Lachnotalea glycerini gen. nov., sp. nov., an anaerobe isolated from a nanofiltration unit treating anoxic groundwater

Jarzembowska, Monika; Sousa, Diana Z.; Beyer, Florian; Zwijnenburg, A.; Plugge, Caroline M.; Stams, Alfons Johannes Maria

doi:10.1099/ijsem.0.000791

Cited by 14 publications

(3 citation statements)

References 20 publications

(14 reference statements)

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“…Cell morphology, motility and spore formation of strain L2-7 T was observed using a Leica DM 2000 microscope and a JEOL-6480LV scanning electron microscope (SEM). The sample processing for SEM was performed as previously described [14]. Strain L2-7 T is a non-motile and strictly anaerobic, straight rod-shaped organism.…”

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confidence: 99%

Reclassification of Eubacterium hallii as Anaerobutyricum hallii gen. nov., comb. nov., and description of Anaerobutyricum soehngenii sp. nov., a butyrate and propionate-producing bacterium from infant faeces

Shetty

Zuffa

Bui

et al. 2018

International Journal of Systematic and Evolutionary Microbiology

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A bacterial strain designated L2-7 T , phylogenetically related to Eubacterium hallii DSM 3353 T , was previously isolated from infant faeces. The complete genome of strain L2-7 T contains eight copies of the 16S rRNA gene with only 98.0-98.5 % similarity to the 16S rRNA gene of the previously described type strain E. hallii. The next closest validly described species is Anaerostipes hadrus DSM 3319 T (90.7 % 16S rRNA gene similarity). A polyphasic taxonomic approach showed strain L2-7 T to be a novel species, related to type strain E. hallii DSM 3353 T. The experimentally observed DNA-DNA hybridization value between strain L2-7 T and E. hallii DSM 3353 T was 26.25 %, close to that calculated from the genomes (34.3 %). The G+C content of the chromosomal DNA of strain L2-7 T was 38.6 mol%. The major fatty acids were C 16 : 0 , C 16 : 1 cis9 and a component with summed feature 10 (C 18 : 1 c11/t9/t6c). Strain L2-7 T had higher amounts of C 16 : 0 (30.6 %) compared to E. hallii DSM 3353 T (19.5 %) and its membrane contained phosphatidylglycerol and phosphatidylethanolamine, which were not detected in E. hallii DSM 3353 T. Furthermore, 16S rRNA gene phylogenetic analysis advocates that E. hallii DSM 3353 T is misclassified, and its reclassification as a member of the family Lachnospiraceae is necessary. Using a polyphasic approach, we propose that E. hallii (=DSM 3353 T =ATCC 27751 T) be reclassified as the type strain of a novel genus Anaerobutyricum sp. nov., comb. nov. and we propose that strain L2-7 T should be classified as a novel species, Anaerobutyricum soehngenii sp. nov. The type strain is L2-7 T (=DSM 17630 T =KCTC 15707 T).

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confidence: 99%

Reclassification of Eubacterium hallii as Anaerobutyricum hallii gen. nov., comb. nov., and description of Anaerobutyricum soehngenii sp. nov., a butyrate and propionate-producing bacterium from infant faeces

Shetty

Zuffa

Bui

et al. 2018

International Journal of Systematic and Evolutionary Microbiology

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“…Lachnotalea glycerini CCRI-19302 (GenBank accession number MF574095) has been isolated from a water sample harvested in Québec City, Canada. Comparative 16S rRNA gene sequence analysis showed an identity of 99.8% with the strain DLD10 T sequence (accession number MF953294) ( 1 ). Based on this criterion, strains CCRI-19302 and DLD10 T belong to the same species ( 2 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of a Sporulating and Motile Strain of Lachnotalea glycerini Isolated from Water in Québec City, Canada

et al. 2017

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“…Cell morphology, motility and spore formation of strain L2-7 was observed using a Leica DM 2000 microscope and a JEOL-6480LV scanning electron microscope (SEM). The sample processing for SEM was done as previously described (Jarzembowska, et al 2016). libraries at DSMZ, Braunschweig (Kuykendall, et al 1988, Kämpfer and Kroppenstedt 1996, Miller 1982).…”

Section: Introductionmentioning

confidence: 99%

Ecophysiological insights into the human intestinal microbiota: from single strains to defined consortia

Shetty¹

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High individuality, large complexity and limited understanding of the mechanisms underlying human intestinal microbiome function remain the major challenges for designing beneficial modulation strategies. Exemplified by the analysis of intestinal bacteria in a thousand Western adults, we discuss key concepts of the human intestinal microbiome landscape, i.e. the compositional and functional 'core', the presence of community types and the existence of alternative stable states. Genomic investigation of core taxa revealed functional redundancy, which is expected to stabilize the ecosystem, as well as taxa with specialized functions that have the potential to shape the microbiome landscape. The contrast between Prevotella-and Bacteroidesdominated systems has been well described. However, less known is the effect of not so abundant bacteria, for example, Dialister spp. that have been proposed to exhibit distinct bistable dynamics. Studies employing time-series analysis have highlighted the dynamical variation in the microbiome landscape with and without the effect of defined perturbations, such as the use of antibiotics or dietary changes. We incorporate ecosystem-level observations of the human intestinal microbiota and its keystone species to suggest avenues for designing microbiome modulation strategies to improve host health.

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Lachnotalea glycerini gen. nov., sp. nov., an anaerobe isolated from a nanofiltration unit treating anoxic groundwater

Cited by 14 publications

References 20 publications

Reclassification of Eubacterium hallii as Anaerobutyricum hallii gen. nov., comb. nov., and description of Anaerobutyricum soehngenii sp. nov., a butyrate and propionate-producing bacterium from infant faeces

Reclassification of Eubacterium hallii as Anaerobutyricum hallii gen. nov., comb. nov., and description of Anaerobutyricum soehngenii sp. nov., a butyrate and propionate-producing bacterium from infant faeces

Draft Genome Sequence of a Sporulating and Motile Strain of Lachnotalea glycerini Isolated from Water in Québec City, Canada

Ecophysiological insights into the human intestinal microbiota: from single strains to defined consortia

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