Laccase immobilized on chitosan-coated Fe3O4 nanoparticles as reusable biocatalyst for degradation of chlorophenol

Zhang, Kai; Yang, Wenzhong; Liu, Ying; Zhang, Kegui; Chen, Yun; Yin, Xingtian

doi:10.1016/j.molstruc.2020.128769

Cited by 78 publications

(30 citation statements)

References 40 publications

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“…Similar storage stability improvements for immobilized laccase compared to free laccase were previously reported [ 29 ]. The pH of the local environment affect desired application of the enzyme [ 30 ]. Hence, the pH stability of HNTs-M-chitosan (1%)-GTA- Laccase is very important.…”

Section: Resultsmentioning

confidence: 99%

Chitosan-Grafted Halloysite Nanotubes-Fe3O4 Composite for Laccase-Immobilization and Sulfamethoxazole-Degradation

et al. 2020

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A surface-engineered nano-support for enzyme laccase-immobilization was designed by grafting the surface of halloysite nanotubes (HNTs) with Fe3O4 nanoparticles and chitosan. Herein, HNTs were magnetized (HNTs-M) by a cost-effective reduction-precipitation method. The synthesized HNTs-M were grafted with 0.25%, 0.5%, 1%, and 2% chitosan (HNTs-M-chitosan), respectively. Synthesized HNTs-M-chitosan (0.25%), HNTs-M-chitosan (0.5%), HNTs-M-chitosan (1%) and HNTs-M-chitosan (2%) were linked with glutaraldehyde (GTA) for laccase immobilization. Among these formulations, HNTs-M-chitosan (1%) exhibited the highest laccase immobilization with 95.13% activity recovery and 100.12 mg/g of laccase loading. The optimized material was characterized thoroughly by scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HR-TEM), X-ray powder diffraction (XRD), thermal gravimetric analysis (TGA), and vibrating sample magnetometer (VSM) analysis. The immobilized laccase (HNTs-M-chitosan (1%)-GTA-Laccase) exhibited higher pH, temperature, and storage stabilities. The HNTs-M-chitosan (1%)-GTA-Laccase possesses excellent reusability capabilities. At the end of 10 cycles of the reusability experiment, HNTs-M-chitosan (1%)-GTA-Laccase retained 59.88% of its initial activity. The immobilized laccase was utilized for redox-mediated degradation of sulfamethoxazole (SMX), resulting in 41%, 59%, and 62% degradation of SMX in the presence of 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), guaiacol (GUA), and syringaldehyde (SA), respectively. Repeated SMX degradation (57.10% after the sixth cycle) confirmed the potential of HNTs-M-chitosan (1%)-GTA-Laccase for environmental pollutant degradation. Thus, we successfully designed chitosan-based, rapidly separable super-magnetic nanotubes for efficacious enhancement of laccase biocatalysis, which can be applied as nano-supports for other enzymes.

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Section: Resultsmentioning

confidence: 99%

Chitosan-Grafted Halloysite Nanotubes-Fe3O4 Composite for Laccase-Immobilization and Sulfamethoxazole-Degradation

et al. 2020

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“…Hence, the detailed activity recovery and laccase loading capacity data provided the information about immobilization behavior of α-Cellulose-Fe3O4-CTNs-SH. The significant laccase loading obtained by α-Cellulose-Fe3O4-CTNs-SH was quite high among the recently reported materials [33,46,[54][55][56][57][58][59][60][61]. Thus, the developed α-cellulose based material has significant potential to look like an enzyme immobilization material.…”

Section: Activity Recovery and Laccase Loading Capacity Evaluationsmentioning

confidence: 92%

α-Cellulose Fibers of Paper-Waste Origin Surface-Modified with Fe3O4 and Thiolated-Chitosan for Efficacious Immobilization of Laccase

et al. 2021

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The utilization of waste-paper-biomass for extraction of important α-cellulose biopolymer, and modification of extracted α-cellulose for application in enzyme immobilization can be extremely vital for green circular bio-economy. Thus, in this study, α-cellulose fibers were super-magnetized (Fe3O4), grafted with chitosan (CTNs), and thiol (-SH) modified for laccase immobilization. The developed material was characterized by high-resolution transmission electron microscopy (HR-TEM), HR-TEM energy dispersive X-ray spectroscopy (HR-TEM-EDS), X-ray diffraction (XRD), vibrating sample magnetometer (VSM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FT-IR) analyses. Laccase immobilized on α-Cellulose-Fe3O4-CTNs (α-Cellulose-Fe3O4-CTNs-Laccase) gave significant activity recovery (99.16%) and laccase loading potential (169.36 mg/g). The α-Cellulose-Fe3O4-CTNs-Laccase displayed excellent stabilities for temperature, pH, and storage time. The α-Cellulose-Fe3O4-CTNs-Laccase applied in repeated cycles shown remarkable consistency of activity retention for 10 cycles. After the 10th cycle, α-Cellulose-Fe3O4-CTNs possessed 80.65% relative activity. Furthermore, α-Cellulose-Fe3O4-CTNs-Laccase shown excellent degradation of pharmaceutical contaminant sulfamethoxazole (SMX). The SMX degradation by α-Cellulose-Fe3O4-CTNs-Laccase was found optimum at incubation time (20 h), pH (3), temperatures (30 °C), and shaking conditions (200 rpm). Finally, α-Cellulose-Fe3O4-CTNs-Laccase gave repeated degradation of SMX. Thus, this study presents a novel, waste-derived, highly capable, and super-magnetic nanocomposite for enzyme immobilization applications.

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“…The covalently bound laccase was stable and able to remove 2, 4-Dichloro-Phenol (2, 4-DCP) and 4-Choloro-Phenol (4-CP) effectively even up to 10 cycles. The breakdown of 4-CP and 2,4-DCP reached 75.5% and 91.4% after 12 h (Zhang et al, 2020). In another experiment, Li et al (2020) used Fe 3 O 4 core and chelated Cu 2+ of carbon shell to immobilize laccase.…”

Section: Nanotechnology and Enzyme Technologymentioning

confidence: 99%

“…Nanotechnology has generated interest among researchers due to its beneficial effects, such as its large provided surface area, the capability of multiple uses, its stability at harsh conditions, easy and efficient manipulations in materials, increased interaction, and many more. The integration of microorganisms and enzymes with nanotechnology has provided a greener approach toward the management of industrial effluents (Dixit et al, 2020;Zhang et al, 2020). The risk associated with chemically synthesized nanoparticles can be minimized through the use of microorganisms.…”

Section: Future Perspective and Challengesmentioning

confidence: 99%

Microbial Nanotechnology for Bioremediation of Industrial Wastewater

Mandeep

Shukla

2020

Front. Microbiol.

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Pollutant removal from industrial effluents is a big challenge for industries. These pollutants pose a great risk to the environment. Nanotechnology can reduce the expenditure made by industries to mitigate these pollutants through the production of eco-friendly nanomaterials. Nanomaterials are gaining attention due to their enhanced physical, chemical, and mechanical properties. Using microorganisms in the production of nanoparticles provides an even greater boost to green biotechnology as an emerging field of nanotechnology for sustainable production and cost reduction. In this mini review, efforts are made to discuss the various aspects of industrial effluent bioremediation through microbial nanotechnology integration. The use of enzymes with nanotechnology has produced higher activity and reusability of enzymes. This mini review also provides an insight into the advantages of the use of nanotechnology as compared to conventional practices in these areas.

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Laccase immobilized on chitosan-coated Fe3O4 nanoparticles as reusable biocatalyst for degradation of chlorophenol

Cited by 78 publications

References 40 publications

Chitosan-Grafted Halloysite Nanotubes-Fe3O4 Composite for Laccase-Immobilization and Sulfamethoxazole-Degradation

Chitosan-Grafted Halloysite Nanotubes-Fe3O4 Composite for Laccase-Immobilization and Sulfamethoxazole-Degradation

α-Cellulose Fibers of Paper-Waste Origin Surface-Modified with Fe3O4 and Thiolated-Chitosan for Efficacious Immobilization of Laccase

Microbial Nanotechnology for Bioremediation of Industrial Wastewater

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