Lack of association between <i><scp>HLA</scp>‐E</i> polymorphisms and transitional cell carcinoma of the bladder

Veiga‐Castelli, Luciana C.; Cruz, Athamy Sarah de Paula; Inácio, Moisés Morais; Mendes-Junior, Celso Teixeira; Vianello-Brondani, Rosana Pereira; Moreau, Philippe; Castelli, Erick C.; Donadi, Eduardo Antônio

doi:10.1111/tan.12159

Cited by 4 publications

(5 citation statements)

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“…However, this low nucleotide variability was in part believed to be a consequence of the small number of studies evaluating HLA-E variability by using DNA sequencing approaches. By taking into account the present and previous studies that used sequencing approaches (19,20,30), it became clear that HLA-E in fact deviates from the high diversity that is characteristic of the HLA classical loci. Considering Table 2, only 14 coding haplotypes were found.…”

Section: Discussionmentioning

confidence: 93%

“…The HLA‐E 3′UTR was defined in silico by aligning sequences AK292391, BC002578, NM_005516, X56841, NT_167249 and NT_113891.2. The region of interest (exon 1 through the 3′UTR up to nucleotide +4674) was amplified by a polymerase chain reaction (PCR), using a methodology described elsewhere . PCR was performed in a final volume of 50 µl containing 1.5 U of DNA polymerase Long (Fermentas, Vilnius, Lithuania) and 1.0× Long polymerase Buffer, 0.20 mM of each dNTP and 20 pmol of each primer.…”

Section: Methodsmentioning

confidence: 99%

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Worldwide HLA‐E nucleotide and haplotype variability reveals a conserved gene for coding and 3′ untranslated regions

et al. 2014

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The human leukocyte antigen-E (HLA-E) locus is a human major histocompatibility complex (MHC) gene associated with immune-modulation and suppression of the immune response by the interaction with specific natural killer (NK) and T cell receptors (TCRs). It is considered one of the most conserved genes of the human MHC; however, this low nucleotide variability seems to be a consequence of the scarce number of studies focusing on this subject. In this manuscript we assessed the nucleotide variability at the HLA-E coding and 3' untranslated regions (3'UTRs) in Brazil and in the populations from the 1000Genomes Consortium. Twenty-eight variable sites arranged into 33 haplotypes were detected and most of these haplotypes (98.2%) are encoding one of the two HLA-E molecules found worldwide, E*01:01 and E*01:03. Moreover, three worldwide spread haplotypes, associated with the coding alleles E*01:01:01, E*01:03:01 and E*01:03:02, account for 85% of all HLA-E haplotypes, suggesting that they arose early before human speciation. In addition, the low nucleotide diversity found for the HLA-E coding and 3'UTR in worldwide populations suggests that the HLA-E gene is in fact a conserved gene, which might be a consequence of its key role in the modulation of the immune system.

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Section: Discussionmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Worldwide HLA‐E nucleotide and haplotype variability reveals a conserved gene for coding and 3′ untranslated regions

et al. 2014

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“…One more study of nasopharyngeal carcinoma patients revealed no significant difference between both alleles [77]. No associations were found between HLA-E alleles and urinary bladder carcinoma [78].…”

Section: Hla-e Dimorphism and Cancermentioning

confidence: 99%

Dimorphism of HLA-E and Its Disease Association

Kanevskiy

Erokhina

Kobyzeva

et al. 2019

IJMS

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HLA-E is a nonclassical member of the major histocompatibility complex class I gene locus. HLA-E protein shares a high level of homology with MHC Ia classical proteins: it has similar tertiary structure, associates with β2-microglobulin, and is able to present peptides to cytotoxic lymphocytes. The main function of HLA-E under normal conditions is to present peptides derived from the leader sequences of classical HLA class I proteins, thus serving for monitoring of expression of these molecules performed by cytotoxic lymphocytes. However, opposite to multiallelic classical MHC I genes, HLA-E in fact has only two alleles—HLA-E*01:01 and HLA-E*01:03—which differ by one nonsynonymous amino acid substitution at position 107, resulting in an arginine in HLA-E*01:01 (HLA-ER) and glycine in HLA-E*01:03 (HLA-EG). In contrast to HLA-ER, HLA-EG has higher affinity to peptide, higher surface expression, and higher thermal stability of the corresponding protein, and it is more ancient than HLA-ER, though both alleles are presented in human populations in nearly equal frequencies. In the current review, we aimed to uncover the reason of the expansion of the younger allele, HLA-ER, by analysis of associations of both HLA-E alleles with a number of diseases, including viral and bacterial infections, cancer, and autoimmune disorders.

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“…The complete HLA-E gene was amplified in a single amplicon encompassing the entire transcribed segment (including the 5 0 and 3 0 untranslated regions), from nucleotides 30,457,191 (À118 according to IMGT/HLA and considering the Adenine of the first translated ATG as +1) to 30,462,211 (downstream the HLA-E 3 0 UTR) considering chromosome 6 from the human genome assembly hg19, by using the primers HE01F (5 0 -TCCTGGATACTCA TGACGCAGACTC-3 0 ) and HE3UTR.R1 (5 0 -GGACTCCCTGGGCTTTCTC ACCG-3 0 ), as described elsewhere [16,26]. According to the hg19 annotation, the HLA-E transcription starts at 30,457,183, the translation starts at 30,457,309 and the transcription ends at 30,461,982.…”

Section: Amplification Library Preparation and Sequencingmentioning

confidence: 99%

“…A major limitation of previous studies is that they sequenced only a few exons or used genotyping methods to detect known HLA-E polymorphisms [14,17,18,[23][24][25][26][27][28][29][30]. Even in the Brazilian population [18], which represents the most comprehensively studied sample for HLA-E at the moment, only exons 1-4, in addition to the 3 0 UTR were sequenced.…”

Section: Introductionmentioning

confidence: 99%

HLA-E coding and 3′ untranslated region variability determined by next-generation sequencing in two West-African population samples

et al. 2015

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Lack of association between HLA‐E polymorphisms and transitional cell carcinoma of the bladder

Cited by 4 publications

References 26 publications

Worldwide HLA‐E nucleotide and haplotype variability reveals a conserved gene for coding and 3′ untranslated regions

Worldwide HLA‐E nucleotide and haplotype variability reveals a conserved gene for coding and 3′ untranslated regions

Dimorphism of HLA-E and Its Disease Association

HLA-E coding and 3′ untranslated region variability determined by next-generation sequencing in two West-African population samples

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