Lachancea

Lachancea quebecensis sp. nov., a yeast species consistently isolated from tree bark in the Canadian province of Québec

“…These data support the conclusion that the four strains reported here, are related to four previously reported strains: two from Canada, isolated from Prunus serotina in St. Anicet, Québec (UWOPS 79-139) and Drosophila in Pinery Provincial Park, Ontario (UWOPS 82-231) as well as two from Japan, isolated from deciduous leaves (NBRC 10066) and flowers (NBRC 10067). Prior studies stated that these four strains likely represent an undescribed species (Naumova et al, 2005(Naumova et al, , 2007Lachance & Kurtzman, 2011). Indeed, our data indicate that we have identified additional members of this lineage, and demonstrate that the PFGE profile is a relatively quick and affordable method to distinguish this species.…”

“…Among the strains identified were four isolates of the genus Lachancea that were initially considered to be L. thermotolerans. Further analysis revealed that in fact, these strains are related to isolates previously identified as a distinct population of L. thermotolerans, which share identical D1/D2 sequences, unique electrophoretic karyotypes and the ability to assimilate melibiose (Naumova et al, 2005(Naumova et al, , 2007Lachance & Kurtzman, 2011). Additionally, previous work highlighted that both isolate UWOPS 79-139 and isolate UWOPS 82-231 have atypical isoenzymic profiles, perhaps the first indication that they belong to a distinct lineage (Sidenberg & Lachance, 1986).…”

“…These sequences labelled 'Lachancea sp.' in the phylogeny, are identified by their respective strain IDs and GenBank accession numbers, and were previously included in a summary of the diversity in the genus Lachancea (Lachance & Kurtzman, 2011). The curated alignments of sequences from the novel species using 546 bp for the variable D1/D2 region of the 26S rRNA, was then used to reconstruct a phylogenetic tree using the neighbour-joining (Saitou & Nei, 1987) as well as maximum-likelihood methods (based on 1000 bootstrap iterations in both instances) with MEGA6 software (Tamura et al, 2013).…”

“…Additionally, previous work highlighted that both isolate UWOPS 79-139 and isolate UWOPS 82-231 have atypical isoenzymic profiles, perhaps the first indication that they belong to a distinct lineage (Sidenberg & Lachance, 1986). This group of divergent strains is represented by UWOPS 79-139, isolated from black knot on a cherry tree in St. Anicet in Québec, Canada (Sidenberg & Lachance, 1986), and has been suggested to be a novel species of the genus Lachancea (Naumova et al, 2007, Lachance & Kurtzman, 2011. The other three isolates in this previously described population include two from Japan and one from Pinery Provincial Park in Ontario, Canada.…”

“…The genus Lachancea represents an interesting group of closely related species that is an ideal model system for inter-and intra-specific diversity studies, as they constitute a range of phylogenetic relationships at different evolutionary distances. The species L. thermotolerans is particularly interesting as it was previously found to include isolates representing divergent populations (Naumova et al, 2005(Naumova et al, , 2007Lachance & Kurtzman, 2011).…”