Lack of infection of vertebrate cells by the densovirus from the maize worm Mythimna loreyi (MlDNV)

El-Far, Mohamed; Li, Y.; Fédière, Gilles; Abol-Ela, S.; Tijssen, Peter

doi:10.1016/j.virusres.2003.09.010

Cited by 23 publications

(12 citation statements)

References 20 publications

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“…Densoviruses can infect a wide range of invertebrates including many insects as well as crustaceans and echinoderms such as starfish and sea urchins [58,59]. Some densoviruses have been used to inoculate vertebrate cells unsuccessfully [60,61]. In contrast a densovirus genome was detected by metagenomics and PCR confirmed in a human cerebral spinal fluid from an unexplained case of encephalitis [62].…”

Section: Plos Onementioning

confidence: 99%

Plasma virome of 781 Brazilians with unexplained symptoms of arbovirus infection include a novel parvovirus and densovirus

et al. 2020

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Plasma from patients with dengue-like symptoms was collected in 2013 to 2016 from the Brazilian states of Tocantins and Amapa. 781 samples testing negative for IgM against Dengue, Zika, and Chikungunya viruses and for flaviviruses, alphaviruses and enteroviruses RNA using RT-PCRs were analyzed using viral metagenomics. Viral particles-associated nucleic acids were enriched, randomly amplified, and deep sequenced in 102 mini-pools generating over 2 billion reads. Sequence data was analyzed for the presence of known and novel eukaryotic viral reads. Anelloviruses were detected in 80%, human pegivirus 1 in 19%, and parvovirus B19 in 17% of plasma pools. HIV and enteroviruses were detected in two pools each. Previously uncharacterized viral genomes were also identified, and their presence in single plasma samples confirmed by PCR. Chapparvovirus and ambidensovirus genomes, both in the Parvoviridae family, were partially characterized showing 33% and 34% identity in their NS1 sequences to their closest relative. Molecular surveillance using pre-existing plasma from febrile patients provides a readily scalable approach for the detection of novel, potentially emerging, viruses.

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Section: Plos Onementioning

confidence: 99%

Plasma virome of 781 Brazilians with unexplained symptoms of arbovirus infection include a novel parvovirus and densovirus

et al. 2020

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“…DNVs are found in a wide range of insect families and can cause acute diseases that are often fatal for their insect host (Barreau et al, 1996;Mutuel et al, 2010;Suto, 1979;Szelei et al, 2011). As DNVs are not known to infect mammals (Dupont, 2003;El-Far et al, 2004), biopesticides derived from engineered or natural DNVs have been developed to reduce lepidopteran, mosquito and cockroach populations (Buchatskiȋ et al, 1987;Carlson et al, 2006;Hu et al, 2000;Jiang et al, 2007;Monsarrat et al, 1984;Tal & Attathom, 1993). However, some DNVs may only induce sublethal infection and may even confer a beneficial effect on their host (i) by increasing the production of the winged morph and therefore promoting insect dispersal, or (ii) by protecting against pathogenic baculovirus infection (Ryabov et al, 2009;van Munster et al, 2003a;Xu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Transcription of densovirus endogenous sequences in the Myzus persicae genome

Clavijo

Munster

Monsion

et al. 2016

Journal of General Virology

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Integration of non-retroviral sequences in the genome of different organisms has been observed and, in some cases, a relationship of these integrations with immunity has been established. The genome of the green peach aphid, Myzus persicae (clone G006), was screened for densovirus-like sequence (DLS) integrations. A total of 21 DLSs localized on 10 scaffolds were retrieved that mostly shared sequence identity with two aphid-infecting viruses, Myzus persicae densovirus (MpDNV) and Dysaphis plantaginea densovirus (DplDNV). In some cases, uninterrupted potential ORFs corresponding to non-structural viral proteins or capsid proteins were found within DLSs identified in the aphid genome. In particular, one scaffold harboured a complete virus-like genome, while another scaffold contained two virus-like genomes in reverse orientation. Remarkably, transcription of some of these ORFs was observed in M. persicae, suggesting a biological effect of these viral integrations. In contrast to most of the other densoviruses identified so far that induce acute host infection, it has been reported previously that MpDNV has only a minor effect on M. persicae fitness, while DplDNV can even have a beneficial effect on its aphid host. This suggests that DLS integration in the M. persicae genome may be responsible for the latency of MpDNV infection in the aphid host.

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“…Similar to other parvovirus, densoviruses (DNVs) are small icosahedral, no-enveloped particles of 18-26 nm in diameter and their genomes consist of a 4-6.5 kb single-stranded linear DNA equimolecularly encapsidated as plus and minus strands in separate virions. DNVs can infect a wide range of insects including Lepidoptera, Diptera, Orthoptera and Dictyoptrea [2] and lead to death of the host in most cases, but they are not able to infect tissues of vertebrates [3], thus making them potential biological agents for controlling main agricultural pests [4,5].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the genome structure of Bombyx mori densovirus (China isolate)

et al. 2006

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The genome of Bombyx mori densovirus (China isolate), termed as BmDNV-3, is composed of two kinds of different single-stranded linear DNA molecules (VD1 and VD2). In this study, the viral DNA molecules were purified and cloned into pUC119 vector, and the complete nucleotide sequence was determined. Sequence analysis showed that VD1 genome consisted of 6,543 nts including inverted terminal repeats (ITRs) of 224 nts, and VD2 genome consisted of 6,022 nts including ITRs of 524 nts. Comparison of the complete genome sequence between BmDNV-3 and BmDNV-2 (Yamanashi isolate) showed an identity of 98.4% in VD1 and 97.7% in VD2, with a total number of 228 bp substitutions, 11 bp deletions and 3 bp insertions found in BmDNV-3. A single nucleotide ''A'' deletion at nt 1589 in BmDNV-3 caused a frame shift mutation and brought about a premature stop codon, thus dividing VD2 of BmDNV-3 into two ORFs (named VD2 ORF1a and VD2 ORF1b) within that region, while there was only one ORF (named VD2 ORF1) in the corresponding region of BmDNV-2 (Yamanashi isolate). Comparative polymorphisms of ORFs and ITR regions of the two viral genomes showed that highly variable regions were mainly located in VD1 ORF3, VD1 ORF4, VD2 ORF2, and ITRs of BmDNV-3. Northern blots analysis revealed that VD1 had 1.1 kb and 1.5 kb transcripts from the left half of its plus strand, and one transcript about 3.3 kb from the right half of its minus strand. Sequencing of 3¢ and 5¢ RACE products showed that the 1.1 kb transcript started at nt 290 and ended at nt 1437, the 1.5 kb transcript started at nt 1423 and ended at nt 2931, and the 3.3 kb transcript started at nt 6287 and ended at nt 2922. These results help us to further understand the variation between different DNV genera and its possible causes, providing clues for studying the evolutionary history of densoviruses.

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Lack of infection of vertebrate cells by the densovirus from the maize worm Mythimna loreyi (MlDNV)

Cited by 23 publications

References 20 publications

Plasma virome of 781 Brazilians with unexplained symptoms of arbovirus infection include a novel parvovirus and densovirus

Plasma virome of 781 Brazilians with unexplained symptoms of arbovirus infection include a novel parvovirus and densovirus

Transcription of densovirus endogenous sequences in the Myzus persicae genome

Characterization of the genome structure of Bombyx mori densovirus (China isolate)

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