti-inflammatory properties [37,38]. While these results are not definitive proof that PEs can modulate skeletal muscle damage due to inflammation, further research is certainly warranted to explore the potential therapeutic potential of PEs to modulate skeletal muscle inflammation and enhance regeneration.…”