Laccase production and simultaneous decolorization of synthetic dyes in unique inexpensive medium by new isolates of white rot fungus

Mishra, Abha; Kumar, Sudhir; Pandey, Anand Kumar

doi:10.1016/j.ibiod.2011.01.011

Cited by 46 publications

(30 citation statements)

References 29 publications

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“…Laccase activity: The method described by Mishra et al [8] has been slightly modified to characterize the presence of laccase activity on the solid medium. The solid culture medium contained 0.01% (w/v) of guaiacol (orthomethoxy phenol).…”

Section: Ligninolytic Enzymatic Activities On Solid Mediummentioning

confidence: 99%

“…as well as the fungal type, affect the decolorization of dyes. It is in this context that many studies have been interested in the optimization of the physicochemical parameters of the medium for new strains with high degradation abilities [7][8][9]. The Pycnoporus sanguineus (P. sanguineus) fungus is known for its ability to degrade lignin and synthetic molecules using ligninolytic enzymes [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Attéké

Mounguengui²,

Jean-Bosco

et al. 2013

J Bioremed Biodeg

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The fungus Pycnoporus sanguineus MUCL 51321 white rot isolated in Gabon showed a high ability to decolorize and degrade two synthetic dyes. On solid and liquid media, the fungus had different enzyme activities (laccase and manganese (Mn) peroxidase) in the presence of different substrates. At concentrations of 0.05 g/L (Orange G) and 0.3 g/L (reactive blue 4), the respective rates of decolorization of 81% and 97% were observed after 15 days of incubation in liquid media. In the same time interval, changes on the spectra (UV-vis and FTIR) and chromatograms (HPLC) showed that these decolorizations were due to the degradation of dyes by fungus with the production of new compounds. The study revealed the possibility of the use of this fungal strain in the microbial degradation process of synthetic dyes. Aldrich (Sigma-Aldrich, St. Louis, MO, USA). All reagents used were of the analytical grade. Journal of Bior emediation & Biodegradation Culture ConditionsThe composition of the solid growth medium used was as follows: 0.2% glucose, 0.2% malt extract, 0.01% MgSO 4 , 0.01% MnCl 2 , 0.03% NH 4 NO 3 , 0.026% KH 2 PO 4 , 0.026% Na 2 HPO 4 , 0.01% CaCl 2 .2H 2 O, 0.0001% FeC1 3 .6H 2 O, 0.0001% ZnCl 2 , 1.6% and 1.6% agar in 1 L of distilled water. The pH was adjusted to 5.5 with hydrochloric acid (0. 5 N) before autoclaving (at 121°C for 15 min). The culture medium was transferred into 4 Erlenmeyer flasks (200 mL each) and each reagent (enzymatic revelation) or dye, depending on its final concentration was added. The mixture was shaken, then about 15 mL were distributed in a Petri dish (diameter 8.5 mm); each test was replicated three times. 50 mL of liquid culture media in flasks of 100 mL were constituted in almost the same way as the solid culture media described above, except for the absence of agar. Each test was conducted three times. Decolorization experimentsDyes decolorization on solid medium: Solid culture media contained different concentrations of each dye (0.05 g/L to 1.5 g/L). Various controls were also prepared: some Petri dishes with fungus alone and others with dye alone to assess possible abiotic decolorization. One square portion of agar (1 cm of diameter) containing the fungus was placed at the centre of each Petri dish. The fungal growth diameters and the halos of decolorization were measured every day during one week at constants temperature (30°C) and humidity (75%). After these measures, different ratios and rates (decolorization or inhibition) were determined as described by . The optimum temperature of decolorization was evaluated using different growth temperatures (15, 22, 30, 35, 40 and 45°C). Moreover, the optimal concentrations for decolorization (0.05 g/L for the Orange G and 0.3 g/L for the RB4) were obtained after one week. All tests were conducted three times (averages are presented with the corresponding standard deviations).Dyes decolorization on liquid culture medium: For each Erlenmeyer flask (100 mL) containing liquid culture medium (50 mL) and dye (0.05 g/L Orange G or 0.3...

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Section: Ligninolytic Enzymatic Activities On Solid Mediummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Attéké

Mounguengui²,

Jean-Bosco

et al. 2013

J Bioremed Biodeg

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“…This hypsochromic shift of the spectral band accounts for the loss of conjugation in the double bonds. The Methyl orange degradation pathway has already been described (Telke et al, 2010;Mishra et al, 2011), thus it is not the subject of our work. Recently Telke et al (2010) demonstrated that the first step in the decolorization of azo dyes using laccase of Aspergillus ochraceus was the formation of an electronedeficient reaction center (carbocation).…”

Section: In Vitro Decolorization Of Dyes By Culture Fluids Of T Trogmentioning

confidence: 99%

Efficient azoic dye degradation by Trametes trogii and a novel strategy to evaluate products released

Levin

Grassi

Carballo

2012

International Biodeterioration & Biodegradation

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“…Nevertheless these processes are not always effective and economic. This has prompted the use of various materials as adsorbents in order to develop cheaper alternatives by utilizing many types of biosorbents as fungi (Kaushik and Malik, 2009;Mishra et al, 2011), bacteria (Banat et al, 1996;Yang et al, 2011) and yeasts (Ertugrul et al, 2009;Phugare et al, 2010). As well, one of the growing interest and promising biosorbents is "algae" (Veglio and Beolchini, 1997;Pengthamkeerati et al, 2008;Kousha et al, 2012) due to its high sorption capacity and its availability in almost unlimited amounts (Klimmek et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of non-viable biomass of Laurencia papillosa for decolorization of dye waste water

Maghraby¹

2013

Afr. J. Biotechnol.

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The uptake of fast orange dye by the red seaweed Laurencia papillosa has been demonstrated in order to explore its potential use as low-cost adsorbent. The adsorption kinetics of fast orange dye on the alga with respect to initial dye concentration, contact time, particle size and pH were investigated. The dye removal percentage increased from 25.92 to 67.08% and the equilibrium states were attained at almost 60 min within the experimental concentration range. The adsorption kinetic was analyzed using pseudo-first-order and pseudo-second-order models. The pseudo-second-order model was more appropriate to describe the sorption kinetics based on the relatively high values of the linear squared regression correlation coefficient. The nature of the possible adsorbent and fast orange interactions was examined by the Fourier transform infrared technique. This technique confirmed that hydroxyl, carboxyl, amine, sulfonyl, carbonyl and alkyl groups are responsible for the dye binding process. Significant increase in dye adsorption was observed with the decrease in sorbent particle size coupled with its large surface area. Maximum removal efficiency was determined to be 65.7% at a solution pH of 5. However, Laurencia papillosa proved to be a promising material for removing fast orange dye from aqueous solutions.

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Laccase production and simultaneous decolorization of synthetic dyes in unique inexpensive medium by new isolates of white rot fungus

Cited by 46 publications

References 29 publications

Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Biodegradation of Reactive Blue 4 and Orange G by Pycnoporus sanguineus Strain Isolated in Gabon

Efficient azoic dye degradation by Trametes trogii and a novel strategy to evaluate products released

Evaluation of non-viable biomass of Laurencia papillosa for decolorization of dye waste water

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