Laboratory-scale finned-rotor impeller for dialysis

Abstract

Dialysis of blood and other fluids may be accomplished using semi-permeable membranes. Most commonly used are the commercially available hollow-fibre dialysers, which have large priming volumes, an important consideration especially when laboratory techniques are being developed. With dialysis tubing (DT), however, priming volumes are readily controlled. To ensure adequate mass transfer, mixing of both dialysate and DT content is necessary. An impeller-based dialyser which consists of two open-boxed finned rotors facilitates both dialysate and DT content mixing. The operation of this device relies on the difference in hydrodynamic forces acting on opposite ends of the rotors, causing rotation in the vertical plane. This laboratory device was assessed via mass-transfer trials in which saline and washed-packed erythrocytes were dialysed against hypo- and hypertonic dialysate, allowing estimation of the DT's overall mass-transfer coefficient. Experimental correlation between the angular speeds of the impeller's rotation in the horizontal plane omega H and finned rotors' rotation in the vertical plane omega V were also established. Results indicate that the osmolality of the DT's content follows an exponential decay, and that omega V is strongly dependent on both omega H and the submergence depth of the impeller.