Lack of Cross-Species Transmission of Porcine Endogenous Retrovirus Infection to Nonhuman Primate Recipients of Porcine Cells, Tissues, or Organs1

Switzer, William M.; Michler, Robert E.; Shanmugam, Vedapuri; Matthews, Aprille; Hussain, Althaf I.; Wright, Anthony C.; Sandstrom, Paul; Chapman, Louisa E.; Weber, Collin J.; Safley, Susan A.; Denny, Roger R.; Navarro, Albert Bosch i; Evans, Valerie; Norin, Allen J.; Kwiatkowski, Paweł; Heneine, Walid

doi:10.1097/00007890-200104150-00022

Cited by 97 publications

(61 citation statements)

References 35 publications

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“…These studies stand in contrast to the lack of infection detected in nonhuman primates (12)(13)(14)(15) and several small laboratory animal species (12,16). Similarly, it has not been possible to demonstrate PERV infection in patients exposed to living porcine tissues (17)(18)(19)(20)(21)(22)(23).…”

Section: Introductionmentioning

confidence: 84%

Mouse retrovirus mediates porcine endogenous retrovirus transmission into human cells in long-term human-porcine chimeric mice

Yang

Wood²,

Lan³

et al. 2004

J. Clin. Invest.

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Porcine endogenous retrovirus (PERV) is a potential pathogen in clinical xenotransplantation; transmission of PERV in vivo has been suggested in murine xenotransplantation models. We analyzed the transmission of PERV to human cells in vivo using a model in which immunodeficient NOD/SCID transgenic mice were transplanted with porcine and human lymphohematopoietic tissues. Our results demonstrate, we believe for the first time, that human and pig cells can coexist long-term (up to 25 weeks) without direct PERV infection of human cells. Despite the transplantation of porcine cells that did not produce human-tropic PERV, human cells from the chimeric mice were frequently found to contain PERV sequences. However, this transmission was due to the pseudotyping of PERV-C (a virus without human tropism) by xenotropic murine leukemia virus, rather than to de novo generation of human-tropic PERV. Thus, pseudotyping might account for the PERV transmission previously observed in mice. The absence of direct human cell infection following long-term in vivo coexistence with large numbers of porcine cells provides encouragement regarding the potential safety of using pigs that do not produce human-tropic PERV as source animals for transplantation to humans.

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Section: Introductionmentioning

confidence: 84%

Mouse retrovirus mediates porcine endogenous retrovirus transmission into human cells in long-term human-porcine chimeric mice

Yang

Wood²,

Lan³

et al. 2004

J. Clin. Invest.

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“…islet cells, insulin, skin, extracorporal liver) for up to 12 years [42]. Recent intensive research has shown that porcine endogenous retroviruses probably do not present a risk for recipients of xenotransplants provided all necessary precautions are taken [43][44][45][46]. In addition, a strain of miniature pigs has been identified that does not produce infective PERV [47].…”

Section: Production Of a New Class Of Antibiotics: Cationic Antimicromentioning

confidence: 99%

The contribution of farm animals to human health

Kues¹

2004

Trends in Biotechnology

117

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“…However, co-culture of human cell line and cells from SNU miniature pigs showed that the virus was non-replicable and non-productive in human cells (7). Also, PERV has not been reported the infection to human and non-human primates in vivo (6,8,9).…”

Section: Introductionmentioning

confidence: 99%

Analysis ofenvSubtypes of Porcine Endogenous Retrovirus in SNU Miniature Pigs

Park

Hwang

2014

J Bacteriol Virol

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All xenografts from pigs impose infection risk by porcine endogenous retrovirus (PERV). The purpose of this study was to investigate the env constructs with the comparison of the ratio of the competent form to the defective one of env in subtypes, PERV-A, PERV-B and PERV-C in different pig breeds. The results of PCR amplification of env represented that all env subtypes had more than two defective forms which cannot bind to host cells due to the absence of binding regions of env in miniature pigs, SNU and PWG, and farm pig breeds, Duroc, Yorkshire and Landrace. In addition, comparing the full sequences with the defective ones in three subtypes demonstrated that the present percentages of env sequences in defective PERV-A, PERV-B and PERV-C were approximately 50%, 38~45% and 4~11%, respectively, in SNU and PWG pigs whereas PERV-A and PERV-B occupied around 40 to 60% but PERV-C was not detected in farm pigs. Quantitative real-time PCR assays with primers and probes targeted to proline-rich region (PRR) of each env subtype were done to measure the copy numbers of each env subtype. When the reference was set with copy number of PERV-A, the ratio of those of PERV-B and PERV-C to the reference were 1.5 to 6.0 folds high in SNU and PWG pigs while 1.0 or less in farm pigs. These contradictory results of PERV-C constructs and copy numbers in SNU pigs suggests that many truncated or short defective sequences of PERV-C might be present in them.

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Lack of Cross-Species Transmission of Porcine Endogenous Retrovirus Infection to Nonhuman Primate Recipients of Porcine Cells, Tissues, or Organs1

Cited by 97 publications

References 35 publications

Mouse retrovirus mediates porcine endogenous retrovirus transmission into human cells in long-term human-porcine chimeric mice

Mouse retrovirus mediates porcine endogenous retrovirus transmission into human cells in long-term human-porcine chimeric mice

The contribution of farm animals to human health

Analysis ofenvSubtypes of Porcine Endogenous Retrovirus in SNU Miniature Pigs

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