Laboratory Techniques in Plant Bacteriology

Borkar, S. G.

doi:10.1201/9781315206882

Cited by 36 publications

(29 citation statements)

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“…The suspension was again mixed with three volumes of cold 96% ethanol and allowed to precipitate for 24 h at 4⁰C. After this second precipitation round, in all samples but Δeps, the EPS could be observed as a white gel floating at the ethanol/air interface 47 . The gel was carefully collected using a syringe, transferred to a fresh Eppendorf tube and pelleted down (1 min, 12000 × g).…”

Section: Eps Precipitationmentioning

confidence: 99%

Collapse of genetic division of labour and evolution of autonomy in pellicle biofilms

et al. 2018

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Closely related microbes often cooperate, but the prevalence and stability of cooperation between different genotypes remains debatable. Here, we track the evolution of pellicle biofilms formed through genetic division of labour and ask whether partially deficient partners can evolve autonomy.Pellicles of Bacillus subtilis rely on an extracellular matrix composed of exopolysaccharide (EPS) and the fibre protein TasA. In monocultures, ∆eps and ∆tasA mutants fail to form pellicles, but, facilitated by cooperation, they succeed in co-culture. Interestingly, cooperation collapses on an evolutionary timescale, and ∆tasA gradually outcompetes its partner ∆eps. Pellicle formation can evolve independently from division of labour in ∆eps and ∆tasA monocultures, by selection acting on the residual matrix component, TasA or EPS respectively. Using a set of interdisciplinary tools, we unravel that the TasA-producer (∆eps) evolves via an unconventional but reproducible substitution in TasA that modulates the biochemical properties of the protein. On the contrary, the EPS-producer (ΔtasA) undergoes genetically variable adaptations, all leading to enhanced EPS secretion and biofilms with different biomechanical properties. Finally, we revisit the collapse of division of labour between Δeps and ΔtasA in light of a strong frequency vs. exploitability trade-off that manifested in the solitarily evolving partners. We propose such trade-off differences may represent an additional barrier to evolution of division of labour between genetically distinct microbes.

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Section: Eps Precipitationmentioning

confidence: 99%

Collapse of genetic division of labour and evolution of autonomy in pellicle biofilms

et al. 2018

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“…Several biochemical tests and utilization of sugars are used for differentiation among bacterial species of the same genus. 9 Recently genomic variation among the species are also studied and reported, but sometimes there is very negligible genomic variation in gene sequence 3 and therefore biochemical utilization tests are still useful to differentiate among species of the same genus.…”

Section: Discussionmentioning

confidence: 99%

Differentiation tests between the plant pathogenic bacteria Klebsiella pneumoniae and K. variicola

Sg¹,

Ts²

2018

JABB

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“…2 The presence of these bacteria in vascular tissues is shown by string test. 1 However in pomegranate wilt the bacterial pathogen Klebsiella pneumoniae infect the root bark comprising of epidermal tissues and cortex and incite the root bark necrosis symptoms. Klebsiella pneumoniae does not infect the vascular tissues.…”

Section: Discussionmentioning

confidence: 99%

Histopathology of Klebsiella pneumoniae strain Borkar inciting root bark necrosis and wilt in pomegranate

Sg¹,

Ts²

2018

JABB

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Histopathology of root bark necrosis and wilt in pomegranate incited by Klebsiella pneumoniae strain Borkar revealed that the bacterial infection was non-vascular in nature. The bacteria infect the epidermal layer and cortex of the bark tissues which are easily peeled off. Due to absence of root bark, the absorption of water and food material is hindered which results in wilting of the pomegranate plant. All other plant pathogenic bacteria inducing wilting in other plants infect the vascular system of the plant which finally leads to plant death, however the Klebsiella pneumoniae strain Borkar inciting root bark necrosis and wilt in pomegranate differed from other plant pathogenic bacteria by infecting only the cortex and epidermal cells of root bark to induce wilting in infected pomegranate plants.

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Laboratory Techniques in Plant Bacteriology

Cited by 36 publications

References 0 publications

Collapse of genetic division of labour and evolution of autonomy in pellicle biofilms

Collapse of genetic division of labour and evolution of autonomy in pellicle biofilms

Differentiation tests between the plant pathogenic bacteria Klebsiella pneumoniae and K. variicola

Histopathology of Klebsiella pneumoniae strain Borkar inciting root bark necrosis and wilt in pomegranate

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