Lack of evidence for glial cells originating from the external granular layer in mouse cerebellum

Swarz, Jeffrey R.; Cerro, Manuel del

doi:10.1007/bf01261508

Cited by 34 publications

(14 citation statements)

References 28 publications

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“…We have also concluded from previous stud ies with an oligodendrocyte marker [14][15][16][17] that cerebellar oligodendrocytes do not orig inate from EGL stem cells. Our results would confirm the hypothesis of Ramon y Cajal [30] and the observations of Del Cerro and Snider [10] and Swarz and Del Cerro [33] suggesting that glial cells do not derive from the EGL. Autoradiography experiments with labelled thymidine for assessing the exact birth date of astrocytes are in progress.…”

Section: Discussionsupporting

confidence: 82%

A Biochemical and Immunohistological Study of S100 Protein in Developing Rat Cerebellum

et al. 1981

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Specific immune sera, one directed against S100 protein and one against glial fibrillary acidic protein (both proteins are specific astrocytic markers in rat cerebellum), were used in immunohistological methods to follow the formation and maturation of astrocytes during the postnatal development of rat cerebellum. Throughout development, S100 protein is detected only in astrocytes. Immunofluorescent Golgi epithelial cells and other astrocytes are detected in the cerebellum of newborn rat, their number increases rapidly between birth and the 10th postnatal day and then more slowly until the end of the 3rd week. In contrast, the increase in S100 protein level, measured in cerebellar homogenates by radioimmunoassay, was small until the 10th postnatal day. The greater accumulation subsequently found corresponds to an increase in the content of each astrocyte during the process of maturation.

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Section: Discussionsupporting

confidence: 82%

A Biochemical and Immunohistological Study of S100 Protein in Developing Rat Cerebellum

et al. 1981

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“…Scale bar 5 653 µm for A-E, 595 µm for F, G. ule cells derived from postnatal mice (Gao et al, 1991). In the present study, EGF-R mRNA was detected within the cerebellar external granule cell layer (EGL), which has been reported to generate only neurons (Swarz and Del, 1977), making a role in neurogenesis likely. Hybridization of EGF-R cRNA in the EGL was present as early as the EGL itself was detectable, and our prior study indicated that this hybridization persists throughout the postnatal period of granule cell proliferation .…”

Section: Potential Functions Of Egf-rmentioning

confidence: 87%

“…The mRNA for EGF-R has also been localized within some of these areas (Kaser et al, 1992;Seroogy et al, 1994), but is additionally present within the proliferative germinal zones (gz) of the postnatal brain . These germinal zones include the subventricular zone (svz) of the neostriatum, which primarily produces glia during postnatal periods, as well as the external granule cell layer of the cerebellum (EGL), which produces neurons exclusively (Swarz and Del, 1977).…”

mentioning

confidence: 99%

Prenatal ontogeny of the epidermal growth factor receptor and its ligand, transforming growth factor alpha, in the rat brain

et al. 1997

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Transforming growth factor alpha (TGF alpha) interacts with the epidermal growth factor receptor (EGF-R) to produce its biological effects. TGF alpha induces the proliferation and differentiation of central nervous system (CNS) astrocytes and pluripotent stem cells, as well as the survival and differentiation of postmitotic CNS neurons. Both TGF alpha and EGF-R have been localized to the postnatal CNS. As the majority of CNS neuronal proliferation and migration occurs antenatally, we have examined the ontogeny of TGF alpha and EGF-R in the embryonic rat brain by in situ hybridization. EGF-R mRNA was expressed in the brain as early as embryonic day 11 (E11; the earliest age examined). It was initially detected in the midbrain, with subsequent expression first in multiple germinal zones, followed by expression in numerous cells throughout the brain. In many brain areas, EGF-R mRNA appeared in germinal centers during the later stages of neurogenesis and the early stages of gliogenesis. In the midbrain, the distribution of EGF-R mRNA overlapped extensively with that of tyrosine hydroxylase mRNA, suggesting that fetal dopaminergic neurons express EGF-R. Immunocytochemistry was used to demonstrate the presence of EGF-R-immunoreactive protein in brain areas that expressed EGF-R mRNA on E15 and E20. The expression of TGF alpha in many brain structures preceded that of EGF-R mRNA. TGF alpha mRNA was distributed throughout many non-germinal centers of the brain on E12 and later. Some brain areas, such as the external granule cell layer of the cerebellum, expressed EGF-R, but not TGF alpha mRNA. Northern blot analysis demonstrated that mRNA species for both TGF alpha and EGF-R were similar in embryos and adults. These data indicate that TGF alpha and EGF-R are positioned to have a role in the genesis, differentiation, migration, or survival of numerous cell populations in the embryonic brain.

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“…The area of the molecular layer was measured as described above and thus the total number of cell profiles in the nodulus and culmen of a vermis section was calculated. Since (a) very few glia or cell types other than basket and stellate cells are normally seen in the molecular layer [19,26], (b) gliosis was not prominent in 25-day-old MAM-treated animals, and (c) most granule cells migrated to the internal granular layer following MAM treatment, this measurement provides an estimate of the number of basket and stellate cell profiles. The estimate is more likely to be slightly high for MAMtreated animals than for control animals due to the greater probability of other cell types in the molecular layer.…”

Section: Morphological Studiesmentioning

confidence: 99%

Regeneration in the Cerebellum Following Methylazoxymethanol-Induced Destruction of the External Germinal Layer

1980

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In the cerebellum external germinal layer (EGL) regeneration was maximal 10 days following methylazoxymethanol-induced destruction of the postnatal mouse EGL. Similar patterns of EGL repair and delayed disappearance were observed in both anterior and posterior cerebellar lobes. Final reduction in granule cell number to 30–50% of control exceeded the reduction in basket and stellate cells. Morphological changes in GABAergic cell populations were paralleled by alterations in glutamic acid decarboxylase activity. These studies suggest that the regenerating EGL may be similar to the normal EGL with respect to the emerging cell types, but that granule cells which develop later are less completely represented.

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Lack of evidence for glial cells originating from the external granular layer in mouse cerebellum

Cited by 34 publications

References 28 publications

A Biochemical and Immunohistological Study of S100 Protein in Developing Rat Cerebellum

A Biochemical and Immunohistological Study of S100 Protein in Developing Rat Cerebellum

Prenatal ontogeny of the epidermal growth factor receptor and its ligand, transforming growth factor alpha, in the rat brain

Regeneration in the Cerebellum Following Methylazoxymethanol-Induced Destruction of the External Germinal Layer

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