Lack of Evidence That Male Fetal Microchimerism is Present in Endometriosis

Fassbender, Amelie; Dębiec‐Rychter, Maria; Bree, Rieta Van; Vermeesch, Joris Robert; Meuleman, Christel; Tomassetti, Carla; Peeraer, Karen; D’Hooghe, Thomas; Lebovic, Dan I.

doi:10.1177/1933719115574343

Cited by 4 publications

(7 citation statements)

References 38 publications

(49 reference statements)

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“…In addition, our present data seem contradictory to most literature suggesting a significant association of microchimerism with most autoimmune diseases such as systemic sclerosis and other conditions mentioned above, whereas we did not observed higher frequencies of FMc in blood cell subpopulations of patients with psoriasis in comparison to controls. Moreover, no microchimeric cells in affected and unaffected skin were detected.…”

Section: Discussioncontrasting

confidence: 99%

“…did not detect any FMc in the tissue of lichen sclerosus of the vulva. Fassbender et al . did not find FMc in endometrium from either women with endometriosis or healthy women.…”

Section: Discussionmentioning

confidence: 88%

“…However, the numbers of patients and controls tested for this report are still low, and much larger samples are needed for a definitive result. It is worth noting, however, that other reports in this field are frequently based on similarly low numbers of cases (see for example Table of Fassbender et al …”

Section: Discussionmentioning

confidence: 98%

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Lack of detectable fetal microchimerism in psoriasis vulgaris lesions and in non‐affected skin in spite of its presence in peripheral bloodCD34‐positive andCD34‐negative cells

Niepiekło-Miniewska

Baran

Szepietowski

et al. 2016

Acad Dermatol Venereol

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Section: Discussioncontrasting

confidence: 99%

“…did not detect any FMc in the tissue of lichen sclerosus of the vulva. Fassbender et al . did not find FMc in endometrium from either women with endometriosis or healthy women.…”

Section: Discussionmentioning

confidence: 88%

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Lack of detectable fetal microchimerism in psoriasis vulgaris lesions and in non‐affected skin in spite of its presence in peripheral bloodCD34‐positive andCD34‐negative cells

Niepiekło-Miniewska

Baran

Szepietowski

et al. 2016

Acad Dermatol Venereol

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“…A panel of molecular biology tools including quantitative PCR, microarray expression, RNA-seq, and real time RT-PCR were employed to examine the Y chromosome microchimerism in the endometrium using secretory phase samples from fertile and infertile patients with severe OE (stage IV) and without endometriosis. This experimental strategy was adopted since earlier studies using FISH yielded conflicting results [14, 15]. FISH is a relatively less sensitive technique and it probes into a very small portion of the sample [22–25].…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, direct evidence of male microchimerism in endometriosis was earlier reported in a study [14]. Fassbender et al, however, failed to obtain evidence of such male microchimerism in the endometrium based on FISH-based experiments in a total of 31 patients, of which 19 had stage-confirmed endometriosis [15]. In the present study, we have examined this issue of Y-chromosomal microchimerism in fertile and infertile women with ovarian endometriosis (OE), without any reported uterine pathology.…”

Section: Introductionmentioning

confidence: 99%

Genomic evidence of Y chromosome microchimerism in the endometrium during endometriosis and in cases of infertility

Bhat

Sharma

Roy

et al. 2019

Reprod Biol Endocrinol

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Background Previous studies, which were primarily based on the fluorescent in-situ hybridisation (FISH) technique, revealed conflicting evidence regarding male foetal microchimerism in endometriosis. FISH is a relatively less sensitive technique, as it is performed on a small portion of the sample. Additionally, the probes used in the previous studies specifically detected centromeric and telomeric regions of Y chromosome, which are gene-sparse heterochromatised regions. In the present study, a panel of molecular biology tools such as qPCR, expression microarray, RNA-seq and qRT-PCR were employed to examine the Y chromosome microchimerism in the endometrium using secretory phase samples from fertile and infertile patients with severe (stage IV) ovarian endometriosis (OE) and without endometriosis. Methods Microarray expression analysis followed by validation using RNA-seq and qRT-PCR experiments at the RNA levels and further validation at the DNA level by qPCR of target inserts for selected targets in eutopic endometrium samples obtained from control (CON) and stage IV ovarian endometriosis (OE), either from fertile (FCON and FOE; n = 30/each) or infertile (ICON and IOE; n = 30/each) women, were performed. Results Six coding (AMELY, PCDH11, SRY, TGIF2LY, TSPY3, and USP9Y) and 10 non-coding (TTTY2, TTTY4C, TTTY5, TTTYY6, TTTY8, TTTY10, TTTY14, TTTY21, TTTY22, and TTTY23) genes exhibited a bimodal pattern of expression characterised by low expression in samples from fertile patients and high expression in samples from infertile patients. Seven coding MSY-linked genes (BAGE, CD24, EIF1AY, NLGN4Y, PRKY, VCY and ZFY) exhibited differential regulation in microarray analysis, and this change was validated by RNA-seq or qRT-PCR. DNA inserts for 7 genes in various samples were validated by qPCR. The prevalence and concentration of PCR-positive target inserts for BAGE, PRKY, TTTY9A and ZFY displayed higher values in the fertile, control (FCON) patients compared with the fertile, endometriosis patients (FOE). Conclusion Several coding and non-coding MSY-linked genes displayed microchimerism as evidenced by the presence of their respective DNA inserts, along with their differential transcript expression, in the endometrium during endometriosis and in cases of infertility. Electronic supplementary material The online version of this article (10.1186/s12958-019-0465-z) contains supplementary material, which is available to authorized users.

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Fetal microchimerism and maternal health: A review and evolutionary analysis of cooperation and conflict beyond the womb

et al. 2015

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Summary The presence of fetal cells has been associated with both positive and negative effects on maternal health. These paradoxical effects may be due to the fact that maternal and offspring fitness interests are aligned in certain domains and conflicting in others, which may have led to the evolution of fetal microchimeric phenotypes that can manipulate maternal tissues. We use cooperation and conflict theory to generate testable predictions about domains in which fetal microchimerism may enhance maternal health and those in which it may be detrimental. This framework suggests that fetal cells may function both to contribute to maternal somatic maintenance (e.g. wound healing) and to manipulate maternal physiology to enhance resource transmission to offspring (e.g., enhancing milk production). In this review, we use an evolutionary framework to make testable predictions about the role of fetal microchimerism in lactation, thyroid function, autoimmune disease, cancer and maternal emotional and psychological health.

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Lack of Evidence That Male Fetal Microchimerism is Present in Endometriosis

Abstract: We were unable to confirm our hypothesis that male fetal microchimerism is present in eutopic and/or ectopic EM obtained from women with endometriosis.

Cited by 4 publications

References 38 publications

Lack of detectable fetal microchimerism in psoriasis vulgaris lesions and in non‐affected skin in spite of its presence in peripheral bloodCD34‐positive andCD34‐negative cells

Lack of detectable fetal microchimerism in psoriasis vulgaris lesions and in non‐affected skin in spite of its presence in peripheral bloodCD34‐positive andCD34‐negative cells

Genomic evidence of Y chromosome microchimerism in the endometrium during endometriosis and in cases of infertility

Fetal microchimerism and maternal health: A review and evolutionary analysis of cooperation and conflict beyond the womb

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