Laboratory Techniques of Studying Plant Chromosomes

Kashtwari, Mahpara; Zargar, Showkat Ali; Wani, Aijaz A.

doi:10.1007/978-81-322-3673-3_4

Cited by 4 publications

(4 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the microscopic examination of the nuclei, the staining of the genetic material with acetic orcein followed by the squash of the root tip was used. [ 38 ] The examination was performed under the 100X objective lens using cedar oil for immersion, and nuclei were observed for changes in the karyokinetic film.…”

Section: Methodsmentioning

confidence: 99%

Co (II), Cu (II), Mn (II), Ni (II), Pd (II), and Pt (II) complexes of bidentate Schiff base ligand: Synthesis, crystal structure, and acute toxicity evaluation

Lupașcu

Pahontu

Shova³

et al. 2021

Applied Organom Chemis

View full text Add to dashboard Cite

5‐methoxy‐2‐(((2chloro‐5‐(trifluoromethyl)phenyl)imino)methyl)phenol) (HL) and its cobalt (II), copper (II), manganese (II), nickel (II), palladium (II), and platinum (II) complexes, [Co(L)2]·4H2O (1), [Cu(L)2] (2), [Mn(L)2(H2O)2]·H2O (3), [Ni(L)2] (4), [Pd(L)2] (5), [Pt(L)2] (6), were synthesized and characterized. The compounds were investigated by different physico‐chemical techniques including IR, 1H‐NMR, 13C‐NMR, UV‐Vis, mass spectroscopies, elemental and thermal analysis, magnetic susceptibility measurements, and molar electric conductivity. The molecular structures of the HL ligand and copper (II), nickel (II), and palladium (II) complexes were confirmed by X‐ray crystallography analysis on the monocrystal. Each metal ion, in these complexes, is four‐coordinated in N2O2 coordination environment. The coordination geometry of Ni2+ and Pd2+ is square planar due to cystallographically imposed inversion symmetry of the metal centers, and the coordination geometry of copper atom can be described as tetrahedrally distorted square planar. The assessed toxicity of the ligand and its complex combinations on plant cell of Triticum aestivum L. were macroscopically and microscopically consistent. The same substances were investigated for the animal cell toxicity on crustacean Artemia franciscana Kellogg, and Mn2+ complex did not record any lethal effect.

show abstract

Section: Methodsmentioning

confidence: 99%

Co (II), Cu (II), Mn (II), Ni (II), Pd (II), and Pt (II) complexes of bidentate Schiff base ligand: Synthesis, crystal structure, and acute toxicity evaluation

Lupașcu

Pahontu

Shova³

et al. 2021

Applied Organom Chemis

View full text Add to dashboard Cite

show abstract

“…The root elongation was measured over 3 days as follows: at 24, 48, and 72 h. The microscopic analysis was performed after 24 h of contact with the test solutions on one of the 11 seeds. 0.5 cm root tips were stained with acetic orcein [ 59 ]. The appearance of the nucleus, cytoplasm, cell wall, and changes in mitotic film were tracked.…”

Section: Methodsmentioning

confidence: 99%

New Pyrrole Derivatives as Promising Biological Agents: Design, Synthesis, Characterization, In Silico, and Cytotoxicity Evaluation

Ivan

Bărbuceanu

Hotnog

et al. 2022

IJMS

View full text Add to dashboard Cite

The current study describes the synthesis, physicochemical characterization and cytotoxicity evaluation of a new series of pyrrole derivatives in order to identify new bioactive molecules. The new pyrroles were obtained by reaction of benzimidazolium bromide derivatives with asymmetrical acetylenes in 1,2-epoxybutane under reflux through the Huisgen [3 + 2] cycloaddition of several ylide intermediates to the corresponding dipolarophiles. The intermediates salts were obtained from corresponding benzimidazole with bromoacetonitrile. The structures of the newly synthesized compounds were confirmed by elemental analysis, spectral techniques (i.e., IR, 1H-NMR and 13C-NMR) and single-crystal X-ray analysis. The cytotoxicity of the synthesized compounds was evaluated on plant cells (i.e., Triticum aestivum L.) and animal cells using aquatic crustaceans (i.e., Artemia franciscana Kellogg and Daphnia magna Straus). The potential antitumor activity of several of the pyrrole derivatives was studied by performing in vitro cytotoxicity assays on human adenocarcinoma-derived cell lines (i.e., LoVo (colon), MCF-7 (breast), and SK-OV-3 (ovary)) and normal human umbilical vein endothelial cells (HUVECs). The obtained results of the cytotoxicity assessment indicated that the tested compounds had nontoxic activity on Triticum aestivum L., while on Artemia franciscana Kellogg nauplii, only compounds 2c and 4c had moderate toxicity. On Daphnia magna, 4b and 4c showed high toxicity; 2a, 2b, and 2c moderate to high toxicity; only 4a and 4d were nontoxic. The compound-mediated cytotoxicity assays showed that several pyrrole compounds demonstrated dose- and time-dependent cytotoxic activity against all tested tumor cell lines, the highest antitumor properties being achieved by 4a and its homologue 4d, especially against LoVo colon cells.

show abstract

“…For the microscopic examination of the nuclei, the staining of the genetic material with acetic orcein followed by the squash of the root tip was used. [ 30 ] The examination was performed under the 100 x objective lens using cedar oil for immersion, and nuclei were observed for changes in the karyokinetic film.…”

Section: Methodsmentioning

confidence: 99%

Synthesis, characterization, crystal structure and toxicity evaluation of Co (II), Cu (II), Mn (II), Ni (II), Pd (II) and Pt (II) complexes with Schiff base derived from 2‐chloro‐5‐(trifluoromethyl)aniline

Lupașcu

Pahontu

Shova³

et al. 2020

Applied Organom Chemis

View full text Add to dashboard Cite

A bidentate NO donor Schiff base, 2-(((2-chloro-5-(trifluoromethyl)phenyl) imino)methyl) phenol (HL 1) and its complexes [Co(L 1) 2 (H 2 O) 2 ] (1), [Cu(L 1) 2 ] (2), [Mn(L 1) 2 (H 2 O) 2 ] (3), [Ni(L 1) 2 (H 2 O) 2 ] (4), [Pd 2 (L 1) 2 (OAc) 2 Á1.16H 2 O] (5), [Pt (L 1) 2 ] (6) were synthesized and characterized by different physico-chemical techniques including elemental and thermal analysis, magnetic susceptibility measurements, molar electric conductivity, IR, 1 H-NMR, 13 C-NMR, UV-Vis, mass spectroscopies and X-ray powder diffraction (XRD). The molecular structures of ligand HL 1 and two complexes (2 and 5) were confirmed by X-ray crystallography analysis on the monocrystal. In this complexes, the metal ions are in distorted square-planar environments. The copper (II) complex is mononuclear and crystallized in a monoclinic space group P21/c, whereas palladium (II) complex is dinuclear and crystallized in the trigonal crystal system R-3. The toxicity of the ligand and complexes was evaluated on both plant and animal cells, using the plant species Triticum aestivum L. and the crustacean Artemia franciscana Kellogg. At concentrations up to 100 μM the compounds presented very little toxicity on Artemia franciscana Kellogg. Moreover, the palladium (II) complex was devoid of any toxicity on the plant cells.

show abstract

Laboratory Techniques of Studying Plant Chromosomes

Cited by 4 publications

References 75 publications

Co (II), Cu (II), Mn (II), Ni (II), Pd (II), and Pt (II) complexes of bidentate Schiff base ligand: Synthesis, crystal structure, and acute toxicity evaluation

Co (II), Cu (II), Mn (II), Ni (II), Pd (II), and Pt (II) complexes of bidentate Schiff base ligand: Synthesis, crystal structure, and acute toxicity evaluation

New Pyrrole Derivatives as Promising Biological Agents: Design, Synthesis, Characterization, In Silico, and Cytotoxicity Evaluation

Synthesis, characterization, crystal structure and toxicity evaluation of Co (II), Cu (II), Mn (II), Ni (II), Pd (II) and Pt (II) complexes with Schiff base derived from 2‐chloro‐5‐(trifluoromethyl)aniline

Contact Info

Product

Resources

About