Laboratory studies on the biocontrol potential of the predatory nematode Koerneria sudhausi (Nematoda: Diplogastridae)

Bar-Eyal, M.; Sharon, Edna; Spiegel, Y.; Oka, Yuji

doi:10.1163/156854108785787271

Cited by 8 publications

(2 citation statements)

References 6 publications

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“…The following nematodes and laboratory strains were used in this study: C. elegans N2 ( C. elegans Genetics Center); P. pacificus PS312 ( Sommer et al 1996 ), A. sudhausi SB413 ( Bar-Eyal et al 2008 ; Fürst von Lieven 2008 ), and A. seani RS1982 ( Kanzaki et al 2015 ). Note that A. sudhausi was originally described as Koerneria sudhausi .…”

Section: Methodsmentioning

confidence: 99%

A New Hope: A Hermaphroditic Nematode Enables Analysis of a Recent Whole Genome Duplication Event

Wighard

Athanasouli

Witte

et al. 2022

Genome Biology and Evolution

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Whole genome duplication (WGD) is often considered a major driver of evolution that leads to phenotypic novelties. However, the importance of WGD for evolution is still controversial because most documented WGD events occurred anciently and few experimental systems amenable to genetic analysis are available. Here, we report a recent WGD event in the hermaphroditic nematode Allodiplogaster sudhausi and present a comparison with a gonochoristic (male/female) sister species that did not undergo WGD. Self-fertilizing reproduction of A. sudhausi make it amenable to functional analysis and an ideal system to study WGD events. We document WGD in A. sudhausi through karyotype analysis and whole genome sequencing, the latter of which allowed us to i) identify functional bias in retention of protein domains and metabolic pathways, ii) show most duplicate genes are under evolutionary constraint, iii) show a link between sequence and expression divergence and iv) characterize differentially expressed duplicates. We additionally show the WGD is associated with increased body size and an abundance of repeat elements (36% of the genome), including a recent expansion of the DNA-hAT/Ac transposon family. Finally, we demonstrate the use of CRISPR/Cas9 to generate mutant knock-outs, whereby two WGD-derived duplicate genes display functional redundancy in that they both need to be knocked out to generate a phenotype. Together, we present a novel experimental system that is convenient for examining and characterizing WGD-derived genes both computationally and functionally.

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Section: Methodsmentioning

confidence: 99%

A New Hope: A Hermaphroditic Nematode Enables Analysis of a Recent Whole Genome Duplication Event

Wighard

Athanasouli

Witte

et al. 2022

Genome Biology and Evolution

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“…Osman (1988), in a pot experiment, found that by adding predatory nematode, Diplogaster sp., the parasitic population of Tylenchulus semipenetrans or M. javanica in the roots of sour orange and tomato, respectively, were significantly reduced. Diplogasterid predator, Koerneria sudhausi cultured on bacteria, resulted in a reduced tomato root galling index when the predator was introduced into the M. javanica-infested soil in pots (Bar-Eyal et al 2008).…”

Section: Pot Studymentioning

confidence: 99%

Effect of prey density of meloidogyne incognita on the predation efficiency of the predator, fictor composticola (nematoda: diplogasteridae)

Sidhu

Kanwar

2020

Egypt J Biol Pest Control

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Predatory nematodes are important as they fed upon several prey nematode species. The diplogasterid predators are characterized by distinguished alterations in feeding apparatus. The diplogasterid predator, Fictor composticola, feeds on different categories of nematodes like microbivorous, mycophagous, and plant-parasitic nematodes. This study was undertaken under in-vitro conditions, in 1% water-agar plates, and in pots under screen house. In lab. study, 250, 500, 1000, 2000 freshly hatched juveniles of Meloidogyne incognita, and 2 females and 2 males of F. composticola were released in each plate. Observations on prey consumed were recorded after 24 and 48 hrs. of deliverance. Prey consumption of F. composticola boosted as the number of prey increased from 250 to 2000 per plate. At prey density of 500 per plate, maximum per cent consumption was observed and minimum at prey density 2000 per plate. Maximum predation found at a prey population density of 2000 individuals and minimum in 250 individuals per plate. This finding led to the conclusion of increased contacts between prey and predator at higher densities. In the pot experiment, plant height and shoot weight were reduced significantly over untreated control at 500 J2 and above. Root weight increased at 500 and 1000 levels, though it was similar to the control at 2000 level due to poor root development at this level. The number of galls and egg masses of M. incognita and final population of predator increased with increasing inoculum level of M. incognita.

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The complete mitochondrial genome of Koerneria sudhausi (Diplogasteromorpha: Nematoda) supports monophyly of Diplogasteromorpha within Rhabditomorpha

Kim¹,

Kim²,

Nadler

et al. 2015

Curr Genet

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Testing hypotheses of monophyly for different nematode groups in the context of broad representation of nematode diversity is central to understanding the patterns and processes of nematode evolution. Herein sequence information from mitochondrial genomes is used to test the monophyly of diplogasterids, which includes an important nematode model organism. The complete mitochondrial genome sequence of Koerneria sudhausi, a representative of Diplogasteromorpha, was determined and used for phylogenetic analyses along with 60 other nematode species. The mtDNA of K. sudhausi is comprised of 16,005 bp that includes 36 genes (12 protein-coding genes, 2 ribosomal RNA genes and 22 transfer RNA genes) encoded in the same direction. Phylogenetic trees inferred from amino acid and nucleotide sequence data for the 12 protein-coding genes strongly supported the sister relationship of K. sudhausi with Pristionchus pacificus, supporting Diplogasteromorpha. The gene order of K. sudhausi is identical to that most commonly found in members of the Rhabditomorpha + Ascaridomorpha + Diplogasteromorpha clade, with an exception of some tRNA translocations. Both the gene order pattern and sequence-based phylogenetic analyses support a close relationship between the diplogasterid species and Rhabditomorpha. The nesting of the two diplogasteromorph species within Rhabditomorpha is consistent with most molecular phylogenies for the group, but inconsistent with certain morphology-based hypotheses that asserted phylogenetic affinity between diplogasteromorphs and tylenchomorphs. Phylogenetic analysis of mitochondrial genome sequences strongly supports monophyly of the diplogasteromorpha.

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Laboratory studies on the biocontrol potential of the predatory nematode Koerneria sudhausi (Nematoda: Diplogastridae)

Cited by 8 publications

References 6 publications

A New Hope: A Hermaphroditic Nematode Enables Analysis of a Recent Whole Genome Duplication Event

A New Hope: A Hermaphroditic Nematode Enables Analysis of a Recent Whole Genome Duplication Event

Effect of prey density of meloidogyne incognita on the predation efficiency of the predator, fictor composticola (nematoda: diplogasteridae)

The complete mitochondrial genome of Koerneria sudhausi (Diplogasteromorpha: Nematoda) supports monophyly of Diplogasteromorpha within Rhabditomorpha

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