Laboratory identification and epidemiology of streptococcal hospital isolates

Hardy, M A; Dalton, Harry P.; Mj, Allison

doi:10.1128/jcm.8.5.534-544.1978

Cited by 9 publications

(1 citation statement)

References 24 publications

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“…The morphological characteristics were observed by light microscopy (Leica DM750) and scanning electron microscopy (Hitachi S-3400N) after cultivation on ISP 2 medium at 28 °C for 3 weeks. The process of electron micrograph preparation was as used by Stokes et al [20,21]. Gram staining was performed according to the standard procedure [22].…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Antribacter soli sp. nov., a novel actinobacterium isolated from soils of weathering dolomite

Zhang,

Gao,

Yao

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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Strain KLBMP 9083T, a novel actinobacterium, was isolated from weathered soils collected from a karst area in Anshun, Guizhou Province, PR China. The taxonomic position of strain KLBMP 9083T was studied using the polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain KLBMP 9083T formed a stabilized monophyletic clade with its closest relative strain Antribacter gilvus CGMCC 1.13856T (98.4 % 16S rRNA gene sequence similarity). The peptidoglycan hydrolysates contained alanine, glutamic acid, threonine and lysine. The polar lipids were composed of diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified phosphoglycolipid, an unidentified phospholipid and an unidentified glycolipid. The predominant menaquinones were MK-9(H8) (87.1 %), MK-9(H6) (7.3 %) and MK-9(H4) (5.6 %). The major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0. The genomic DNA G+C content was 72.3 mol%. The digital DNA–DNA hybridization and average nucleotide identity values between strain KLBMP 9083T and A. gilvus CGMCC 1.13856T were 23.4 and 79.9 %, respectively. On the basis of morphological, chemotaxonomic and phylogenetic characteristics, strain KLBMP 9083T represents a novel species of the genus Antribacter , for which the name Antribacter soli sp. nov. is proposed. The type strain is KLBMP 9083T (=CGMCC 4.7737T=NBRC 115577T).

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Section: Physiology and Chemotaxonomymentioning

confidence: 99%