Lack of correlation between plasmid-encoded outer membrane proteins and virulence of Yersinia enterocolitica

Mazigh, D; Chalvignac, M A; Quilici, M; Mollaret, H.H.

doi:10.1016/s0769-2609(85)80004-1

Cited by 2 publications

(6 citation statements)

References 14 publications

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“…3, lane 5). pseudotuberculosis strains all exhibited three major proteins (25,37, and 47 kDa for Y. enterocolitica and 26, 34, and 47 kDa for Y. pseudotuberculosis) and three to four minor proteins (30,34,38,57, and 67 kDa).…”

Section: Resultsmentioning

confidence: 97%

“…The labeled minicell products were immunoprecipitated with anti-0:9RP and analyzed by SDS-PAGE. Three major proteins (25,37, and 47 kDa for Y. enterocolitica and 26, 34, and 47 kDa for Y. pseudotuberculosis) were precipitated by antibodies. These protein bands are virtually identical in molecular mass to those released by Y. enterocolitica and Y. pseudotuberculosis.…”

Section: Resultsmentioning

confidence: 99%

“…By using the E. coli minicell system in combination with precipitating antibodies to Yersinia RPs, conclusive evidence was obtained that at least the three major proteins of the released fraction of Y. enterocolitica (25,37, and 47 kDa) and Y. pseudotuberculosis (26,34, and 47 kDa) are plasmid encoded. Of course, it is conceivable that there are more than three released major RPs that are plasmid encoded, but they could not be detected because of inefficient expression in E. coli.…”

Section: Discussionmentioning

confidence: 99%

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Immunochemical analysis of plasmid-encoded proteins released by enteropathogenic Yersinia sp. grown in calcium-deficient media

Heesemann¹,

Groß²,

Schmidt³

et al. 1986

Infect Immun

157

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Enteropathogenic Yersinia sp. releases plasmid-associated proteins of low molecular mass (26-67 kilodaltons) at 37°C. In this study, the optimum conditions for the release of proteins were assessed and the released proteins (RPs) were analyzed for the manner of release, immunochemical characteristics, and the location of the genes necessary for their synthesis. Protein release was strongly enhanced when growth media were markedly depleted of calcium ions by precipitation with oxalate or chelation with EGTA [ethylene glycol-bis(paminoethyl ether)-N,N,N',N'-tetraacetic acid]. RP yields were greatest when Yersinia spp. were in the exponential growth phase. The RPs appeared to be released from the Yersinia spp. by secretion rather than by pinching off of membrane vesicles, because the RPs did not sediment during high-speed centrifugation nor were they contaminated to any signfficant degree with lipopolysaccharide. Moreover, immunoblot analysis revealed only traces of protein species related to RPs within the outer membranes of plasmid-positive Yersinia spp. grown at 37°C under calcium-restricted conditions. Immunoblot studies also showed that the RPs of Y. enterocolitica serotypes 0:3, 0:8, and 0:9 and the RP of Y. pseudotuberculosis serotype I are highly cross-reactive. Finally, the immunoprecipitates of the products of minicells which harbor Yersinia plasmids were used to demonstrate that at least three proteins immunochemically related to the released fraction were plasmid encoded. These results suggest that at least three of the RPs may be related to or identical with previously described plasmid-encoded Yersinia outer membrane proteins.MATERIALS AND METHODS Bacterial strains and culture conditions. Y. enterocolitica serotypes 0:3 (Y-108 Nalr, plasmid positive), 0:9 (Y-96 Nalr, plasmid positive), and 0:8 (WA-314 Nalr, plasmid positive, and its plasmid-negative derivative WA-cNalr) 561 on August 5, 2020 by guest

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Immunochemical analysis of plasmid-encoded proteins released by enteropathogenic Yersinia sp. grown in calcium-deficient media

Heesemann¹,

Groß²,

Schmidt³

et al. 1986

Infect Immun

157

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“…Several studies have shown an unclear correlation between established virulence markers and diarrheal illness in Y. enterocolitica (Bissett er al. 1990;Kwaga and Iversen 1991;Mazigh et al 1985;Morris et al 1991;Noble et al 1987;Prpic et al 1985). Here the relationship between the presence of virulence plasmid in Y. enterocolitica, its phenotypic properties and pathogenicity was investigated using Tn801-inserted derivatives of the virulence plasmid.…”

Section: Resultsmentioning

confidence: 99%

“…These characteristics have been used to distinguish between plasmid-bearing virulent and plasmidless avirulent strains and also have been correlated with the pathogenicity of the organism viz the mouse virulence test (Bhaduri 1990a;Bhaduri et al 1987;Doyle and Cliver 1990;Kapperud 1991;Kwaga and Iversen 1991;Lazere and Gemski 1983;Mazigh et al 1983;Portnoy and Martinez 1985;Robins-Browne et al 1989); though the mechanisms involved invirulence remain unclear. Studies by several investigators (Bissett et al 1990;Kwaga and Iversen 1991;Mazigh et al 1985;Morris et al 1991;Noble et al 1987;Prpic et al 1985) suggest that the presence of the virulence plasmid and its expression in Y. enterocolitica may not unequivocally indicate pathogenicity. Hence, it is important to further evaluate these correlations for the elucidation of the pathogenicity of Y. enterocolitica.…”

Section: Introductionmentioning

confidence: 99%

LACK OF CORRELATION BETWEEN PLASMID‐ASSOCIATED PHENOTYPES OF YERSINIA ENTEROCOLITICA AND PATHOGENICITY IN THE MOUSE¹

Bhaduri

1996

Journal of Food Safety

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Various phenotypic characteristics have been correlated with the pathogenicity of plasmid‐bearing virulent strains of Yersinia enterocolitica. Two transposon Tn801‐insertion derivatives (JD193 and JD217) of the virulence plasmid from serotype 0:3 were used to determine the correlation between pathogenicity and plasmid‐associated properties of this organism. Both Tn801‐inserted derivatives expressed five plasmid‐associated phenotypic characteristics at 37C: (1) colony morphology, (2) calcium‐dependent growth or low‐calcium response, (3) crystal violet binding, (4) autoagglutination, and (5) hydrophobicity. However, for mouse pathogenicity only JD193 was positive whereas, JD217 was avirulent for mice. Thus, it is possible to have a lack of correlation between plasmid‐mediated traits and the actual pathogenicity of the organism in the mouse; however, these plasmid‐mediated phenotypic characteristics provide simple and efficient techniques to evaluate the virulence potential of wild‐type strains isolated from food poisoning outbreaks and clinical cases.

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Lack of correlation between plasmid-encoded outer membrane proteins and virulence of Yersinia enterocolitica

Cited by 2 publications

References 14 publications

Immunochemical analysis of plasmid-encoded proteins released by enteropathogenic Yersinia sp. grown in calcium-deficient media

Immunochemical analysis of plasmid-encoded proteins released by enteropathogenic Yersinia sp. grown in calcium-deficient media

LACK OF CORRELATION BETWEEN PLASMID‐ASSOCIATED PHENOTYPES OF YERSINIA ENTEROCOLITICA AND PATHOGENICITY IN THE MOUSE¹

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Lack of correlation between plasmid-encoded outer membrane proteins and virulence of Yersinia enterocolitica

Cited by 2 publications

References 14 publications

Immunochemical analysis of plasmid-encoded proteins released by enteropathogenic Yersinia sp. grown in calcium-deficient media

Immunochemical analysis of plasmid-encoded proteins released by enteropathogenic Yersinia sp. grown in calcium-deficient media

LACK OF CORRELATION BETWEEN PLASMID‐ASSOCIATED PHENOTYPES OF YERSINIA ENTEROCOLITICA AND PATHOGENICITY IN THE MOUSE1

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LACK OF CORRELATION BETWEEN PLASMID‐ASSOCIATED PHENOTYPES OF YERSINIA ENTEROCOLITICA AND PATHOGENICITY IN THE MOUSE¹