Lacisediminihabitans profunda gen. nov., sp. nov., a member of the family Microbacteriaceae isolated from freshwater sediment

Zhuo, Ya; Jin, Chun-Zhi; Jin, Feng-Jie; Li, Taihua; Kang, Dong Hyo; Oh, Hee‐Mock; Lee, Hyung-Gwan; Jin, Long

doi:10.1007/s10482-019-01347-8

Cited by 10 publications

(4 citation statements)

References 57 publications

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“…Amplification of the 16S rRNA gene was done with the PCR method and applying the universal primer set 27F/1492R [14]. The PCR amplification conditions were set up as follows: 95 °C for 5 min, 30 cycles of 95 °C for 1.5 min, 55 °C for 1 min and 72 °C for 1 min and final extension for 10 min at 72 °C [15]. To get the full 16S rRNA gene, sequencing was carried out with following primers: 27F (5′-AGAGTTTGATCCTGGCTCAG-3′), 785F (5′-GGATTAGATACCCTGGTA-3′), 800R (5′-TACCAGGGTATCTAATCC-3′) and 1492R (5′-GGTTACCTTGTTACGACTT-3′) [14, 16].…”

Section: Methodsmentioning

confidence: 99%

“…The antibiotic susceptibility of strain BN140002 T was checked on R2A agar plates using the paper disc method. The discs contained following antibiotics (μg per disc): nalidixic acid (30), tetracycline (30), amikacin (30), ampicillin/ sulbactam (20), kanamycin (30), vancomycin (30), chloramphenicol (30), teicoplanin (30), streptomycin (25), gentamicin (30), spectinomycin (25), rifampicin (30), lincomycin (15) and erythromycin (30). Other physiological and enzyme activities were assayed using API 20E, API ZYM and API 50CH strips (bioMérieux) according to the manufacturer's instructions.…”

Section: Morphological Physiological and Biochemical Characterizationmentioning

confidence: 99%

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Salinarimonas soli sp. nov., isolated from soil

Jin

Liu

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A light pink coloured bacterium, designated strain BN140002T, was isolated from a soil sample collected in Goesan-gun, Chungcheongbuk-do, Republic of Korea. Cells of strain BN140002T were Gram-stain-negative, aerobic, motile and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed 94.7, 94.7, 93.9, 93.3, 93.4 and 93.0% similarities to Salinarimonas rosea KCTC 22346T, Salinarimonas ramus DSM 22962T, Saliniramus fredricksonii HL-109T, Microvirga soli R491T, Chelatococcus caeni EBR-4-1T and Chelatococcus composti PC-2T, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine. The major cellular fatty acids were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) and summed feature 1 (C12 : 0 aldehyde and/or unknown 10.98) and the predominant ubiquinone was Q-10. The genomic DNA G+C content of strain BN140002T was 70.1 mol%. The genomic orthoANI values between strain BN140002T and Salinarimonas rosea KCTC 22346T and Salinarimonas ramus DSM 22962T were 75.0 and 74.8 %, respectively. Strain BN140002T had a class I-C type CRISPR-Cas system (CRISPR-associated helicase Cas3, CRISPR-associated protein Cas8c, CRISPR-associated protein Cas7, CRISPR-associated RecB family exonuclease Cas4, CRISPR-associated protein 1, 2). Based on phenotypic, chemotaxonomic and phylogenetic data, strain BN140002T should be assigned as a novel species of the genus Salinarimonas , for which the name Salinarimonas soli sp. nov. is proposed. The type strain is BN140002T (=KCTC 42643T=CCTCC AB 2017173T).

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Section: Methodsmentioning

confidence: 99%

Section: Morphological Physiological and Biochemical Characterizationmentioning

confidence: 99%

Salinarimonas soli sp. nov., isolated from soil

Jin

Liu

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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“…For analysis of cellular fatty acids, strain GW1-11 T and the reference strains were grown in TSA broth at 28 ℃ and cells were harvested in the late exponential phase (33). The fatty acids were prepared and identification was carried out according to the standard protocol of the Sherlock Microbial Identification System (34). Polar lipids and quinones were investigated from freeze-dried cells (35), and the polar lipids were identified by two-dimensional TLC as described.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Sporosarcina siccatus sp. nov., isolated from coastal sediment of the Great Wall Station, Antarctica

Mu,

Xie,

Jiang

et al. 2023

Preprint

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A Gram-stain-positive, aerobic and spherical bacterium, white, slightly convex colony with rounded edges, designated GW1-11T was isolated from the coastal sediment of the Great Wall Station, Antarctica. 16S rRNA gene sequence analysis showed that strain GW1-11T was closely related to Sporosarcina ureae DSM 2281T (94%). Growth was observed in the presence of 0–6.0%(w/v) NaCl, at pH 6.0–9.0, and at 4–37℃. Digital DNA-DNA relatedness between strain GW1-11T and its close relatives S. ureae DSM 2281T was 21.0%. The average nucleotide identity value between strain GW1-11T and S. ureae DSM 2281T was 77.9%. The strain GW1-11T possessed menaquinone-7 (MK-7) as the sole respiratory quinone. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The major fatty acids composition was summarized as C14: 0, C14:0 ω5c and iso-C14: 0. Based on phenotypic, chemotaxonomic characteristics, and phylogenetic data, strain GW1-11T represents a novel species of the genus Sporosarcina, for which the name Sporosarcina siccatus sp. nov., is proposed. The type strain is GW1-11T (= CCTCC AB 2019391T = KCTC43187T).

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“…At the time of writing, there are 59 validly published genera in the family Microbacteriaceae with Microbacterium as the type genus (https://lpsn.dsmz.de/family/chitinophagaceae). Members of the family Microbacteriaceae are Gram-stain-positive, predominantly aerobic, motile or non-motile, non-spore-forming, and have anteiso-C 15 : 0 , iso-C 16 : 0 and anteiso-C 17 : 0 as the major fatty acids [1–6]. During a study on the bacterial diversity in a sludge collected from a pesticide plant (located in Wenzhou, Zhejiang Province, PR China), a small, yellow-pigmented and smooth colony was screened and purified.…”

Section: Introductionmentioning

confidence: 99%

Ruicaihuangia caeni gen. nov., sp. nov., a novel taxon within the family Microbacteriaceae isolated from sludge

Liu,

Geng,

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-positive bacterium, designated YN-L-19T, was isolated from a sludge sample collected from a pesticide-manufacturing plant. Cells of YN-L-19T were strictly aerobic, non-spore-forming, non-motile and ovoid-shaped. Colonies were small, smooth and yellow. Growth occurred at 10–37 °C (optimum, 30 °C), pH 5.0–9.0 (optimum, 7.0) and 0–3.0 % (w/v) NaCl (optimum 0.5 %). Phylogenetic analysis based on genome and 16S rRNA gene sequences indicated that YN-L-19T was affiliated to the family Microbacteriaceae and most closely related to Diaminobutyricimonas aenilata, Terrimesophilobacter mesophilus, Planctomonas deserti and Curtobacterium luteum. The major cellular fatty acids of YN-L-19T were anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and C16 : 0. The predominant menaquinone was MK-7. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipid and one unidentified lipid. The average amino acid identity values between strain YN-L-19T and the related strains were 57.9–61.9 %, which were below the genus boundary (70 %). On the basis of the evidence presented in this study, strain YN-L-19T represents a novel species of a new genus in the family Microbacteriaceae, for which the name Ruicaihuangia caeni gen. nov., sp. nov. (type strain YN-L-19T=CCTCC AB 2022401T= KCTC 49935T) is proposed.

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Lacisediminihabitans profunda gen. nov., sp. nov., a member of the family Microbacteriaceae isolated from freshwater sediment

Cited by 10 publications

References 57 publications

Salinarimonas soli sp. nov., isolated from soil

Salinarimonas soli sp. nov., isolated from soil

Sporosarcina siccatus sp. nov., isolated from coastal sediment of the Great Wall Station, Antarctica

Ruicaihuangia caeni gen. nov., sp. nov., a novel taxon within the family Microbacteriaceae isolated from sludge

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