Laboratory Maintenance of <i>Nocardia</i> Species

Dhakal, Dipesh; Sohng, Jae Kyung

doi:10.1002/9780471729259.mc10f01s39

Cited by 10 publications

(6 citation statements)

References 21 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nocardia spp. serve as a valuable reservoir for the isolation of bioactive compounds with clinical significance (33,34). The current study aimed to characterize a Nocardia strain isolated from soil and to analyze its antibacterial and antifungal metabolite production.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activities of the Secondary Metabolite Extracted from a Nocardia Strain

Seratnahaei¹,

Eshraghi²,

Pakzad³

et al. 2022

JABS

View full text Add to dashboard Cite

Background & Objective: The emergence of antibiotic-resistant bacteria and limited treatment options for these infections is a challenging problem in the world. There is increasing interest in isolating bioactive molecules from actinobacteria and elucidating their antimicrobial mechanisms at the moment. The current study aimed to investigate the antimicrobial activities of a Nocardia strain (N4) that was previously isolated from the soil samples in Tehran, Iran. Materials & Methods: The cultural, morphological, and physiological characteristics of the strain were determined and the identity of the strain was confirmed using 16SrRNA gene sequencing. The strain was screened for antimicrobial metabolite production. The in vitro anti-microbial activity of the extracted metabolite was assessed by agar well diffusion and minimum inhibitory concentrations methods. Finally, the structure of the extracted metabolite was confirmed by HPLC and mass spectrometry methods. Results: The isolate shared 99% sequence similarity with Nocardia soli. The strain showed broad-spectrum antimicrobial activities against some pathogenic bacteria (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 700603, Salmonella Typhimurium ATCC 14028 and Shigella sonnei RI366) and fungi (Aspergillus niger ATCC 1015 and Aspergillus fumigatus ATCC 1022) in the agar well diffusion method. The ethyl acetate extract of the strain exhibited the highest antimicrobial activity against E. coli (MIC= 4.67±3.055 μg/mL) and K. pneumoniae (MIC=3.33±1.155 μg/mL). The metabolite showed high similarity with C2 1H28N7 O14. Conclusions: The current study confirmed the presence of a bioactive metabolite in strain N4, suggesting that this may be a promising candidate for discovering bioactive components with broad antibacterial activities against a variety of pathogens.

show abstract

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activities of the Secondary Metabolite Extracted from a Nocardia Strain

Seratnahaei¹,

Eshraghi²,

Pakzad³

et al. 2022

JABS

View full text Add to dashboard Cite

show abstract

“…The culture and genetic manipulation of Nocardia sp. CS682 were performed according to previous protocols. , DD medium containing 0.2% oatmeal, 0.2% yeast extract, 0.2% soybean meal, 0.1% calcium chloride, 1% maltose, 0.1% magnesium chloride, and 0.4% glycerol was used as the production medium. The antibiotics were added when necessary at the following concentrations: 50 μg/mL for apramycin, 100 μg/mL for ampicillin, and 150 μg/mL for thiostrepton.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Tailoring Steps of Nargenicin A1 Biosynthesis Reveals a Novel Analogue with Anticancer Activities

et al. 2020

Self Cite

View full text Add to dashboard Cite

Nargenicin A1(1) is an antibacterial macrolide with effective activity against various Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus. Due to the promising properties of this compound in inhibiting cell proliferation, immunomodulation, and the cell protective effect, there has been significant interest in this molecule. Recently, the biosynthetic gene cluster (BGC) of 1 was reported from Nocardia argentinesis and Nocardia arthritidis. In addition, two crucial enzymes involved in the formation of the core decalin moiety and postmodification of the decalin moiety by an ether bridge were characterized. This study reports on the BGC of 1 from Nocardia sp. CS682. In addition, the direct capture and heterologous expression of nar BGC from Nocardia sp. CS682 in Streptomyces venezuelae led to the production of 1. Further metabolic profiling of wild type, Nocardia sp. CS682 in optimized media (DD media) resulted in the isolation of two acetylated derivatives, 18-O-acetyl-nodusmicin and 18-O-acetyl-nargenicin. The post-PKS modification pathway in biosynthesis of 1 was also deciphered by identifying intermediates and/or in vitro enzymatic reactions of NgnP1, NgnM, and NgnO3. Different novel analogues of 1, such as compound 6, compound 7, 23-demethyl 8,13-deoxy-nodusmicin (8), 23-demethyl 8,13-deoxynargenicin (9), 8,13-deoxynodusmicin (10), and 8,13-deoxynargenicin (11), were also characterized, which extended our understanding of key post-PKS modification steps during the biosynthesis of 1. In addition, the antimicrobial and anticancer activities of selected analogues were also evaluated, whereas compound 9 was shown to exhibit potent antitumor activity by induction of G2/M cell cycle arrest, apoptosis, and autophagy.

show abstract

“…Culture and genetic manipulation of Nocardia sp. CS682 and its recombinant strains were performed following standard protocols [7,49]. Brain heart infusion (BHI) medium (Difco, Franklin Lakes, NJ, USA) was used for seed culture.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, such rare-actinobacteria have drawn huge attraction in the search of novel and effective drug leads [2,6]. Nocardia species are rare-actinobacteria with promising ability to produce diverse bioactive molecules [7,8]. These molecules possess important bioactivities such as antibacterial [9,10], cytotoxic [11], and immuno-suppressing activities [12].…”

Section: Introductionmentioning

confidence: 99%

Production of a Novel Tetrahydroxynaphthalene (THN) Derivative from Nocardia sp. CS682 by Metabolic Engineering and Its Bioactivities

et al. 2019

Self Cite

View full text Add to dashboard Cite

Nargenicin A1 is major secondary metabolite produced by Nocardia sp. CS682, with an effective antibacterial activity against various Gram-positive bacteria. Most Nocardia spp. have metabolic ability to produce compounds of diverse nature, so one-strain-many-compounds (OSMAC) approach can be applied for obtaining versatile compounds from these strains. In this study, we characterized a novel 1, 3, 6, 8-tetrahydroxynaphthalene (THN) derivative by metabolic engineering approach leading to the inactivation of nargenicin A1 biosynthesis. By using genome mining, metabolite profiling, and bioinformatics, the biosynthetic gene cluster and biosynthetic mechanism were elucidated. Further, the antibacterial, anticancer, melanin formation, and UV protective properties for isolated THN compound were performed. The compound did not exhibit significant antibacterial and cytotoxic activities, but it exhibited promising UV protection effects. Thus, metabolic engineering is an effective strategy for discovering novel bioactive molecules.

show abstract

Laboratory Maintenance of Nocardia Species

Cited by 10 publications

References 21 publications

Antimicrobial Activities of the Secondary Metabolite Extracted from a Nocardia Strain

Antimicrobial Activities of the Secondary Metabolite Extracted from a Nocardia Strain

Characterization of Tailoring Steps of Nargenicin A1 Biosynthesis Reveals a Novel Analogue with Anticancer Activities

Production of a Novel Tetrahydroxynaphthalene (THN) Derivative from Nocardia sp. CS682 by Metabolic Engineering and Its Bioactivities

Contact Info

Product

Resources

About