Lack of Effect of Prior Treatment with Fluoride on Genotoxicity of Two Chemical Agents in vitro

Ribeiro, Daniel Araki; Marques, Sílvio Alencar; Salvadori, Daisy Maria Fávero

doi:10.1159/000099326

Cited by 6 publications

(3 citation statements)

References 38 publications

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“…Cepacol® and Plax® contain high concentration of sodium fluoride. Previous studies conducted by our group have demonstrated that sodium fluoride did not cause genetic damage either in vitro or in vivo [29,30]. Regardless of the biological phenomenon involved in this process, we assumed that Periogard® is a mutagenic agent as a result of chromosomal breakage or loss in buccal mucosa cells.…”

Section: Discussionmentioning

confidence: 91%

Cytogenetic damage induced by mouthrinses formulations in vivo and in vitro

et al. 2011

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The aim of the present study was to comparatively evaluate DNA damage and cellular death in cells exposed to various commercially available mouthrinses: Listerine Cepacol, Plax alcohol free, Periogard, and Plax Whitening. A total of 75 volunteers were included in the search distributed into five groups containing 15 people each for in vivo study. Exfoliated buccal mucosa cells were collected immediately before mouthrinse exposure and after 2 weeks. Furthermore, blood samples were obtained from three healthy donors for in vitro study. The micronucleus test was used to evaluate mutagenicity and cytotoxicity in vivo. The single-cell gel (comet) assay was used to determine DNA damage in vitro. After 2 weeks exposure, Periogard showed 1.8% of micronucleated cells with significant statistical differences (p < 0.05) compared to before exposure (0.27%). Plax Whitening presented high tail moment value (4.5) when compared to negative control (0.6). The addition of all mouthrinses to cells incubated with methyl methanesulfonate did not alter the number of strand breaks in the genetic material. Listerine was able to reduce genetic damage induced by hydrogen peroxide because a decrease of tail moment was noticed. The results of the present study suggest that Periogard and Plax Whitening can induce genetic damage, whereas Listerine is an antioxidant agent. Since DNA damage is considered to be prime mechanism during chemical carcinogenesis, these data may be relevant in risk assessment for protecting human health and preventing carcinogenesis.

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Section: Discussionmentioning

confidence: 91%

Cytogenetic damage induced by mouthrinses formulations in vivo and in vitro

et al. 2011

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“…Earlier reports regarding the genotoxic potential of F during in vitro (Anuradha et al, 2001;Ha et al, 2004;Liu et al, 2007;Machalinska et al, 2001) and in vivo (He and Chen, 2006) studies in humans (Gadhia and Joseph, 1997) and rodents (Podder et al, 2008(Podder et al, , 2011 are conflicting. Some studies demonstrated that F did not produce damage to the genetic material (Li et al, 1987;Ribeiro et al, 2006;Ribeiro et al, 2007;Skare et al, 1986). Other studies on oxidative stress in fluorotic humans and F-intoxicated animals indicated that generation of reactive oxygen species (ROS) and lipid peroxidation may be directly induced by F (Ailani et al, 2009;Inkielewicz et al, 2006;Podder et al, 2008;Susheela and Bhatnagar 2002).…”

Section: Please Scroll Down For Articlementioning

confidence: 99%

Sodium Fluoride Promotes Apoptosis by Generation of Reactive Oxygen Species in Human Lymphocytes

Jothiramajayam¹,

Sinha²,

Ghosh³

et al. 2014

Journal of Toxicology and Environmental Health, Part A

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Fluoride generated the attention of toxicologists due to its deleterious effects at high concentrations in human populations suffering from fluorosis and with in vivo experimental models. Interest in its undesirable effects has resurfaced due to the awareness that this element interacts with cellular systems even at low doses. This study focused on examining the adverse effects of inorganic fluoride (NaF) on human lymphocyte cells in vitro. Mitochondrial function, oxidative stress, cell cycle progression, and mode of cell death were combined with genotoxic endpoints. Data demonstrated that NaF at lower concentrations, although not significantly cytotoxic and genotoxic, induced oxidative stress leading to apoptotic cell death. The results also suggested that at low concentrations (<1 μg/ml), NaF may affect cell cycle progression. Taken together, our findings confirm earlier reports on mechanisms involved in NaF-induced apoptosis.

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“…However, it is well known that X-rays can induce cytotoxic effects and cause DNA damage. 2,3 Considering the strong evidence for a relationship between genetic damage/cytotoxicity and carcinogenesis, 4 an investigation of the noxious effects induced by dental X-rays is necessary to identify the degree of risk involved and minimize potential risks to patients and clinicians. The purpose of this article is to provide a review of the biomonitoring data of dental X-ray in oral mucosa cells, based on our recent research within the field.…”

Section: Introductionmentioning

confidence: 99%

Cytogenetic biomonitoring in oral mucosa cells following dental X-ray

Ribeiro¹

2012

Dentomaxillofacial Radiology

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Objectives: In the past decades, X-rays have been used widely for diagnosis in dentistry. However, it is well known that ionizing radiation causes damage (including single-and double-strand breaks) to deoxyribonucleic acid (DNA) and DNA-protein crosslinks, and induces cellular death. Therefore, outlining the cytogenetic effects induced by X-ray is necessary to identify the degree of cancer risk and minimize potential risks to patients and clinicians. To date, a variety of assays have been proposed in cytogenetic biomonitoring studies, including those that assess metaphase chromosomal aberrations and sister chromatid exchanges, and micronucleus and single-cell gel (comet) assay. Methods: Cytogenetic biomonitoring studies focusing on oral mucosa cells in individuals exposed to dental X-ray were reviewed. Results: Dental X-ray can induce DNA damage and cytotoxicity in oral mucosa cells. Conclusion: These results will contribute to a better understanding of X-ray-induced effects upon the cellular system in individuals continually exposed to known genotoxic/cytotoxic agents.

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Lack of Effect of Prior Treatment with Fluoride on Genotoxicity of Two Chemical Agents in vitro

Cited by 6 publications

References 38 publications

Cytogenetic damage induced by mouthrinses formulations in vivo and in vitro

Cytogenetic damage induced by mouthrinses formulations in vivo and in vitro

Sodium Fluoride Promotes Apoptosis by Generation of Reactive Oxygen Species in Human Lymphocytes

Cytogenetic biomonitoring in oral mucosa cells following dental X-ray

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