Laccase enzyme detoxifies hydrolysates and improves biogas production from hemp straw and miscanthus

Schroyen, Michel; Hulle, Stijn Van; Holemans, Sander; Vervaeren, Han; Raes, Katleen

doi:10.1016/j.biortech.2017.07.137

Cited by 39 publications

(13 citation statements)

References 29 publications

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“…Several procedures have been described for the removal of inhibitory compounds from LB hydrolyzates in order to limit their negative action on the fermentation [34]. Among these, the extraction with ethyl acetate [35], ion exchange [36], AC adsorption [37] or biological treatments with microorganisms and enzymes [38] can be referred. The treatment with AC is considered an effective method for the removal of phenolic compounds from LB hydrolyzates because of the large surface area of the charcoal particles, high adsorption capacity, and availability [36].…”

Section: Saccharification Of Cs and Detoxification With Activated Chamentioning

confidence: 99%

Development of an Energy Biorefinery Model for Chestnut (Castanea sativa Mill.) Shells

et al. 2017

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Chestnut shells (CS) are an agronomic waste generated from the peeling process of the chestnut fruit, which contain 2.7-5.2% (w/w) phenolic compounds and approximately 36% (w/w) polysaccharides. In contrast with current shell waste burning practices, this study proposes a CS biorefinery that integrates biomass pretreatment, recovery of bioactive molecules, and bioconversion of the lignocellulosic hydrolyzate, while optimizing materials reuse. The CS delignification and saccharification produced a crude hydrolyzate with 12.9 g/L of glucose and xylose, and 682 mg/L of gallic acid equivalents. The detoxification of the crude CS hydrolyzate with 5% (w/v) activated charcoal (AC) and repeated adsorption, desorption and AC reuse enabled 70.3% (w/w) of phenolic compounds recovery, whilst simultaneously retaining the soluble sugars in the detoxified hydrolyzate. The phenols radical scavenging activity (RSA) of the first AC eluate reached 51.8 ± 1.6%, which is significantly higher than that of the crude CS hydrolyzate (21.0 ± 1.1%). The fermentation of the detoxified hydrolyzate by C. butyricum produced 10.7 ± 0.2 mM butyrate and 63.9 mL H 2 /g of CS.Based on the obtained results, the CS biorefinery integrating two energy products (H 2 and calorific power from spent CS), two bioproducts (phenolic compounds and butyrate) and one material reuse (AC reuse) constitutes a valuable upgrading approach for this yet unexploited waste biomass.

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Section: Saccharification Of Cs and Detoxification With Activated Chamentioning

confidence: 99%

Development of an Energy Biorefinery Model for Chestnut (Castanea sativa Mill.) Shells

et al. 2017

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“…A negative linear correlation between lignin content and biodegradability was also observed. The inhibiting effect of lignin derivatives on methanogenesis of lignocellulosic biomass was recently reported by Schroyen et al [23], who found that phenolic compounds released from Miscanthus severely inhibited AD, thus lowering biogas production. Thus, the fact that, in the present study, VS removal efficiency was 1.5-times lower in R-MS than in R-ZM, and the observed methane yield was 33% lower than that which theoretically could be produced in these experimental conditions could be due to the inhibitory effect of M. sacchariflorus lignin.…”

Section: Biogas/methane Productionmentioning

confidence: 68%

Effect of Individual Components of Lignocellulosic Biomass on Methane Production and Methanogen Community Structure

Pokój

Klimiuk

Bułkowska

et al. 2018

Waste Biomass Valor

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One of the major factors that influences the economic feasibility of biogas production is the availability of digestible feedstocks. There is little research on the influence of the chemical composition of biomass on biogas synthesis, especially with regard to the content of lignocellulosic materials. Therefore, the aim of this study was to estimate how differences in the content of cellulose and lignin in lignocellulosic biomass influence the concentrations of individual volatile fatty acids (VFAs) and biogas production. Additionally, the structure of the methanogenic community was examined. The removal of fibrous and non-fibrous materials, the concentrations of individual VFAs, methane production and methanogen community structure were examined during digestion of Zea mays L. and Miscanthus sacchariflorus silages. Organics were removed with higher efficiency during the digestion of Z. mays silage than during digestion of M. sacchariflorus. This was due to the higher non-fibrous carbohydrates content in Z. mays than in M. sacchariflorus. In both digesters, propionate predominated throughout experiment. The methanogenic community in the digester fed with Z. mays was more diverse than that in the digester with M. sacchariflorus. Analysis of 16S rRNA sequences showed that six acetoclastic and four hydrogenotrophic methanogens were present in the digester fed with Z. mays L., while five acetoclastic and three hydrogenotrophic methanogens were in the digester fed with M. sacchariflorus. The abundance of Methanosarcina correlated significantly with the concentration of all analyzed VFAs.Keywords Crop silage · Anaerobic digestion · Syntrophic volatile fatty acids oxidation · Archaeal community structure · Methane yield Statement of NoveltyTo introduce novel biomass types for biogas production, an integrated evaluation approach has to be applied. The present study delivers a combination of technological and microbial data about the process of anaerobic digestion of the commonly used substrates Zea mays L. silage and Miscanthus sacchariflorus silage, the latter of which has a high content of lignin and cellulose. The correlations between the abundance of methanogenic microorganisms and the concentrations of individual VFAs produced during digestion of these substrates were estimated. The results reveal novel associations between the type of substrate used, the rate of methane production and the structure of the methanogenic community. The use of materials with a high lignin content limits biodiversity in the anaerobic digesters, which can result in decreases in anaerobic digestion performance and biogas production.

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“…In bioreactors containing wood, there was a significantly lower amount of biomethane production (P<0.05, Figures 5,6) in the enzyme-augmented reactors compared to the control. This effect was also found by Schroyen et al (Schroyen et al, 2017;Schroyen et al, 2014;Schroyen et al, 2015), where not all lignocellulosic substances responded positively to pre-treatment through peroxidase enzymes prior to anaerobic digestion, in some instances the treatment negatively impacted methane production (e.g. with corn stover, wheat straw, maize).…”

Section: Enzymatic Enhancementmentioning

confidence: 70%

“…Schroyen et al applied peroxidase enzymes to lignocellulosic materials (Schroyen et al, 2017;Schroyen et al, 2014;Schroyen et al, 2015). Interestingly, the case where they were able to obtain maximum enhancement of biogas from corn stover (4.5% lignin) was with a laccase treatment, not peroxidase or their mixture, and it resulted in a 17% increase in methane yield.…”

Section: General Discussion Of Bacterial and Enzyme Experimentsmentioning

confidence: 99%

“…Acceleration of breakdown of lignin-rich materials will require more rapid enzymatic activity, which can be addressed in two ways: (i) extracellular enzymes similar to those produced by white-rot fungi, which are available commercially, can be added to the system thereby eliminating the need to use the organism itself in biodelignification Hettiaratchi et al, 2015;Jayasinghe et al, 2011;Jayasinghe et al, 2014;Jayasinghe et al, 2013). Researchers have applied this technique of enzymatic biodelignification with the prospect of enhanced recovery of chemicals/biogas with varying degrees of success (Schroyen et al, 2017;Schroyen et al, 2014;Schroyen et al, 2015) although not to realistic, complex waste materials such as wood or newspaper. (ii) a second approach is the application of bacteria for delignification, this has received only very limited attention but is gaining popularity and shows promise in enhancing the breakdown of lignocellulosic wastes for various biotechnological applications (e.g.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Application of enzymatic and bacterial biodelignification systems for enhanced breakdown of model lignocellulosic wastes

Muaaz-Us-Salam

Cleall

Harbottle

2020

Science of The Total Environment

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Laccase enzyme detoxifies hydrolysates and improves biogas production from hemp straw and miscanthus

Cited by 39 publications

References 29 publications

Development of an Energy Biorefinery Model for Chestnut (Castanea sativa Mill.) Shells

Development of an Energy Biorefinery Model for Chestnut (Castanea sativa Mill.) Shells

Effect of Individual Components of Lignocellulosic Biomass on Methane Production and Methanogen Community Structure

Application of enzymatic and bacterial biodelignification systems for enhanced breakdown of model lignocellulosic wastes

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