Lack of evidence for increased adherent growth in broth or human serum of clinically significant coagulase-negative staphylococci

Wilcox, Mark H.; Schumacher-Perdreau, F.

doi:10.1016/0195-6701(94)90014-0

Cited by 12 publications

(4 citation statements)

References 41 publications

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“…This phenotypic resistance relates to a multilayered growth on surfaces and was observed despite similar initial levels of adherence on surfaces and similar levels of growth in suspension. The significance of adherent growth of coagulase-negative staphylococci has previously been assessed both in vitro and in an endocarditis model (20,27). The in vitro study considered various culture conditions that closely mimic the clinical situation with both important clinical isolates and isolates from the skin of volunteers (27).…”

Section: Discussionmentioning

confidence: 99%

“…The significance of adherent growth of coagulase-negative staphylococci has previously been assessed both in vitro and in an endocarditis model (20,27). The in vitro study considered various culture conditions that closely mimic the clinical situation with both important clinical isolates and isolates from the skin of volunteers (27). The in vivo study considered the pair of isogenic strains used in the present study (20).…”

Section: Discussionmentioning

confidence: 99%

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Impact of Bacterial Biofilm Formation on In Vitro and In Vivo Activities of Antibiotics

Schwank

Rajacic

Zimmerli

et al. 1998

Antimicrob Agents Chemother

157

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The impact of bacterial adherence on antibiotic activity was analyzed with two isogenic strains of Staphylococcus epidermidis that differ in the features of their in vitro biofilm formation. The eradication of bacteria adhering to glass beads by amikacin, levofloxacin, rifampin, or teicoplanin was studied in an animal model and in a pharmacokinetically matched in vitro model. The features of S. epidermidis RP62A that allowed it to grow on surfaces in multiple layers promoted phenotypic resistance to antibiotic treatment, whereas strain M7 failed to accumulate, despite initial adherence on surfaces and growth in suspension similar to those for RP62A. Biofilms of S. epidermidis M7 were better eradicated than those of strain RP62A in vitro (46 versus 31%;P < 0.05) as well as in the animal model (39 versus 9%; P < 0.01).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Impact of Bacterial Biofilm Formation on In Vitro and In Vivo Activities of Antibiotics

Schwank

Rajacic

Zimmerli

et al. 1998

Antimicrob Agents Chemother

157

View full text Add to dashboard Cite

show abstract

“…The subsequent coating of the microtiter plates with fibronectin over the serum layer enhanced S. epidermidis wild-type and mutant adherence to similar extents. Again, accumulation of the wild type was significantly reduced in the presence of serum in comparison to accumulation in the serum-free media (29). In the presence of serum, the wild type has no advantage over the slime-negative mutant.…”

Section: Discussionmentioning

confidence: 84%

The Abilities of aStaphylococcus epidermidisWild-Type Strain and Its Slime-Negative Mutant To Induce Endocarditis in Rabbits Are Comparable

et al. 1998

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The abilities of a parent and mutant pair of Staphylococcus epidermidis strains, the slime-producing parent RP62A and its slime-negative mutant, to establish endocarditis in a rabbit model of aortic valve endocarditis and to accumulate and adhere to surfaces in vitro were compared. Vegetation titer and infection rate depended on the presence or absence of a catheter (P = 0.020) and on inoculum size (P < 0.001) but not on the infecting strain. The ability of the parent strain vis-à-vis its mutant to accumulate in vitro on surfaces as demonstrated in a slime test did not correlate with any enhancement in the development of endocarditis in the rabbit model. In vitro initial adherence rates were identical. Both isolates accumulated to the same reduced extent in vitro in the presence of serum, albumin, or gelatin. Adhesion was equally promoted by addition of fibronectin. These data suggest that the in vitro phenomenon of accumulation described as slime production in the absence of serum may not be an important virulence determinant in vivo.

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“…S. aureus survival within the hospital environment is well documented [11][12][13], and some epidemic strains can remain viable for longer on cotton lint than other strains [14]. The mechanisms of enhanced survival are poorly understood, but resistance to desiccation [13,15], adherence [16], and the ability to survive on human skin [15][16][17] have been implicated.…”

Section: Neonatal Units Provide Ideal Environments Formentioning

confidence: 99%

A five year outbreak of methicillin-susceptible Staphylococcus aureus phage type 53,85 in a regional neonatal unit

et al. 2000

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Article ReuseUnless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. SUMMARYWe identified a 5-year outbreak of a methicillin-susceptible Staphylococcus aureus (MSSA) strain, affecting 202 babies on a neonatal unit, by routine weekly phage typing all S. aureus isolates. Multiple staged control measures including strict emphasis on hand hygiene, environmental and staff surveillance sampling, and application of topical hexachlorophane powder failed to end the outbreak. S. aureus PT 53,85 (SA5385) was found on opened packs of Stomahesive2, used as a neonatal skin protectant. Only following the implementation of aseptic handling of Stomahesive, and the use of topical mupirocin for staff nasal carriers of SA5385, and for babies colonized or infected with S. aureus, did the isolation rate of SA5385 decline. DNA fingerprinting indicated that 95 % of SA5385 isolates were clonal. In itro death rates of SA5385 on Stomahesive with human serum were significantly lower than on Stomahesive alone (P l 0n04), and on cotton sheet with serum (P l 0n04), highlighting the potential of this material as a survival niche. Phage typing remains a valuable, inexpensive and simple method for monitoring nosocomial MSSA infection.

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Lack of evidence for increased adherent growth in broth or human serum of clinically significant coagulase-negative staphylococci

Cited by 12 publications

References 41 publications

Impact of Bacterial Biofilm Formation on In Vitro and In Vivo Activities of Antibiotics

Impact of Bacterial Biofilm Formation on In Vitro and In Vivo Activities of Antibiotics

The Abilities of aStaphylococcus epidermidisWild-Type Strain and Its Slime-Negative Mutant To Induce Endocarditis in Rabbits Are Comparable

A five year outbreak of methicillin-susceptible Staphylococcus aureus phage type 53,85 in a regional neonatal unit

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