Laccase-Assisted Degradation of Anticancer Drug Etoposide: By-Products and Cytotoxicity

Pereira, Cláudia Maria; Kelbert, Maikon; Daronch, Naionara Ariete; Cordeiro, Arthur Poester; Cesca, Karina; Michels, Camila; Oliveira, Débora de; Soares, Hugo Moreira

doi:10.1007/s12155-023-10604-8

Cited by 3 publications

(2 citation statements)

References 53 publications

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“…They have been identified in wastewater near hospitals at concentrations ranging from 0.02 to 0.025 µg/L [7], and their removal in WWTP is not consistently effective [8,[88][89][90]. The effectiveness of laccase was evaluated using doxorubicin and etoposide as model anticancer drugs [83][84][85].…”

Section: Cytostaticsmentioning

confidence: 99%

“…Its presence in wastewater poses a threat due to its potential carcinogenicity, mutagenicity, and genotoxicity [92]. Pereira et al [85] tested the ability of laccase from T. versicolor (Sigma-Aldrich) to remove this drug. Laccase with a high enzyme activity of 1100 U/L achieved 100% degradation within 1 h, while reducing the laccase activity to 55 U/L achieved 86% removal of etoposide within 6 h, with approximately 68% removal after 1 h. Dark-brown products after the enzyme-catalyzed reaction were determined via LC-MS-MS, with results indicating a biotransformation of the colorless drug via dehydration and demethylation.…”

Section: Cytostaticsmentioning

confidence: 99%

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Laccases as Effective Tools in the Removal of Pharmaceutical Products from Aquatic Systems

Chmelová,

Ondrejovič,

Miertuš

2024

Life

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This review aims to provide a comprehensive overview of the application of bacterial and fungal laccases for the removal of pharmaceuticals from the environment. Laccases were evaluated for their efficacy in degrading pharmaceutical substances across various categories, including analgesics, antibiotics, antiepileptics, antirheumatic drugs, cytostatics, hormones, anxiolytics, and sympatholytics. The capability of laccases to degrade or biotransform these drugs was found to be dependent on their structural characteristics. The formation of di-, oligo- and polymers of the parent compound has been observed using the laccase mediator system (LMS), which is advantageous in terms of their removal via commonly used processes in wastewater treatment plants (WWTPs). Notably, certain pharmaceuticals such as tetracycline antibiotics or estrogen hormones exhibited degradation or even mineralization when subjected to laccase treatment. Employing enzyme pretreatment mitigated the toxic effects of degradation products compared to the parent drug. However, when utilizing the LMS, careful mediator selection is essential to prevent potential increases in environment toxicity. Laccases demonstrate efficiency in pharmaceutical removal within WWTPs, operating efficiently under WWTP conditions without necessitating isolation.

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Section: Cytostaticsmentioning

confidence: 99%

Section: Cytostaticsmentioning

confidence: 99%

Laccases as Effective Tools in the Removal of Pharmaceutical Products from Aquatic Systems

Chmelová,

Ondrejovič,

Miertuš

2024

Life

View full text Add to dashboard Cite

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Industrial Biotechnology as a Multidisciplinary Tool for Developing Sustainable Technologies

Coelho,

Ribeiro,

de Oliveira

et al. 2023

Bioenerg. Res.

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Leveraging the Pharmaceutical Area Through Multidisciplinary Synergy

Razak,

Diba,

Xin

et al. 2024

Advances in Higher Education and Professional Development

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The environmental implications of pharmaceutical industry drawn the attention of environmental scientists, prompting multidisciplinary collaborations toward sustainable solutions. Driven by the urgent need to combat the environmental persistence of pharmaceuticals, scholars across diverse disciplines including pharmaceutical sciences, biotechnology and chemical engineering are collaborating to develop effective solutions. Biotechnology uses microbes' natural breakdown power, while chemical engineering creates ideal conditions for efficient drug removal. In this work, a transdisciplinary approach was employed to study a model system comprising ibuprofen and a laccase enzyme. By empowering researchers with a comprehensive understanding of multidisciplinary approaches, the project seeks to optimize reaction conditions for maximized drug degradation efficiency, contributing to environmental protection and cleaner water sources.

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Laccase-Assisted Degradation of Anticancer Drug Etoposide: By-Products and Cytotoxicity

Cited by 3 publications

References 53 publications

Laccases as Effective Tools in the Removal of Pharmaceutical Products from Aquatic Systems

Laccases as Effective Tools in the Removal of Pharmaceutical Products from Aquatic Systems

Industrial Biotechnology as a Multidisciplinary Tool for Developing Sustainable Technologies

Leveraging the Pharmaceutical Area Through Multidisciplinary Synergy

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