Lack of Association between Prohibitin 3′ Untranslated Region C→T Polymorphism and Breast Cancer in a Turkish Population

Abstract

The 3' untranslated region (3'UTR) of the prohibitin gene has a positive effect on arresting cell proliferation between G1 and S phases and inhibits DNA synthesis. A C-to-T transition within this region creates a variant that alters mRNA function and has been shown to be associated with an increased breast cancer risk among young North Americans who are under 50 years and have at least one first-degree relative with breast cancer. We carried out a population-based case-control study to assess whether this association exists in Turkish women. We examined 106 breast cancer patients and 154 healthy controls by PCR and restriction fragment length polymorphism analysis. In the prohibitin 3'UTR, we did not detect a difference in CT/TT genotype frequency (p = 0.694; odds ratio [OR], 1.106; 95% confidence intervals [CI], 0.659-1.86) or in C/T allele frequency (p = 0.850; OR, 1.043; 95% CI, 0.667-1.62) between the all breast cancer patients and the controls. The results did not change in subgroups defined by age or family history. Hence our results do not lend support to the hypothesis that this polymorphism contributes to risk of breast cancer. The prohibitin T variant is not associated with the risk of breast cancer in Turkish women.

Relationship Between the Prohibitin 3' Untranslated Region C > T Gene Polymorphism and Cancer Susceptibility - Results of a Meta-analysis

“…However, Campbell et al (2003) didn't find the 3' untranslated region C > T gene polymorphism of PHB was associated with the risk of breast cancer. Karakus et al (2008) reported that 3' UTR C > T gene polymorphism contributed to risk of breast cancer, and the PHB T variant was not associated with the risk of breast cancer in Turkish women.…”

“…Six studies (Campbell et al, 2003;Spurdle et al, 2003;Jakubowska et al, 2007;Grimm et al, 2008;Jakubowska et al, 2008;Karakus et al, 2008) were recruited into our investigation to study the relationship between prohibitin 3' untranslated region C > T gene polymorphism and cancer susceptibility. Three studies (Campbell et al, 2003;Jakubowska et al, 2007;Karakus et al, 2008) were performed in breast cancer and three studies (Spurdle et al, 2003;Grimm et al, 2008;Jakubowska et al, 2008) were conducted in ovarian cancer.…”

“…Three studies (Campbell et al, 2003;Jakubowska et al, 2007;Karakus et al, 2008) were performed in breast cancer and three studies (Spurdle et al, 2003;Grimm et al, 2008;Jakubowska et al, 2008) were conducted in ovarian cancer. The data of our interest were extracted: first author's surname, year of publication, which country the study performed, and the number of cases and controls for prohibitin genotypes (Table 1).…”

Genetic determinants of sporadic breast cancer in Sri Lankan women

“…Another study among North American women showed that the variant allele is associated with susceptibility to breast cancer in those aged 50 years and below who had a pedigree with a minimum of one affected first-degree relative [ 40 ]. However, case-control studies in Turkish and Australian women showed no association with breast cancer [ 41 , 42 ]. Its functional effect therefore needs further investigation to clearly identify its relationship with breast cancer in the Sri Lankan population.…”