Lack of Association of Angiotensin Converting Enzyme Gene Polymorphism or Serum Enzyme Activity With Coronary Artery Disease in Japanese Subjects

Abstract

The association of an insertion/deletion (I/D) polymorphism in the angiotensin converting enzyme (ACE) gene or the serum activity of ACE with coronary artery disease (CAD) was investigated in Japanese men and women. The ACE genotype of 947 CAD subjects who underwent coronary angiography and of 893 control subjects was determined by polymerase chain reaction analysis. No association of the DD genotype or the D allele with CAD was observed in men or women. In a low risk group (defined by a body mass index below the median value and the absence of a history of hypertension, diabetes mellitus, and hypercholesterolemia), there was also no association between the ACE gene polymorphism and CAD. No significant difference in serum ACE activity was detected between CAD subjects and controls of all genotypes or of the same genotype, whereas a significant association was apparent between serum ACE activity and ACE genotype for both CAD subjects and controls among both men and women. These results indicate that the ACE I/D polymorphism and genotype associated variation in serum ACE activity are not risk factors for CAD in Japanese men or women.

A Modern Understanding of the Traditional and Nontraditional Biological Functions of Angiotensin-Converting Enzyme

“…In two large studies from Copenhagen, a case-referent study (n = 10,150) and a retrospective cohort study (n = 7263), there was no evidence of an association of the ACE I/D gene polymorphism and myocardial infarction or ischemic heart disease in either males or females (Agerholm-Larsen et al, 1997). In a Japanese study of coronary artery disease in subjects who underwent coronary angiography (n = 947), there was no association between ACE genotype or serum ACE activity and disease (Fujimura et al, 1997). Arnett et al (1998) found no evidence that the ACE I/D polymorphism was associated with carotid intima-media thickness in a sample of middle-aged adults with no history of coronary artery disease (n = 495).…”

Five polymorphisms in gene candidates for cardiovascular disease in Afro‐Brazilian individuals

“…The ACE Ins/Del polymorphism has been correlated with variations in ACE activity and blood pressure, and risks for myocardial infarction and other cardiovascular disorders (10,11,(38)(39)(40). However, other studies have failed to demonstrate such associations in different populations (14)(15)(16)41). These discrepant results may be related to the different ethnic origins of the studied groups.…”

Association between polymorphisms of the renin–angiotensin system and coronary artery disease in Chinese patients with type 2 diabetes

“…32 A previously meta-analysis of 15 studies (data from 3394 patients with myocardial infarction and 5479 32 in 1994. In contrast with all these results, Fujimura et al 34 concluded that the ACE I/D polymorphism is not an important determinant of CAD. Several reasons may account for the positive association in our study compared with some previous data concerning the negative association of ACE gene polymorphism with CAD.…”