Laboratory‐scale production of acetoin plus diacetyl by <i>Enterobacter cloacae</i> ATCC 27613

Gupta, Kavita; Yadav, N. K.; Dhawan, Samriti

doi:10.1002/bit.260201206

Cited by 12 publications

(9 citation statements)

References 6 publications

(2 reference statements)

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“…subtilis and B. licheniformis have successfully been engineered for acetoin production, while E. cloacae has been shown to be a natural overproducer of acetoin. 6,13,40,41 The enzyme encoded by operon, acetolactate synthase and acetolactate decarboxylase, convert pyruvate into acetoin through acetolactate. Overexpression of the alsSD operon from B. subtilis in B. methanolicus MGA3 resulted in proof-of-concept for acetoin production from methanol.…”

Section: Introductionmentioning

confidence: 99%

Methanol-based acetoin production by genetically engineeredBacillus methanolicus

et al. 2020

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Section: Introductionmentioning

confidence: 99%

Methanol-based acetoin production by genetically engineeredBacillus methanolicus

et al. 2020

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“…Oddly, in 1951 a patent was issued that described the use of DA in the manufacture of synthetic latex rubber (Messer and Reynolds 1951). This manufacturing process was out-competed by developments in the petrochemical sector, but during a later oil crisis in the late ‘70’s interest in large scale laboratory production of DA was revived briefly (Gupta, Yadav et al 1978). In 1969 the oral and intraperitoneal dose determined to cause 50% mortality (LD50) in both male and female rats was determined (Colley, Gaunt et al 1969).…”

Section: Introductionmentioning

confidence: 99%

Models of toxicity of diacetyl and alternative diones

Brass

Palmer

2017

Toxicology

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Diacetyl (DA; 2,3-butanedione), with the chemical formula (CH3CO)2 is a volatile organic compound with a deep yellow color and a strong buttery flavor and aroma. These properties have made DA a particularly useful and common food flavoring ingredient. However, because of this increased occupational use, workers can be exposed to high vapor concentrations in the workplace. Despite being listed by the USFDA to be ‘generally regarded as safe’ (GRAS), multiple lines of evidence suggest that exposure to high concentrations of DA vapor causes long-term impairments in lung function with lung function testing indicating evidence of either restrictive or obstructive airway narrowing in affected individuals. A growing number of preclinical studies have now addressed the short and long-term toxicity associated with DA exposure providing further insight into the toxicity of DA and related diones. This review summarizes these observations.

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“…This method extracts the fermentation products at high pH from residual xylose before injection into the GC. This routine is a modification of the method of Kolfenbach et al 4 and is more rapid than the method of separation of diacetyl and acetoin from carbohydrates by distillation reported by Gupta et al 5 Their erroneous reports of yields of 640 mg diacetyl + acetoidg sugar are 30% higher than the theoretical maximum for Enterobacfer cloacae (ATCC 27613) and points out the need for a reliable, accurate assay for these products.…”

Section: Introductionmentioning

confidence: 97%