Lack of Association of NOS3 and ACE Gene Polymorphisms with Coronary Artery Disease in Southern Tunisia

Abdelhédi, Rania; Kharrat, Najla; Bouayed, Nouha; Abid, Leïla; Abdelmouleh, Wafa; Sahnoun, I.; AlFadhli, Suad; Laroussi, Lobna; Rebai, Ahmed

doi:10.1007/s10528-012-9545-x

Cited by 7 publications

(4 citation statements)

References 40 publications

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“…Also, greater red blood cell aggregability potential has been reported in CAD patients carrying the T allele of eNOS G894T polymorphism [ 24 ]. However, our results were inconsistent with the findings from some other studies that have reported a negative association between this polymorphism and CAD [ 12 , 13 , 18 ]. The reasons for these contradictory results may be related to variation in study design, different selection criteria for cases and controls, heterogeneity in sample size, and numerous known and unknown gene-gene and gene-environmental interactions [ 25 ].…”

Section: Discussioncontrasting

confidence: 99%

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Association of Nitric Oxide Levels and Endothelial Nitric Oxide Synthase G894T Polymorphism with Coronary Artery Disease in the Iranian Population

Mahmoodi

Nasehi

Karami

et al. 2016

VSI

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Purpose:The endothelial nitric oxide synthase (eNOS) G894T polymorphism has been reported to cause endothelial dysfunction and may have a role in the development of coronary artery disease (CAD). The aim of the present study was to investigate the association of eNOS G894T genetic polymorphism and plasma levels of nitric oxide (NO) with CAD risk in an Iranian population.Materials and Methods:We studied 200 patients with angiographically documented CAD and 100 matched controls. Analysis of G894T genetic polymorphism of eNOS was performed by polymerase chain reaction-restriction fragment length polymorphism method. Plasma levels of NO were determined using Griess method. Biochemical analysis was conducted by routine colorimetric methods.Results:Plasma levels of NO were significantly lower in CAD patients than control subjects (41.60±12.70 vs. 55.48±16.57, P=0.001). Also, the mean plasma levels of NO were significantly lower in T allele carriers of eNOS G894T polymorphism than G allele carriers (P<0.001). The genotype distribution and minor T allele frequency of eNOS G894T polymorphism significantly differed between CAD patients and control subjects (P<0.05). However, no significant association was found between the eNOS G894T polymorphism and the severity of CAD (number of diseased vessel) or the lipid profile of CAD patients (P>0.05).Conclusion:Reduced plasma level of NO is associated with increased risk of CAD in our population. Moreover, eNOS G894T polymorphism is a significant risk factor for CAD development via reducing the plasma levels of NO. However, eNOS G894T polymorphism is not a contributing factor for the severity of CAD.

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Section: Discussioncontrasting

confidence: 99%

“…The association of eNOS G894T gene polymorphism with CAD risk has been investigated in different populations; however, variable results of association [ 7 – 11 ] and lack of association [ 12 , 13 , 18 ] have been reported. The results of the present study strongly suggest that eNOS G894T polymorphism is a risk factor for CAD.…”

Section: Discussionmentioning

confidence: 99%

Association of Nitric Oxide Levels and Endothelial Nitric Oxide Synthase G894T Polymorphism with Coronary Artery Disease in the Iranian Population

Mahmoodi

Nasehi

Karami

et al. 2016

VSI

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“…The results from recent Tunisian studies provided conflicting results and did not consider the relationship between the ACE gene and traditional risk factors of CAD. 12,13…”

Section: Introductionmentioning

confidence: 99%

The Effect of ACE I/D Polymorphisms Alone and With Concomitant Risk Factors on Coronary Artery Disease

Amara

Mrad

Sayeh

et al. 2016

Clin Appl Thromb Hemost

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“…[181920] The reasons behind these inconsistent results remain to be determined. Yet, numerous factors such as variation in study design, different selection criteria to define CAD patients and control subjects, differences in sample size, numerous gene-gene and gene-environmental interactions, and ethnic background of studied populations may explain the heterogeneity of association studies.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the role of plasma nitric oxide levels, T-786C genetic polymorphism, and gene expression levels of endothelial nitric oxide synthase in the development of coronary artery disease

2017

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Background:Reduced bioavailability of nitric oxide (NO) and the T-786C polymorphism of endothelial nitric oxide synthase (eNOS) gene have been reported as risk factors for the development of coronary artery disease (CAD) with conflicting results. We investigated the association of plasma NO levels, T-786C genetic polymorphism, and gene expression levels of eNOS with CAD risk in an Iranian subpopulation.Materials and Methods:Studied population included 100 patients with angiographically verified CAD and 100 ethnically matched controls. Analysis of T-786C genetic polymorphism and gene expression levels of eNOS was conducted by polymerase chain reaction (PCR) restriction fragment length polymorphism and real-time reverse transcription-PCR methods, respectively. Plasma levels of NO were measured using Griess method.Results:The CC genotype distribution (15% vs. 6%, P = 0.011) and minor C allele frequency (36.5% vs. 21.5%, P = 0.001) of eNOS T-786C polymorphism differed significantly between CAD patients and control. Furthermore, eNOS T-786C polymorphism was more common among smoker than nonsmoker CAD patients (27.7% vs. 7.8%, P = 0.044). The association of the eNOS T-786C polymorphism with the severity of CAD (number of diseased vessel) was significant (P < 0.05). The gene expression levels of eNOS were significantly lower in the heterozygote (0.49 ± 0.1, P = 0.023) and mutant homozygote (0.36 ± 0.09, P = 0.011) genotypes than that of wild-type genotype (P < 0.05). In addition, NO levels were significantly lower in CAD patients compared with control subjects (42.62 ± 12.26 vs. 55.48 ± 16.57, P = 0.002) and showed intergenotypic variation in the CAD patients.Conclusion:Our study indicated that reduced NO levels and eNOS T-786C genetic polymorphism are significant risk factors for the development and severity of CAD in the Iranian population.

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Lack of Association of NOS3 and ACE Gene Polymorphisms with Coronary Artery Disease in Southern Tunisia

Cited by 7 publications

References 40 publications

Association of Nitric Oxide Levels and Endothelial Nitric Oxide Synthase G894T Polymorphism with Coronary Artery Disease in the Iranian Population

Association of Nitric Oxide Levels and Endothelial Nitric Oxide Synthase G894T Polymorphism with Coronary Artery Disease in the Iranian Population

The Effect of ACE I/D Polymorphisms Alone and With Concomitant Risk Factors on Coronary Artery Disease

Assessment of the role of plasma nitric oxide levels, T-786C genetic polymorphism, and gene expression levels of endothelial nitric oxide synthase in the development of coronary artery disease

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