Laboratory methods of diagnosis of syphilis for the beginning of the third millennium

Wicher, Konrad; Horowitz, Harold W.; Wicher, Victoria

doi:10.1016/s1286-4579(99)80521-8

Cited by 47 publications

(30 citation statements)

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“…Moreover, the sensibility is lower at initial stage of primary syphilis and late period of disease (tertiary syphilis) in comparison with the secondary syphilis. The treponemal tests such as FTA-abs, passive hemaglutination (TPHA) or enzymelinked-immunosorbent assay (ELISA) using T. pallidum antigens are used as a confirmatory test 5,11,14 .…”

Section: Introductionmentioning

confidence: 99%

Assessment of the rapid test based on an immunochromatography technique for detecting anti-Treponema pallidum antibodies

Sato

Melo

Zerbini

et al. 2003

Rev. Inst. Med. trop. S. Paulo

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SUMMARYA rapid test based on an immunochromatography assay -Determine Syphilis TP (Abbott Lab.) for detecting specific antibodies to Treponema pallidum was evaluated against serum samples from patients with clinical, epidemiological and serological diagnosis of syphilis, patients with sexually transmitted disease other than syphilis, and individuals with negative serology for syphilis. The Determine test presented the sensitivity of 93.6%, specificity of 92.5%, and positive predictive value and negative predictive value of 95.2% and 93.7%, respectively. One serum sample from patient with recent latent syphilis showed a prozone reaction. Determine TM is a rapid assay, highly specific and easy to perform. This technique obviates the need of equipment and its diagnostic features demonstrate that it may be applicable as an alternative assay for syphilis screening under some emergency conditions or for patients living in remote localities.

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Section: Introductionmentioning

confidence: 99%

Assessment of the rapid test based on an immunochromatography technique for detecting anti-Treponema pallidum antibodies

Sato

Melo

Zerbini

et al. 2003

Rev. Inst. Med. trop. S. Paulo

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“…Neurosyphilis is diagnosed based on a combination of clinical findings and reactive serology test results in the CSF (7,16). Serum VDRL and TPHA tests and controls were positive in all our three cases.…”

Section: Discussionmentioning

confidence: 77%

“…Diagnosis of early syphilis is based on showing T. pallidum in the lesion or the lymph node (7,10) via dark field microscope (DFM), direct fluorescent antibody dying method or dying the histologic tissue sample (5,7,10). The serology tests performed in early and late stages include treponemal enzyme immunoassay (EIA) designed to detect the treponema antigen, Veneral Disease Research Laboratory (VDRL) or rapid plasma reagin (RPR) tests based on the agglutination of T. pallidum particles (TPA) or hemagglutination (TPHA) and fluorescent antibody absorbtion (FTA-abs) or non-treponemal cardiolipin (5,10).…”

Section: Discussionmentioning

confidence: 99%

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2012

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Sum maryNeurosyphilis develops in about 5% of untreated patients infected with Treponema pallidum. The spirochete disseminates systemically hours to days after inoculation. Early invasion of the central nervous system can be seen in infected patients without symptoms. Here we present three patients with varying complaints, diagnosed with neurosyphilis during diagnostic work-up. The patients had diplopia, headache and blurred vision, numbness and thinning in the feet.

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“…Dado que no es posible cultivar esta bacteria, el diagnóstico de la enfermedad requiere métodos alternativos al cultivo [12][13][14] . El estándar de oro para la detección de T. pallidum es el test de "infectividad" en conejos (en inglés rabbit infectivity test -RIT), el cual requiere disponibilidad de animales y un tiempo prolongado de análisis, por lo que resulta impracticable en el laboratorio clínico 15,16 . En la actualidad, se utilizan de rutina métodos serológicos no treponémicos (VDRL, RPR), treponémicos (fl uorescent treponemal antibody absorption-FTA-Abs; ELISA; microhemagglutination test for antibodies to Treponema pallidum, MHA-Tp) y métodos directos como la microscopia de campo oscuro (MCO), la inmunofl uorescencia directa (DFA-TP), o tests de amplifi cación de ácidos nucleicos (en inglés nucleic acid amplifi cation test-NAATs) 12,[16][17][18] .…”

Section: Unidad De Infectología (Pvt)unclassified

Diagnóstico de la infección por Treponema pallidum en pacientes con sífilis temprana y neurosífilis mediante reacción de la polimerasa en cadena

S³

et al. 2011

Rev. chil. infectol.

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Diagnóstico de la infección por Treponema pallidum en pacientes con sífi lis temprana y neurosífi lis mediante reacción de la polimerasa en cadena Patricia García C., Bruno Grassi C., Félix Fich S., Aurelio Salvo L., Luis Araya C., Fernando Abarzúa C., Julia Soto M., Helena Poggi M., Marcela Lagos L., Patricia Vásquez T., Eugenia P. León C., Carlos Pérez C. y Aniela Wozniak B. Laboratory diagnosis of Treponema pallidum infection in patients with early syphilis and neurosyphilis through a PCR-based testSyphilis is a sexually transmitted disease caused by Treponema pallidum. The diagnosis is based mainly in clinical presentation and non-specifi c assays. PCR-based diagnosis has been suggested as an attractive alternative method. The aim of this study was the validation of a PCR-based test for the diagnosis of early syphilis (ES) and neurosyphilis (NS). Clinical samples of mucocutaneous lesions and cerebrospinal fl uid (CSF) specimens from patients previously diagnosed for ES and NS respectively using an enlarged gold standard, were tested by PCR. The reaction was done using primers targeting the tpN47gene. Twenty out of 21 mucocutaneous samples from patients diagnosed with ES were positive by PCR, with a clinical sensitivity of 95%. Four out of 8 CSF samples from patients previously diagnosed with NS were positive by PCR, with a clinical sensitivity of 50%. The clinical specifi city for both ES and NS was 100%. The PCR sensitivity and specifi city for mucocutaneous samples allowed us to implement this assay in our laboratory for routine diagnosis. Although the sensitivity of the PCR in CSF was low, it may be useful to support clinical diagnosis.Key words: Treponema pallidum, syphilis, polymerase chain reaction, diagnosis. Palabras clave: Treponema pallidum, sífi lis, reacción de polimerasa en cadena, diagnóstico. Pontificia Universidad Católica de ChileDepartamento de Laboratorios Clínicos:Laboratorio de Microbiología (PGC, EPLC, AWB).Unidad Docente de Apoyo:División Ginecología y Obstetricia (FAC). Laboratorio de Biología Molecular(JSM, HPM, MLL).Departamento de Medicina Interna (CPC). Escuela de Medicina (BGC).Hospital San Juan de Dios, Santiago. Servicio de Neurología (LAC). Unidad de Infectología (PVT).Hospital Sótero Del Río, Santiago. Introducción L a sífi lis es una enfermedad de transmisión sexual, de carácter crónico, que evoluciona en fases clíni-cas: sífi lis primaria, secundaria, latente y terciaria. El agente etiológico de la sífi lis, Treponema pallidum, es una espiroqueta no cultivable 1 . Durante los últimos años se han registrado brotes de esta enfermedad en varios países, incluso en naciones desarrolladas como Canadá (National Infectious Disease Examiner.com -Syphilis spikes in Alberta, Canada-health offi cials looking for solutions. URL: http://www.examiner.com). En Alberta, una provincia de Canadá, existe una gran preocupación debido a que el número de casos de sífi lis en 1999 fue dos y en 2009 ascendió a 267 casos, probablemente debido a la promiscuidad sexual de las personas, principalmente ...

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Laboratory methods of diagnosis of syphilis for the beginning of the third millennium

Cited by 47 publications

References 59 publications

Assessment of the rapid test based on an immunochromatography technique for detecting anti-Treponema pallidum antibodies

Assessment of the rapid test based on an immunochromatography technique for detecting anti-Treponema pallidum antibodies

Nörosifiliz: Farklı Prezantasyonları

Diagnóstico de la infección por Treponema pallidum en pacientes con sífilis temprana y neurosífilis mediante reacción de la polimerasa en cadena

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