LACK OF ASSOCIATION BETWEEN THE TLR4
            <sup>D/D</sup>
            GENOTYPE AND INCREASED SUSCEPTIBILITY TO ESCHERICHIA COLI BLADDER INFECTIONS IN (C3H/HEJ X BALB/CANN)F
            <sub>1</sub>
            X C3H/HEJ BACKCROSS MICE

Suhs, Kaleigh; Marthaler, Brodie; Hopkins, Walter J.

doi:10.1016/s0022-5347(09)60669-8

Cited by 1 publication

(1 citation statement)

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“…To reiterate what has been previously described, TLR-4 is the primary receptor for recognizing LPS and is used in a substantial number of MIA studies. The C3H/HeJ mouse strain (and associated strains) possess a point mutation in the Tlr4 gene that renders the TLR4 receptor unresponsive to LPS and consequently, these mice become more susceptible to gram-negative infections such as, Escherichia coli (Suhs et al, 2011). It has also been documented that C57BL/6 mice are inherently more susceptible to infection from Mycobacterium paratuberculosis as compared to C3H/HeN mice (Veazey et al, 1995).…”

Section: Parental Genetics and Impact On The Immune Systemmentioning

confidence: 99%

A comprehensive approach to modeling maternal immune activation in rodents

et al. 2022

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Prenatal brain development is a highly orchestrated process, making it a very vulnerable window to perturbations. Maternal stress and subsequent inflammation during pregnancy leads to a state referred to as, maternal immune activation (MIA). If persistent, MIA can pose as a significant risk factor for the manifestation of neurodevelopmental disorders (NDDs) such as autism spectrum disorder and schizophrenia. To further elucidate this association between MIA and NDD risk, rodent models have been used extensively across laboratories for many years. However, there are few uniform approaches for rodent MIA models which make not only comparisons between studies difficult, but some established approaches come with limitations that can affect experimental outcomes. Here, we provide researchers with a comprehensive review of common experimental variables and potential limitations that should be considered when designing an MIA study based in a rodent model. Experimental variables discussed include: innate immune stimulation using poly I:C and LPS, environmental gestational stress paradigms, rodent diet composition and sterilization, rodent strain, neonatal handling, and the inclusion of sex-specific MIA offspring analyses. We discuss how some aspects of these variables have potential to make a profound impact on MIA data interpretation and reproducibility.

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Section: Parental Genetics and Impact On The Immune Systemmentioning

confidence: 99%