Lack of FIBRILLIN6 in <i>Arabidopsis thaliana</i> affects light acclimation and sulfate metabolism

Lee, Kwanuk; Lehmann, Martin; Paul, Melanie; Wang, Liangsheng; Luckner, Manja; Wanner, Gerhard; Geigenberger, Peter; Leister, Dario; Kleine, Tatjana

doi:10.1111/nph.16246

Cited by 20 publications

(18 citation statements)

References 87 publications

(201 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The techniques employed to accomplish our goals include the mining of public databases in order to identify co‐regulated factors (guilt‐by‐association approaches), genetic screens, environmental perturbation experiments combined with ‘omics’ technologies (transcriptomics, proteomics and metabolomics) and the analysis of molecular and gene regulatory networks. For example, FIBRILLIN6 (FBN6) (Table 2) was identified as a factor involved in high light acclimation and cadmium tolerance via a guilt‐by‐association approach (Lee et al ., 2020). Moreover, a screen for mutants that can cope better than WT with lincomycin, an inhibitor of organellar translation, revealed that a disturbance in the secondary cell wall promotes early chloroplast development (Xu et al ., 2020a).…”

Section: Ra3 Signalling: Coordination Of Different Acclimation Respomentioning

confidence: 99%

Acclimation in plants – the Green Hub consortium

et al. 2021

Self Cite

View full text Add to dashboard Cite

Summary Acclimation is the capacity to adapt to environmental changes within the lifetime of an individual. This ability allows plants to cope with the continuous variation in ambient conditions to which they are exposed as sessile organisms. Because environmental changes and extremes are becoming even more pronounced due to the current period of climate change, enhancing the efficacy of plant acclimation is a promising strategy for mitigating the consequences of global warming on crop yields. At the cellular level, the chloroplast plays a central role in many acclimation responses, acting both as a sensor of environmental change and as a target of cellular acclimation responses. In this Perspective article, we outline the activities of the Green Hub consortium funded by the German Science Foundation. The main aim of this research collaboration is to understand and strategically modify the cellular networks that mediate plant acclimation to adverse environments, employing Arabidopsis, tobacco (Nicotiana tabacum) and Chlamydomonas as model organisms. These efforts will contribute to ‘smart breeding’ methods designed to create crop plants with improved acclimation properties. To this end, the model oilseed crop Camelina sativa is being used to test modulators of acclimation for their potential to enhance crop yield under adverse environmental conditions. Here we highlight the current state of research on the role of gene expression, metabolism and signalling in acclimation, with a focus on chloroplast‐related processes. In addition, further approaches to uncovering acclimation mechanisms derived from systems and computational biology, as well as adaptive laboratory evolution with photosynthetic microbes, are highlighted.

show abstract

Section: Ra3 Signalling: Coordination Of Different Acclimation Respomentioning

confidence: 99%

Acclimation in plants – the Green Hub consortium

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…The Web MicroRNA Designer program (http://wmd3.weigelworld.org/) was applied in order to generate two amiRNA constructs (amiR1 and amiR2) targeting different regions of the MTERF2 (AT2G21710) gene. The amiR constructs were generated, as described [30], while using the primers provided in Supplementary Table S1. The individual T1 seeds were selected by GFP fluorescence, and independent T3 and T4 transgenic lines were used for phenotypic analysis.…”

Section: Generation Of Amirna-mediated Knockdown Lines Gus and C-mycmentioning

confidence: 99%

Arabidopsis Mitochondrial Transcription Termination Factor mTERF2 Promotes Splicing of Group IIB Introns

Lee

Leister

Kleine

2021

Cells

Self Cite

View full text Add to dashboard Cite

Plastid gene expression (PGE) is essential for chloroplast biogenesis and function and, hence, for plant development. However, many aspects of PGE remain obscure due to the complexity of the process. A hallmark of nuclear-organellar coordination of gene expression is the emergence of nucleus-encoded protein families, including nucleic-acid binding proteins, during the evolution of the green plant lineage. One of these is the mitochondrial transcription termination factor (mTERF) family, the members of which regulate various steps in gene expression in chloroplasts and/or mitochondria. Here, we describe the molecular function of the chloroplast-localized mTERF2 in Arabidopsis thaliana. The complete loss of mTERF2 function results in embryo lethality, whereas directed, microRNA (amiR)-mediated knockdown of MTERF2 is associated with perturbed plant development and reduced chlorophyll content. Moreover, photosynthesis is impaired in amiR-mterf2 plants, as indicated by reduced levels of photosystem subunits, although the levels of the corresponding messenger RNAs are not affected. RNA immunoprecipitation followed by RNA sequencing (RIP-Seq) experiments, combined with whole-genome RNA-Seq, RNA gel-blot, and quantitative RT-PCR analyses, revealed that mTERF2 is required for the splicing of the group IIB introns of ycf3 (intron 1) and rps12.

show abstract

“…The RNAi of LeChrC ( FBN1 ) and mutants of fbi4 in apple ( Malus domestica ) and Arabidopsis were more sensitive to Botrytis cinerea, Erwinia amylovora and Pseudomonas syringae , respectively ( Leitner-Dagan et al, 2006 ; Singh et al, 2010 ). The fbn6 mutant of Arabidopsis showed resistance to cadmium and high-light stresses ( Lee et al, 2020 ). The expression of jasmonic acid (JA) synthesis related genes in fbn5 mutants were inhibited under high-light stress ( Otsubo et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and expression analysis of fibrillin (FBN) gene family in tomato (Solanum lycopersicum L.)

Sun

Min

Zhang

2022

PeerJ

View full text Add to dashboard Cite

Background Fibrillin (FBN) proteins are widely distributed in the photosynthetic organs. The members of FBN gene family play important roles in plant growth and development, and response to hormone and stresses. Tomato is a vegetable crop with significantly economic value and model plant commonly used in research. However, the FBN family has not been systematical studied in tomato. Methods In this study, 14 FBN genes were identified in tomato genome by Pfam and Hmmer 3.0 software. ExPASy, MEGA 6.0, MEME, GSDS, TBtools, PlantCARE and so on were used for physical and chemical properties analysis, phylogenetic analysis, gene structure and conserved motifs analysis, collinearity analysis and cis-acting element analysis of FBN family genes in tomato. Expression characteristics of SlFBNs in different tissues, fruit shape near isogenic lines (NILs), Pst DC3000 and ABA treatments were analyzed based on transcriptome data and quantitative Real-time qPCR (qRT-PCR) analysis. Results The SlFBN family was divided into 11 subgroups. There were 8 FBN homologous gene pairs between tomato and Arabidopsis. All the members of SlFBN family contained PAP conserved domain, but their gene structure and conserved motifs showed apparent differences. The cis-acting elements of light and hormone (especially ethylene, methyl jasmonate (MeJA) and abscisic acid (ABA)) were widely distributed in the SlFBN promoter regions. The expression analysis found that most of SlFBNs were predominantly expressed in leaves of Heinz and S. pimpinellifolium LA1589, and showed higher expressions in mature or senescent leaves than in young leaves. Expression analysis of different tissues and fruit shape NILs indicated SlFBN1, SlFBN2b and SlFBN7a might play important roles during tomato fruit differentiation. All of the SlFBNs responded to Pst DC3000 and ABA treatments. The results of this study contribute to exploring the functions and molecular mechanisms of SlFBNs in leaf development, fruit differentiation, stress and hormone responses.

show abstract

Lack of FIBRILLIN6 in Arabidopsis thaliana affects light acclimation and sulfate metabolism

Cited by 20 publications

References 87 publications

Acclimation in plants – the Green Hub consortium

Acclimation in plants – the Green Hub consortium

Arabidopsis Mitochondrial Transcription Termination Factor mTERF2 Promotes Splicing of Group IIB Introns

Genome-wide identification and expression analysis of fibrillin (FBN) gene family in tomato (Solanum lycopersicum L.)

Contact Info

Product

Resources

About