Lack of CaBP1/Caldendrin or CaBP2 Leads to Altered Ganglion Cell Responses

Sinha, Raunak; Lee, Amy; Haeseleer, Françoise

doi:10.1523/eneuro.0099-16.2016

Cited by 15 publications

(16 citation statements)

References 60 publications

(107 reference statements)

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“…The procedures used in this study are not expected to produce pain or suffering in the animals. Generation of CaBP1 KO mice (RRID: MGI: 5780462) and CaBP2 KO mice was described previously (Kim et al, 2014;Sinha et al, 2016). Mice were maintained on a C57BL/6N (for CaBP1 KO) or C57BL/6J (for CaBP2 KO) background strains and housed in groups on a standard 12:12 hour light: dark cycle, with food and water provided ad libitum.…”

Section: Animalsmentioning

confidence: 99%

“…We next analyzed ABRs and DPOAEs of CaBP2 KO mice. These mice were generated by replacing exons 1 and 2A with tdTomato and PGK neo cassette as well as stop codons in all 3 reading frames of exon 2B, which should prevent expression of all CaBP2 splice variants (Sinha et al, 2016). In contrast to the normal ABRs of 4 week-old CaBP1 KO mice ( Fig.…”

Section: Abrs Indicate Progressive Hearing Loss In Cabp2 Ko Micementioning

confidence: 99%

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Functions of CaBP1 and CaBP2 in the peripheral auditory system

Yang

Pangršič

et al. 2018

Hearing Research

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CaBPs are a family of Ca binding proteins related to calmodulin. Two CaBP family members, CaBP1 and CaBP2, are highly expressed in the cochlea. Here, we investigated the significance of CaBP1 and CaBP2 for hearing in mice lacking expression of these proteins (CaBP1 KO and CaBP2 KO) using auditory brain responses (ABRs) and distortion product otoacoustic emissions (DPOAEs). In CaBP1 KO mice, ABR wave I was larger in amplitude, and shorter in latency and faster in decay, suggestive of enhanced synchrony of auditory nerve fibers. This interpretation was supported by the greater excitability of CaBP1 KO than WT neurons in whole-cell patch clamp recordings of spiral ganglion neurons in culture, and normal presynaptic function of CaBP1 KO IHCs. DPOAEs and ABR thresholds were normal in 4-week old CaBP1 KO mice, but elevated ABR thresholds became evident at 32 kHz at 9 weeks, and at 8 and 16 kHz by 6 months of age. In contrast, CaBP2 KO mice exhibited significant ABR threshold elevations at 4 weeks of age that became more severe in the mid-frequency range by 9 weeks. Though normal at 4 weeks, DPOAEs in CaBP2 KO mice were significantly reduced in the mid-frequency range by 9 weeks. Our results reveal requirements for CaBP1 and CaBP2 in the peripheral auditory system and highlight the diverse modes by which CaBPs influence sensory processing.

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Section: Animalsmentioning

confidence: 99%

Section: Abrs Indicate Progressive Hearing Loss In Cabp2 Ko Micementioning

confidence: 99%

Functions of CaBP1 and CaBP2 in the peripheral auditory system

Yang

Pangršič

et al. 2018

Hearing Research

Self Cite

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show abstract

“…CaBPs are highly expressed in the retina where they may regulate the contribution of Ca v 1 channels to neurotransmitter release at ribbon synapses (Haeseleer et al, 2004; Haeseleer et al, 2000; Rieke et al, 2008; Sinha et al, 2016). CaBPs are also expressed in cochlear hair cells, where it was hypothesized that their antagonism of CaM may account for the very limited CDI of Ca v 1.3 channels (Cui et al, 2007; Yang et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

CaBP1 regulates Cav1 L-type Ca2+ channels and their coupling to neurite growth and gene transcription in mouse spiral ganglion neurons

Yang

Choi

Soh

et al. 2018

Molecular and Cellular Neuroscience

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“…The success of retinal implant (a method of artificially replacing the function of retinal neurons, lost due to degenerative disease) depends on how effectively the prosthetic device maps the electrical stimulation pattern that can closely match the activity of RGC in terms of visual sensation. The recorded observations and temporal response of bipolar cells of RGCs revealed that the irradiance of the visual stimulus is proportional to the integrated voltage of PD of CIS [34] and the response of cells is non‐linear [35].…”

Section: Application Of the Proposed Mathematical Model As Activationmentioning

confidence: 99%