Lack of association between genotypes and virulence factors in C. albicans strains isolated from vaginal secretion

Abstract

The physiopathogenesis of vulvovaginal candidiasis (VVC) is still not completely elucidated. The objective of this study was to evaluate if there is a relationship between the different genotypes of Candida albicans, their main agent and the virulence of this yeast in vaginal isolates, and to check if there are laboratorial markers that can predict the ability of each isolate to develop VVC independently of symptoms. The production of exoenzymes protease, phospholipase and haemolysin, resistance to hydrogen peroxide, and the genotype were determined. Genotype A was predominant (75%), protease, phospholipase and haemolytic activity were highly expressed, and the majority of the yeasts were sensitive to H2O2 in 1 and 2 hours of exposure, suggesting that these factors are important in the virulence of vaginal isolates. However they did not have any correlation with the genotypes. The different isolates expressed similar virulence potential, suggesting that other factors relating to the yeasts and the host must participate in the development of the clinical disease.

Increased expression of virulence attributes in oral Candida albicans isolates from human immunodeficiency virus-positive individuals

“…However, we could not establish a relationship between the genotypes and the virulence factors, or the genotypes and clinical infection. Earlier studies have reported similar findings (Shinobu et al, 2007;Abaci, 2011). …”

“…The results showed that genotype A was predominant in isolates from HIV-infected as well as -uninfected individuals. A higher incidence of genotype A among Candida genotypes has been reported previously (Sugita et al, 2002;Shinobu et al, 2007). However, we could not establish a relationship between the genotypes and the virulence factors, or the genotypes and clinical infection.…”

Phenotypes characterization and ABC genotypes distribution of clinical Candida albicans isolates

“…In a similar study, Gharaghani et al reported genotype C (83.5%) as the most common genotype in vulvovaginal samples and those data were similar to our study 32 . In contrast with the published data of Shinobu et al, genotype A is the most common genotype in vulvovaginal samples 33 . Among the reports, the ratios of genotypes A, B, and C of C. albicans isolates varied.…”

A Challenge for Clinical Laboratories: Detection of Antifungal Resistance inCandidaSpecies Causing Vulvovaginal Candidiasis

“…These yeasts were isolated and identified in 2008 through classical 25 and molecular 26 methods and are part of a yeast bank maintained in the Medical Mycology Division of the Laboratory of Teaching and Research in Clinical Analysis at the State University of Maringá, Paraná, Brazil. The isolates have been stored in Sabouraud Dextrose Broth (SDB) (Difco, Detroit, MI) with 10% glycerol at -20°C since their identification.…”