Lack of Association Between PCK1 Polymorphisms and Obesity, Physical Activity, and Fitness in European Youth Heart Study (EYHS)

Abstract

Phosphoenolpyruvate carboxykinase‐1 (PCK1) is the rate‐limiting enzyme in the hepatic gluconeogenic pathway. Studies have shown that overexpression of Pck1 in mice results in obesity‐related traits and higher levels of physical activity (PA). Therefore, our aims were to investigate whether common genetic variation in the PCK1 gene influences obesity‐related traits, PA, and fitness, and to examine whether PA and fitness attenuate the influence of the PCK1 polymorphisms on obesity in children. Analyses were undertaken on data from Danish and Estonian children (958 boys and 1,104 girls) from the European Youth Heart Study (EYHS), a school‐based, cross‐sectional study of children (mean ± s.d. age: 9.6 ± 0.4 years) and adolescents (15.5 ± 0.5 years). We genotyped eight polymorphisms that captured the common genetic variations in the PCK1 gene. The association between the PCK1 polymorphisms and BMI, waist circumference (WC), sum of four skinfolds, PA, and fitness was tested using an additive model adjusted for age, age‐group, gender, maturity, and country. Interactions were tested by including interaction terms in the model. None of the polymorphisms were significantly associated with BMI, WC, sum of four skinfolds, PA, and fitness, and also with the risk of being overweight or obese (P > 0.05). The interactions between the polymorphisms and age‐group, gender, PA, and fitness were not statistically significant. This is the first study to comprehensively examine the association of PCK1 polymorphisms with obesity, PA, and fitness. Despite strong evidence from animal studies, our study in the EYHS cohort failed to identify an association of PCK1 polymorphisms with obesity, PA, and fitness.

Differentially expressed genes in the caecal and colonic mucosa of Landrace finishing pigs with high and low food conversion ratios

“…Phosphoenolpyruvate carboxykinase-1 (PCK1) was reported to participate in lipid metabolism 47 , is the rate-limiting enzyme in hepatic gluconeogenic pathways, and is closely related to obesity 48 . PCK1was also involved in pathways of glucose metabolism and pyruvate metabolism.…”

Identification of novel prognostic targets in acute kidney injury using bioinformatics and next generation sequencing data analysis

“…IGF2 [632], RPPH1 [633], PRKCB (protein kinase C beta) [634], CCL5 [635], VASH2 [636], GREM1 [637], CYP11B2 [638], HIC1 [639], PCK1 [640], HSD11B2 [641], MME (membrane metalloendopeptidase) [642], FABP1 [142], MIOX (myo-inositol oxygenase) [643], ARG2 [644], PPARGC1A [645], VNN1 [646], NOX4 [647], EPHX2 [577], DDIT4 [648], SLC2A1 [649], PFKFB2 [650], CDH2 [651], SLC22A2 [294], AQP2 [652], ANGPT1 [653], KL (klotho) [654], ACE2 [655], STC1 [656], REN (renin) [608], ERRFI1 [657], ERBB4 [658], NTNG1 [659], VCAM1 [660], PTGER3 [661] and BBOX1 [662] expression levels are associated with diabetic nephropathy. IGF2 [663], IRF7 [664], PRKCB (protein kinase C beta) [665], CCL5 [666], ACTN3 [667], AMH (anti-Mullerian hormone) [668], E2F1 [669], UBE2M [670], TP73 [671], AGER (advanced glycosylation end-product specific receptor) [672], SMPD3 [118], NR2E1 [673], ANGPTL3 [674], CYP3A5 [675], PCK1 [676], LRP2 [677], FABP1 [678], SLC22A12 [279], CYP8B1 [679], MIOX (myo-inositol oxygenase) [680], ARG2 [681], FGF1 [450], CRY1 [682], PPARGC1A [451], CRYM (crystallin mu) [683], SLC19A3 [283], FH (fumaratehydratase) [684], SLC2A9 [286], GC (GC vitamin D binding protein) [685], RGN (regucalcin) [686], MMAA (metabolism of cobalamin associated A) [687], NOX4 [688], ABHD6 [689], EPHX2 [690], DDIT4 [691], EGF (epidermal growth factor) [173], DEFB1 [692], ACE2 ...…”

Polymorphisms analysis for association between ADIPO signaling pathway and genetic susceptibility to T2DM in Chinese han population

“…Any abnormality in the kinase product produced at the transcriptional or translational level may lead to diabetes. Vimaleswaran [ 36 ] et al have found that PCK1 gene polymorphism is not associated with obesity in European adolescents. Rees [ 37 ]et al have discovered that rs1042531 is not associated with T2DM in South Asian populations.…”