Lack of extracellular slime effect on treatment outcome of <i>Staphylococcus epidermidis</i> experimental endocarditis

Steckelberg, James M.; Keating, Michael R.; Rouse, Mark S.; Wilson, Wildiani

doi:10.1093/jac/23.1.117

Cited by 5 publications

(4 citation statements)

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“…Our experiments using isogenic strains of S. epidermidis that grossly differ in levels of slime production in vitro allow a more direct determination of the contribution of slime production to virulence in vivo. Our findings agree with those of Stekelberg et al, who described no effect of slime production on the treatment of experimental S. epidermidis endocarditis (25). The results differ from those of a subcutaneous catheter infection model and an intraperitoneal infection model with mice, in which the importance of extracellular slime in establishing infection was shown (5,16).…”

Section: Discussionsupporting

confidence: 83%

The Abilities of aStaphylococcus epidermidisWild-Type Strain and Its Slime-Negative Mutant To Induce Endocarditis in Rabbits Are Comparable

et al. 1998

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The abilities of a parent and mutant pair of Staphylococcus epidermidis strains, the slime-producing parent RP62A and its slime-negative mutant, to establish endocarditis in a rabbit model of aortic valve endocarditis and to accumulate and adhere to surfaces in vitro were compared. Vegetation titer and infection rate depended on the presence or absence of a catheter (P = 0.020) and on inoculum size (P < 0.001) but not on the infecting strain. The ability of the parent strain vis-à-vis its mutant to accumulate in vitro on surfaces as demonstrated in a slime test did not correlate with any enhancement in the development of endocarditis in the rabbit model. In vitro initial adherence rates were identical. Both isolates accumulated to the same reduced extent in vitro in the presence of serum, albumin, or gelatin. Adhesion was equally promoted by addition of fibronectin. These data suggest that the in vitro phenomenon of accumulation described as slime production in the absence of serum may not be an important virulence determinant in vivo.

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Section: Discussionsupporting

confidence: 83%

The Abilities of aStaphylococcus epidermidisWild-Type Strain and Its Slime-Negative Mutant To Induce Endocarditis in Rabbits Are Comparable

et al. 1998

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“…epidermidis strains to produce slime in ritro was not found to affect the outcome of vancomycin treatment in an experimental catheterinduced endocarditis in rabbits. 33 The evidence suggesting that hydrophobicity is important in the adherence of CNS is not conclusive and different measurements of hydrophobicity may give conflicting results.34 Nevertheless, it is curious that although about half of the endocarditis strains were hydrophobic, all of the line-associated strains were hydrophilic, a result that must reflect differences in their cell-wall structure.…”

Section: Discussionmentioning

confidence: 99%

Adherence characteristics of coagulase-negative staphylococci isolated from patients with infective endocarditis

Cree

Phillips

Noble

1995

Journal of Medical Microbiology

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Summary. Coagulase-negative staphylococci isolated from patients with endocarditis were divided according to whether the infection was of native or of prosthetic valves and was acquired either in the community or in hospital. Comparisons were made with strains from intravenous line-associated bacteraemias. All strains were examined by direct and indirect adherence tests. Line-associated bacteraemia strains were more likely to produce slime and were more hydrophilic but were less likely to attach HEp2 tissue culture cells than were endocarditis strains, and almost equally likely to adhere to plastic and extracellular matrix proteins. Amongst the endocarditis strains, there was little difference in slime production but hospital-acquired or prosthetic-valve strains were more hydrophobic and more likely to adhere to silicone than were the native-valve or community-acquired strains. Exposure of extracellular matrix proteins on native valves due to a pre-existing non-infective heart condition may account for the selection of strains able to adhere to fibronectin or laminin.

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“…It has been suggested that the decreased antibiotic susceptibility of attached bacteria may result from the glycocalyx providing a barrier that reduces penetration of antibiotics. However, this view is not supported by the finding that diffusion to the bacterial surface is only minimally retarded in biofilms (Steckelberg et al, 1989;Dunne et al, 1993). Slow growth is characteristic of many infections (Zak & Sande, 1981) and of bacteria growing within a biofilm on prosthetic devices (Brown et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

The effects of adherence to silicone surfaces on antibiotic susceptibility in Staphylococcus aureus

et al. 1997

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Sensitivity of Staphylococcus aureus to the antibiotics tetracycline, benzylpenicillin and vancomycin was found to decrease by 2-10-fold when cells were grown adherent to silicone catheter surfaces. Sensitivity to rifampicin and fusidic acid was not significantly altered in adherent cells. Susceptibility further decreased with increased adherence time prior to antibiotic challenge. The resistance observed was not genotypic, or due to the presence of a specialized subpopulation of bacteria, as it disappeared when the bacteria were removed from the catheter, subcultured and retested. Also, adherent bacteria were found to grow more slowly than bacteria growing planktonically. It is concluded that the decrease in antibiotic susceptibility of adherent bacteria is a function of the physiological status of the individual cells rather than a function of biofilm formation or slime production. The decrease in growth rate of the adherent bacteria is a result of the adherence process rather than a result of nutrient depletion. The decrease in growth rate is implicated, but is not the sole factor, in the decreased antibiotic susceptibility of adherent bacteria.

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Lack of extracellular slime effect on treatment outcome of Staphylococcus epidermidis experimental endocarditis

Cited by 5 publications

References 0 publications

The Abilities of aStaphylococcus epidermidisWild-Type Strain and Its Slime-Negative Mutant To Induce Endocarditis in Rabbits Are Comparable

The Abilities of aStaphylococcus epidermidisWild-Type Strain and Its Slime-Negative Mutant To Induce Endocarditis in Rabbits Are Comparable

Adherence characteristics of coagulase-negative staphylococci isolated from patients with infective endocarditis

The effects of adherence to silicone surfaces on antibiotic susceptibility in Staphylococcus aureus

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