Laboratory studies on Australian isolates of Metarhizium anisopliae as a biopesticide for the cattle tick Boophilus microplus

Leemon, Diana M.; Jonsson, N.N.

doi:10.1016/j.jip.2007.07.006

Cited by 93 publications

(45 citation statements)

References 36 publications

(40 reference statements)

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“…(B.) microplus (Bittencourt et al 1995(Bittencourt et al , 1999bDutra et al 2004;Leemon and Jonsson 2008) and R. sanguineus (Garcia et al 2004 has been described. Also, the infection process of B. bassiana has been described for R. sanguineus (Kirkland et al 2004a), and B. bassiana and B. amorpha on R.…”

Section: Infection Mechanismmentioning

confidence: 99%

Entomopathogenic fungi against South American tick species

Fernandes

Bittencourt

2008

Exp Appl Acarol

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Ticks are parasites of serious concern for humans, domesticated animals and wild animals. Despite scientific advances, in South America the principal control method for ticks is the use of chemical acaricides. Indiscriminate use of these products causes environmental pollution, food contamination and development of tick resistance to acaricides. In vitro studies and field trials have demonstrated that pathogenic fungal isolates not only cause mortality of many tick species, but also reduce subsequent generations due to effects on their reproductive efficacy. Accordingly, this review presents results of several studies which were conducted in South America. Furthermore, it outlines current information on fungal pathogens of ticks and discusses the need to develop and implement effective strategies for use of entomopathogenic fungi to control ticks in the near future.

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Section: Infection Mechanismmentioning

confidence: 99%

Entomopathogenic fungi against South American tick species

Fernandes

Bittencourt

2008

Exp Appl Acarol

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“…Studies on the potential of entomopathogenic fungi as tick control agents have been conducted mainly as laboratory assays (Polar et al, 2005c;Leemon and Jonsson, 2008). M. anisopliae and B. bassiana are known to cause high mortality in various life stages of R. microplus (Bittencourt et al, 1992(Bittencourt et al, , 1994a(Bittencourt et al, ,b, 1996(Bittencourt et al, , 1997aSamish and Rehacek, 1999).…”

Section: Introductionmentioning

confidence: 99%

Virulence of Mexican isolates of entomopathogenic fungi (Hypocreales: Clavicipitaceae) upon Rhipicephalus=Boophilus microplus (Acari: Ixodidae) larvae and the efficacy of conidia formulations to reduce larval tick density under field conditions

Ángel-Sahagún

Lezama-Gutiérrez

Molina-Ochoa

et al. 2010

Veterinary Parasitology

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Skoda, S. R.; Cruz-Vázquez, C.; Lorenzoni, A. G.; Galindo-Velasco, E.; Fragoso-Sánchez, H.; and Foster, John E., "Virulence of Mexican isolates of entomopathogenic fungi (Hypocreales: Clavicipitaceae) upon Rhipicephalus = Boophilus microplus (Acari: Ixodidae) larvae and the efficacy of conidia formulations to reduce larval tick density under field conditions" (2010 a b s t r a c tThe first objective was laboratory evaluation of the virulence of 53 Mexican isolates of fungi against larvae of Rhipicephalus (Boophilus) microplus. Thirty-three isolates of Metarhizium anisopliae var. anisopliae (Metschnickoff) Sorokin (Hypocreales: Clavicipitaceae) and 20 isolates of Isaria (Paecilomyces) fumosorosea (fumosoroseus) (Wize) (Eurotiales: Trichomaceae) were tested on 7-day-old larvae under laboratory conditions. Larvae were immersed in a suspension containing 10 8 conidia/mL and the CL 50 values were estimated. Then, field tests were conducted to determine the efficacy of formulations of the isolate with the highest virulence. M. anisopliae (Ma 14 isolate) was formulated with four carriers: Tween, Celite, wheat bran, and Citroline (mineral oil) and applied on pasture beds of Cynodon plectostachyus (L.), at a dose of 2 × 10 9 CFU/m 2 . In the first trial, M. anisopliae was applied on plots naturally infested with larvae; in the second trial, tick populations in the experimental plots were eliminated and then re-infested with 20,000 * Corresponding authors. This document is a U.S. government work and is not subject to copyright in the United States. 7-day-old larvae. In the laboratory, all M. anisopliae isolates infected larvae with a mortality range between 2 and 100%; also, 13 of 20 I. fumosorosea isolates caused mortality rates between 7 and 94%. In the first field trial, 14 days post-application, conidial formulations in Celite and wheat bran caused 67.8 and 94.2% population reduction, respectively. In the second trial, the Tween formulation caused the highest larval reduction, reaching up to 61% (28 days post-application). Wheat bran formulation caused 58.3% larval reduction (21 days post-application) and was one of the most effective. The carriers and emulsifiers have a large impact on the effectiveness of conidial formulations.

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“…6A). The germinated conidia of M. anisopliae on the skin of western flower thrips showed similar changes in shape (Vestergaard et al, 1999;Leemon and Jonsson, 2007); therefore, the experimental system that involves exuviae and conidia seems to reflect the natural infection process of M. anisopliae. Further, microscopic analysis revealed the degradation and fungal penetration of the epicuticle of the wireworm after germination of M. anisopliae (Zacharuk, 1970).…”

Section: Degradation Of Radiolabeled Exuviamentioning

confidence: 96%

“…6B). Leemon and Jonsson (2007) reported that conidia of M. anisopliae produced a germ tube after incubation for 11 h at 25°C on the surface of the cattle tick (Boophilus microplus). That the delay in germination on the exuvia of stink bugs might be caused by the presence of inhibitory materials is one possibility to explain the phenomenon.…”

Section: Degradation Of Radiolabeled Exuviamentioning

confidence: 99%

Laboratory studies on the infection of stink bugs with Metarhizium anisopliae strain FRM515

Ihara

Toyama

Mishiro

et al. 2008

Appl. Entomol. Zool.

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We evaluated the effects of temperature and relative humidity (RH) on the pathogenicity of Metarhizium anisopliae strain FRM 515 to Plautia crossota stali. Low temperature decreased the pathogenicity of the fungal strain: its pathogenicity at 15°C was lower than that at higher temperatures. Mortality rates differed little among stink bugs kept at 80, 60, and 40% RH after treatment with a suspension containing 16ϫ10 8 conidia/ml; however, when 1ϫ10 7 conidia/ml was used, the LT 50 value at 40% RH was 1.7 times that at 80% RH. Among three species of stink bugs, the susceptibility of Glaucias subpunctatus to M. anisopliae strain FRM515 was similar to that of P. c. stali, whereas Halyomorpha halys was relatively resistant to infection with this strain. [35 S]Cysteine-labeled exuviae were degraded by a conidial suspension of M. anisopliae strain FRM515. The radioactivity in the medium increased from 24 to 30 h after treatment to the time when conidia germinated on the surface of the exuviae.

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Laboratory studies on Australian isolates of Metarhizium anisopliae as a biopesticide for the cattle tick Boophilus microplus

Cited by 93 publications

References 36 publications

Entomopathogenic fungi against South American tick species

Entomopathogenic fungi against South American tick species

Virulence of Mexican isolates of entomopathogenic fungi (Hypocreales: Clavicipitaceae) upon Rhipicephalus=Boophilus microplus (Acari: Ixodidae) larvae and the efficacy of conidia formulations to reduce larval tick density under field conditions

Laboratory studies on the infection of stink bugs with Metarhizium anisopliae strain FRM515

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