Lack of effect of  -chlorohydrin on the ATP content of rat, mouse and human spermatozoa

Chulavatnatol, Montri; Hasibuan, Ira Astuti; Yindepit, S.; Eksittikul, Thidarat

doi:10.1530/jrf.0.0500137

Cited by 23 publications

(8 citation statements)

References 12 publications

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“…However, for the initiation of motility, immature sperm also seem to require an endogenous trypsin-like activity similar to that of mature sperm, as suggested by the similar acceleration of motility initiation by trypsin; although such endogenous activities of immature and mature sperm have different sensitivities to ATP concentrations. I n view of the lack of a general trend in the changes in intracellular ATP content on maturation of sperm in the epididymis, for instance a decrease in guinea-pig sperm (Frenkel et al 1973), an increase in bovine sperm (Hoskins et al 1975) and an increase (Chulavatnatol et al 1977) or no change (Ke & Tso 1982) in rat sperm, the physiological significance of the present finding still has to be clarified. Nevertheless, the inhibitory effects of supraoptimal concentrations of ATP on axonemal function may explain the controversy concerning the correlation between ATP content and motility (see Introduction), although the mechanism of this action requires further elucidation.…”

Section: Discussionmentioning

confidence: 68%

Temporary inhibition of the initiation of motility of demembranated hamster sperm by high concentrations of ATP

Yeung

1986

Int J of Andrology

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ATP is obligatory for sperm movement. When sperm from the hamster cauda epididymis were washed and exposed to a demembranation and re-activation medium containing 0.5 or 1 mM ATP, the maximal % motility was attained almost immediately. Increasing the ATP concentration to 3 mM caused a 5 min delay in development of maximal re-activation and a change in the beating pattern as indicated by a decrease in the % of forward progressing sperm, decreased linear velocities but unchanged curvilinear velocities. The inhibition of motility initiation by 3 mM ATP was abolished either by the inclusion of trypsin (50 ng/ml) or by a brief pre-incubation of the sperm in the demembranation and reactivation medium before the addition of ATP. However, the beneficial effect of pre-incubation was prevented if trypsin inhibitor was present. This indicates that the inhibition by ATP acts via a trypsin-sensitive regulatory system, and suggests that an endogenous protease activity is necessary to overcome this. Re-activation of sperm from the caput epididymis by all three concentrations of ATP showed similar lag periods which could also be diminished by pre-incubation or trypsin. The results suggest that, in addition to developing their motile capacity during maturation in the epididymis, sperm exonemes change their sensitivity to ATP concentrations.

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Section: Discussionmentioning

confidence: 68%

Temporary inhibition of the initiation of motility of demembranated hamster sperm by high concentrations of ATP

Yeung

1986

Int J of Andrology

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“…Changes in hexokinase isozymt. In rat (Chulavatnatol et al 1977) and bull (Hoskins et al 1975), the level of ATP is higher in caudal than in caput sperm. Studies of pyruvate kinase isozymes in developing chick skeletal muscle have also indicated isozymic shifts both in vivo and in culture (Cardenas et al 1979).…”

Section: Discussionmentioning

confidence: 90%

“…Very little is known concerning the activation of enzyme during epididymal passage. In rat (Chulavatnatol et al 1977) and bull (Hoskins et al 1975), the level of ATP is higher in caudal than in caput sperm. It has been suggested that in bovine caput sperm, hexokinase is inhibited due to the limited amount of ATP available as a cosubstrate (Hoskins et al 1975).…”

Section: Discussionmentioning

confidence: 90%

Increase in Sperm Type Hexokinase Activity of Rat Spermatozoa During Maturation

Mongkolsirikieat¹,

Boonsaeng²

1980

Int J of Andrology

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Rat hexokinases fro caput sperm (immature) and caudal sperm (mature) were investigated. The hexokinases from both sources were studied by DEAE-cellulose column chromatography and by cellulose acetate electrophoresis. The specific activity of caput sperm hexokinase was not significantly different from that of the caudal sperm enzyme. Spermatozoa possess two isozymes of hexokinase. Type I hexokinase was the predominant type in caput sperm whereas the sperm type of hexokinase was predominant in caudal sperm. Hexokinase types II and III were absent in both extracts.

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“…Spermatozoa were extruded from adult albino rats into phosphate-buffered saline (PBS) containing 015 M-NaCl in 5 mM-phosphate, pH 7-4 at 4°C as previously described (Chulavatnatol, Hasibuan, Yindepit & Eksittikul, 1977 (Table 1). During sperm maturation, the calculated number of anionic sites per cell increased by 20% but there was a decrease in the binding constant estimated according to the method of Grinnell & Hays (1979).…”

Section: Methodsmentioning

confidence: 99%

Measurement of anionic sites of rat epididymal spermatozoa using tritiated polycationized ferritin

Toowicharanont¹,

Chulavatnatol²

1983

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Lack of effect of -chlorohydrin on the ATP content of rat, mouse and human spermatozoa

Cited by 23 publications

References 12 publications

Temporary inhibition of the initiation of motility of demembranated hamster sperm by high concentrations of ATP

Temporary inhibition of the initiation of motility of demembranated hamster sperm by high concentrations of ATP

Increase in Sperm Type Hexokinase Activity of Rat Spermatozoa During Maturation

Measurement of anionic sites of rat epididymal spermatozoa using tritiated polycationized ferritin

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